Antigen-Induced Release of Histamine from Rat Tissues in vitro: Dissociation in Development of Serosal Mast Cell, Lung Tissue, and Tracheal Tissue Response Capacity

Bergstrand, Håkan; Frick, Inga-Maria; Lindhqvist, B; Lundqvist, Britta; Nyström, Irene; Schürmann, A.

doi:10.1159/000233124

Cited by 14 publications

(19 citation statements)

References 15 publications

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“…The heterogeneity of the mast cell population in the rat lung could partially ex plain the differences observed in the magnitude of an tigen-induced histamine release in the different com partments of the rat lung [31,32], The differences in mast cell populations and their distribution in the lung of the rat and guinea pig could explain the differ ences in bronchosensitivity and in spontaneous or provoked histamine release observed in both species [10,11,23].…”

Section: Discussionmentioning

confidence: 98%

Distribution and Histochemical Characterization of Pulmonary Mast Cells in the Rat and Guinea Pig

Bachelet¹,

Bernaudin²,

Fleury‐Feith³

1988

Int Arch Allergy Immunol

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The rat and guinea pig are widely used in experimental models dealing with immuno allergic bronchoreactivity. The present study was designed to compare the distribution and heterogeneity of the mast cell population in the respiratory tract of the Sprague-Dawley rat and Hartley guinea pig. Mast cells were identified according to the sequential Alcian blue/safranin·staining method. From the trachea to the peripheral conductive airways, the density of mast cells increased in the guinea pig whereas it decreased in the rat. Although mast cells were observed in the interalveolar septa of the guinea pig, none were found in the rat. In the lung of the rat, three types of mast cells were observed: in the wall of the trachea and large bronchi, safranin-positive, mixed and Alcian-blue-positive mast cells were found, whereas only Alcian-blue-positive mast cells were observed in the small conductive airways. In contrast, only Alcian-blue-positive mast cells were observed in the lung of the guinea pig. These results show that the types and localization of the mast cell populations are distinctly different in these two species. Consequently, experimental models of bronchoreactivity in these species are testing different cellular systems.

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Section: Discussionmentioning

confidence: 98%

Distribution and Histochemical Characterization of Pulmonary Mast Cells in the Rat and Guinea Pig

Bachelet¹,

Bernaudin²,

Fleury‐Feith³

1988

Int Arch Allergy Immunol

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“…In separate experiments [14], it had pre viously been checked that the degree of antigen-induced histamine release did not markedly vary with cell concentration. After chal lenge, the tubes were incubated at 37°C for 5 min.…”

Section: Antigen- Cona- Anti-ige- and Compound-48/80-induced Releamentioning

confidence: 99%

“…We previously examined the temporal development of serosal mast cell and lung tissue response capacity to antigen after sensitization of animals with various IgE-promoting adjuvants. Unexpectedly, serosal mast cell responsiveness was found to develop inde pendently of that of lung or tracheal tissue [14]. Whether this is due to a nonserosal mast cell type, e.g., a so-called mucosal mast cell, in rat lung tissue is not clarified.…”

Section: Introductionmentioning

confidence: 97%

“…[1][2][3][4][5][6][7][8][9][10][11][12]. Assays of these antibodies initially relied on passive cutaneous anaphylaxis tests, but the identifi cation of monoclonal plasmacytoma derived IgE in the rat made possible the development of assays for IgE and specific IgE antibody based on radioimmunological techniques [3,7], Reaginic (IgE) antibody re sponses are optimally induced with minute amounts of antigen when administered together with a suitable adjuvant, e.g., alum, Bordetella pertussis (BP) vaccine, or silica gel [e.g., 1,2,5,7,13,14], Most authors find Freund's complete adjuvant (FCA) less efficacious [for reviews see refs. 1-3, 5, 7], Responses induced with the aid of BP vaccine or silica gel are generally transient in nature; alum may promote more longlasting and also boosterable responses.…”

Section: Introductionmentioning

confidence: 99%

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Modulatory Effects of Freund’s Adjuvant Treatment on Mast Cell Histamíne Release and Homocytotropic Antibody Synthesis

