Antigen-induced release of histamine from serosal mast cells, lung, and tracheal tissue: Variation with tissue and rat strain in relation to serum IgE-antibody level

Bergstrand, Håkan; Björnsson, Anette; Frick, Inga-Maria; Lundquist, Britta; Nyström, Irene; Pauwels, Romain; Bazin, Hervé

doi:10.1007/bf01971480

Cited by 10 publications

(12 citation statements)

References 29 publications

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“…The heterogeneity of the mast cell population in the rat lung could partially ex plain the differences observed in the magnitude of an tigen-induced histamine release in the different com partments of the rat lung [31,32], The differences in mast cell populations and their distribution in the lung of the rat and guinea pig could explain the differ ences in bronchosensitivity and in spontaneous or provoked histamine release observed in both species [10,11,23].…”

Section: Discussionmentioning

confidence: 99%

Distribution and Histochemical Characterization of Pulmonary Mast Cells in the Rat and Guinea Pig

Bachelet¹,

Bernaudin²,

Fleury‐Feith³

1988

Int Arch Allergy Immunol

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The rat and guinea pig are widely used in experimental models dealing with immuno allergic bronchoreactivity. The present study was designed to compare the distribution and heterogeneity of the mast cell population in the respiratory tract of the Sprague-Dawley rat and Hartley guinea pig. Mast cells were identified according to the sequential Alcian blue/safranin·staining method. From the trachea to the peripheral conductive airways, the density of mast cells increased in the guinea pig whereas it decreased in the rat. Although mast cells were observed in the interalveolar septa of the guinea pig, none were found in the rat. In the lung of the rat, three types of mast cells were observed: in the wall of the trachea and large bronchi, safranin-positive, mixed and Alcian-blue-positive mast cells were found, whereas only Alcian-blue-positive mast cells were observed in the small conductive airways. In contrast, only Alcian-blue-positive mast cells were observed in the lung of the guinea pig. These results show that the types and localization of the mast cell populations are distinctly different in these two species. Consequently, experimental models of bronchoreactivity in these species are testing different cellular systems.

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Section: Discussionmentioning

confidence: 99%

Distribution and Histochemical Characterization of Pulmonary Mast Cells in the Rat and Guinea Pig

Bachelet¹,

Bernaudin²,

Fleury‐Feith³

1988

Int Arch Allergy Immunol

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“…Conforming to pre vious results [19,20], the correlations found were not convincing under any of the conditions examined (see table II). With cells from animals injected with silica gel as an adjuvant, there is a complete failure of corre lation between responsiveness to OA and OA-lgE or OA-IgG?« antibody levels and between responsive ness to ConA or anti-IgE and IgE levels.…”

Section: Are Serosal Mast Cell Sensitizing Homocytotropic Antibodies mentioning

confidence: 98%

“…This dose of OA is the ap proximate optimal one with respect to mast cell responsiveness [19]. The adjuvants used were 100 mg of a preparation of dried AI(OH)3 (F2200; Reheis Chemical Company, supplied by AB Astra, Sodertiilje, Sweden), 10 mg of amorphous silica gel (Aerosol 200; Degussa, Frankfurt, FRG), or two ampoules of Perthydral® (Institute Pasteur.…”

Section: Antigens and Immunizationmentioning

confidence: 99%

“…Whether this is due to a nonserosal mast cell type, e.g., a so-called mucosal mast cell, in rat lung tissue is not clarified. Neither serosal mast cell nor lung tissue re sponsiveness to antigen could be unequivocally corre lated with serum levels of IgE or IgG2« antibody [19], nor could the degree of anti-IgE or concanavalin A (ConA) induced histamine release be unequivocally correlated with total IgE serum levels [20], We also found that mast cells from PVG rats immunized with a low dose of ovalbumin together with silica gel did not respond with histamine release to antigen chal lenge; however, these mast cells turned into respond ing cells, if the animals were separately injected with alum without antigen [21]. Perthydral (BP vaccine) could not precipitate/enhance mast cell responsive ness [21], but considerably enhanced IgE (antibody) synthesis [21][22][23].…”

