Antigen Extraction and B Cell Activation Enable Identification of Rare Membrane Antigen Specific Human B Cells

Zimmermann, Maria; Rose, Natalie; Lindner, John M.; Kim, Hyein; Gonçalves, Ana Rita; Callegari, Ilaria; Syedbasha, Mohammedyaseen; Kaufmann, L; Egli, Adrian; Lindberg, Raija L. P.; Kappos, Ludwig; Traggiai, Elisabetta; Sanderson, Nicholas

doi:10.3389/fimmu.2019.00829

Cited by 19 publications

(23 citation statements)

References 34 publications

(43 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…B cell activation through antigen recognition is associated with upregulation of the surface marker CD69 (19). T cell education had no effect on the number of FO or MZ B cells expressing CD69 prior to CLP ( Figure 4G).…”

Section: Immune Education Increases Clp Induced Fo B Cell Responses Imentioning

confidence: 97%

CD4 T Follicular Helper Cells Prevent Depletion of Follicular B Cells in Response to Cecal Ligation and Puncture

et al. 2020

View full text Add to dashboard Cite

Recent studies have demonstrated that induction of a diverse repertoire of memory T cells ("immune education") affects responses to murine cecal ligation and puncture (CLP), the most widely-used animal model of sepsis. Among the documented effects of immune education on CLP are changes in T cell, macrophage and neutrophil activity, more pronounced organ dysfunction and reduced survival. Little is known, however, about the effects of CLP on B cell responses, and how these responses might be altered by immune education. Importantly, effective B cell responses are modulated by IL21 produced by CD4 + /CXCR5 + /PD1 + T follicular helper (Tfh) cells. We examined the B cell population in control and immune educated mice 24 h and 60 days after CLP. Education alone increased Tfh cells. Twenty-four hours after CLP, Tfh cells were depleted. However, this reduction was less pronounced in immune educated mice than in controls and the percentage of CD4 T cells expressing a Tfh phenotype increased in the animals. CLP did not alter splenic architecture and decreased numbers of follicular, marginal, and germinal center B cells. CLP induced changes were not, however, noted following CLP in immune educated mice. At 60 days post-CLP, numbers of follicular, germinal center and marginal zone B cells were increased; this increase was more pronounced in immune educated mice. Finally, while CLP reduced the induction of antigen specific B cells in controls, this response was maintained following CLP in immune educated mice. Our data suggest that preexisting Tfh assists in rescuing the B cell response to CLP.

show abstract

Section: Immune Education Increases Clp Induced Fo B Cell Responses Imentioning

confidence: 97%

CD4 T Follicular Helper Cells Prevent Depletion of Follicular B Cells in Response to Cecal Ligation and Puncture

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Intravital microscopy has shown the relevance of Myo1f in the extravasation, migration and deformation of the nucleus of neutrophils (Salvermoser et al, 2018), and in infections with Listeria monocytogenes, the motility of Myo1f-deficient neutrophils is reduced (Kim et al, 2006). The migration of activated B cells is important for their interaction with antigens in the inflammation sites (Zimmermann et al, 2019) or to travel to germinal centers (Mesin et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Myo1e modulates the recruitment of activated B cells to inguinal lymph nodes

Girón-Pérez

Vadillo

Schnoor

et al. 2020

Journal of Cell Science

View full text Add to dashboard Cite

The inclusion of lymphocytes in high endothelial venules and their migration to the lymph nodes are critical steps in the immune response. Cell migration is regulated by the actin cytoskeleton and myosins. Myo1e is a long-tailed class I myosin and is highly expressed in B cells, which have not been studied in the context of cell migration. By using intravital microscopy in an in vivo model and performing in vitro experiments, we studied the relevance of Myo1e for the adhesion and inclusion of activated B cells in high endothelial venules. We observed reduced expression of integrins and F-actin in the membrane protrusions of B lymphocytes, which might be explained by deficiencies in vesicular trafficking. Interestingly, the lack of Myo1e reduced the phosphorylation of focal adhesion kinase (FAK; also known as PTK2), AKT (also known as AKT1) and RAC-1, disturbing the FAK-PI3K-RAC-1 signaling pathway. Taken together, our results indicate a critical role of Myo1e in the mechanism of B-cell adhesion and migration.

show abstract

“…These results suggested that the B cells from the severe group were activated and experienced an Ab-switching process. Moreover, expression of CD69 and FOS in B cells, which are upregulated upon activation ( 17 18 ), was higher in the severe group than in the mild group ( Fig. 1E ).…”

Section: Resultsmentioning

confidence: 95%

Single Cell Transcriptomic Re-analysis of Immune Cells in Bronchoalveolar Lavage Fluids Reveals the Correlation of B Cell Characteristics and Disease Severity of Patients with SARS-CoV-2 Infection

Kim

Lee

2021

Immune Netw

View full text Add to dashboard Cite

The coronavirus disease 2019 (COVID-19) pandemic (severe acute respiratory syndrome coronavirus 2) is a global infectious disease with rapid spread. Some patients have severe symptoms and clinical signs caused by an excessive inflammatory response, which increases the risk of mortality. In this study, we reanalyzed scRNA-seq data of cells from bronchoalveolar lavage fluids of patients with COVID-19 with mild and severe symptoms, focusing on Ab-producing cells. In patients with severe disease, B cells seemed to be more activated and expressed more immunoglobulin genes compared with cells from patients with mild disease, and macrophages expressed higher levels of the TNF superfamily member B-cell activating factor but not of APRIL (a proliferation-inducing ligand). In addition, macrophages from patients with severe disease had increased pro-inflammatory features and pathways associated with Fc receptor-mediated signaling, compared with patients with mild disease. CCR2-positive plasma cells accumulated in patients with severe disease, probably because of increased CCL2 expression on macrophages from patients with severe disease. Together, these results support the hypothesis that different characteristics of B cells might be associated with the severity of COVID-19 infection.

show abstract

Antigen Extraction and B Cell Activation Enable Identification of Rare Membrane Antigen Specific Human B Cells

Cited by 19 publications

References 34 publications

CD4 T Follicular Helper Cells Prevent Depletion of Follicular B Cells in Response to Cecal Ligation and Puncture

CD4 T Follicular Helper Cells Prevent Depletion of Follicular B Cells in Response to Cecal Ligation and Puncture

Myo1e modulates the recruitment of activated B cells to inguinal lymph nodes

Single Cell Transcriptomic Re-analysis of Immune Cells in Bronchoalveolar Lavage Fluids Reveals the Correlation of B Cell Characteristics and Disease Severity of Patients with SARS-CoV-2 Infection

Contact Info

Product

Resources

About