Bergstrand

Andersson

Pauwels³

et al. 1985

Int Arch Allergy Immunol

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The present study examines the influence of Freund’s complete adjuvant (FCA) injections on sensitized PVG rats with respect to (1) serum levels of IgE and IgG_2α antibodies and total IgE (all assessed by radioimmunoassays) and (2) the capacity of serosal mast cells to release histamine on challenge in vitro with ‘immunological’ secretagogues (specific antigen, anti-IgE, concanavalin A) or with compound 48/80. The rats were immunized with 10 μg ovalbumin (OA); alum, Bordetella pertussis vaccine, or silica gel were employed as adjuvants. Treatment with FCA was performed by single intraperitoneal injections 3, 2, or 1 week(s) before or 1 or 2 weeks after sensitization. Tests were conducted 3 weeks after sensitization. The results show that the effect of FCA treatment varied reproducibly with the adjuvant employed for sensitization and with the timing of the FCA administration. FCA treatment could either increase, fail to affect, or decrease total serum IgE and OA-IgG_2α antibody levels as well as serosal mast cell responsiveness, whereas OA-IgE antibody responses were decreased or not affected. Moreover, serum levels of OA-IgE and OA-IgG_2α antibodies and total IgE were affected by FCA treatment independently of each other. Finally, serosal mast cell responsiveness to a given secretagogue could be influenced by the FCA treatment apparently independently of that to other secretagogues. A salient finding was that effects of FCA treatment on mast cell responsiveness did not necessarily conform to effects on antibody synthesis. Collectively, these data support the opinion that the mechanisms of action of the IgE-promoting adjuvants employed differ and suggest that the expression of serosal mast cell responsiveness to each examined secretagogue can be regulated separately. They also suggest that the serosal mast cell sensitizing capacity of homocytotropic antibodies may not be adequately quantified by immunochemical methods employing reagents prepared against IgE and IgG_2α protein.

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“…However, such an explanation is not supported by the finding that parasite-induced non-specific IgE does not protect against allergic reactions [12]. Moreover, response capacity of serosal mast cells to antigen does not correlate to that of lung tissue [4,6,13]. Thus, e.g.…”

Section: Introductionmentioning

confidence: 96%

Anti-IgE-induced histamine release from rat tissues passively sensitizedin vivo with myeloma IgE differs with strain and mast cell source

Bergstrand¹,

Björnsson²,

Frick³

et al. 1984

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Four different strains of rats, BDII, E3, LE, and OM/N, each with a low basal serum level of IgE, were examined with respect to anti-IgE- and ConA-induced release of histamine from serosal mast cells and chopped lung tissue. Tests were performed before and three days after i.p. injection of a graded dose of myeloma IgE (IR 162)-containing ascitic fluid. There was a clearcut strain difference in response capacity with respect to ConA- and anti-IgE-induced release of histamine from serosal mast cells before myeloma IgE-injection. Response capacity increased in some but not all strains after myeloma IgE injection; increase in response capacity of serosal mast cells did not correlate to that of chopped lung tissue. Analogous findings were observed when two of the strains, LE and E3, were passively sensitized by i.p. injection with serum containing another myeloma IgE (IR2). These results indicate that differences exist between mast cells of different rat strains, and within strain between mast cells of various tissues in capacity to become sensitized to myeloma IgE.

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Antigen-Induced Release of Histamine from Rat Tissues in vitro: Dissociation in Development of Serosal Mast Cell, Lung Tissue, and Tracheal Tissue Response Capacity

Cited by 14 publications

References 15 publications

Distribution and Histochemical Characterization of Pulmonary Mast Cells in the Rat and Guinea Pig

Distribution and Histochemical Characterization of Pulmonary Mast Cells in the Rat and Guinea Pig

Modulatory Effects of Freund’s Adjuvant Treatment on Mast Cell Histamíne Release and Homocytotropic Antibody Synthesis

Anti-IgE-induced histamine release from rat tissues passively sensitizedin vivo with myeloma IgE differs with strain and mast cell source

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