Section: Introductionmentioning

confidence: 99%

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Modulatory Effects of Freund’s Adjuvant Treatment on Mast Cell Histamíne Release and Homocytotropic Antibody Synthesis

Bergstrand

Andersson

Pauwels³

et al. 1985

Int Arch Allergy Immunol

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The present study examines the influence of Freund’s complete adjuvant (FCA) injections on sensitized PVG rats with respect to (1) serum levels of IgE and IgG_2α antibodies and total IgE (all assessed by radioimmunoassays) and (2) the capacity of serosal mast cells to release histamine on challenge in vitro with ‘immunological’ secretagogues (specific antigen, anti-IgE, concanavalin A) or with compound 48/80. The rats were immunized with 10 μg ovalbumin (OA); alum, Bordetella pertussis vaccine, or silica gel were employed as adjuvants. Treatment with FCA was performed by single intraperitoneal injections 3, 2, or 1 week(s) before or 1 or 2 weeks after sensitization. Tests were conducted 3 weeks after sensitization. The results show that the effect of FCA treatment varied reproducibly with the adjuvant employed for sensitization and with the timing of the FCA administration. FCA treatment could either increase, fail to affect, or decrease total serum IgE and OA-IgG_2α antibody levels as well as serosal mast cell responsiveness, whereas OA-IgE antibody responses were decreased or not affected. Moreover, serum levels of OA-IgE and OA-IgG_2α antibodies and total IgE were affected by FCA treatment independently of each other. Finally, serosal mast cell responsiveness to a given secretagogue could be influenced by the FCA treatment apparently independently of that to other secretagogues. A salient finding was that effects of FCA treatment on mast cell responsiveness did not necessarily conform to effects on antibody synthesis. Collectively, these data support the opinion that the mechanisms of action of the IgE-promoting adjuvants employed differ and suggest that the expression of serosal mast cell responsiveness to each examined secretagogue can be regulated separately. They also suggest that the serosal mast cell sensitizing capacity of homocytotropic antibodies may not be adequately quantified by immunochemical methods employing reagents prepared against IgE and IgG_2α protein.

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“…assay [1][2][3][4][5][6]. One explanation for this could be that serum levels of total IgE influence sensitization of mast cells by occupying IgE receptors.…”

Section: Introductionmentioning

confidence: 99%

Anti-IgE-induced histamine release from rat tissues passively sensitizedin vivo with myeloma IgE differs with strain and mast cell source

Bergstrand¹,

Björnsson²,

Frick³

et al. 1984

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Four different strains of rats, BDII, E3, LE, and OM/N, each with a low basal serum level of IgE, were examined with respect to anti-IgE- and ConA-induced release of histamine from serosal mast cells and chopped lung tissue. Tests were performed before and three days after i.p. injection of a graded dose of myeloma IgE (IR 162)-containing ascitic fluid. There was a clearcut strain difference in response capacity with respect to ConA- and anti-IgE-induced release of histamine from serosal mast cells before myeloma IgE-injection. Response capacity increased in some but not all strains after myeloma IgE injection; increase in response capacity of serosal mast cells did not correlate to that of chopped lung tissue. Analogous findings were observed when two of the strains, LE and E3, were passively sensitized by i.p. injection with serum containing another myeloma IgE (IR2). These results indicate that differences exist between mast cells of different rat strains, and within strain between mast cells of various tissues in capacity to become sensitized to myeloma IgE.

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Antigen-induced release of histamine from serosal mast cells, lung, and tracheal tissue: Variation with tissue and rat strain in relation to serum IgE-antibody level

Cited by 10 publications

References 29 publications

Distribution and Histochemical Characterization of Pulmonary Mast Cells in the Rat and Guinea Pig

Distribution and Histochemical Characterization of Pulmonary Mast Cells in the Rat and Guinea Pig

Modulatory Effects of Freund’s Adjuvant Treatment on Mast Cell Histamíne Release and Homocytotropic Antibody Synthesis

Anti-IgE-induced histamine release from rat tissues passively sensitizedin vivo with myeloma IgE differs with strain and mast cell source

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