Antigen presenting cells treated in vitro by macrophage colony-stimulating factor and autoantigen protect mice from autoimmunity

Guan, Yangtai; Yu, Shuo; Zhao, Zhao; Ćirić, Bogoljub; Zhang, Guang‐Xian; Rostami, Abdolmohamad

doi:10.1016/j.jneuroim.2007.09.021

Cited by 15 publications

(11 citation statements)

References 43 publications

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“…Moser (78) also reported that Ag-pulsed macrophages could generate primed T cells in vivo but, in comparison to DC, selectively activated Th2 responses. Finally, extending the discussion above of macrophage-mediated suppression, Guan et al (79) reported that CSF-1-treated peritoneal macrophages exerted Ag-specific suppression of alloreactivity and autoimmune responses in mice.…”

Section: Macrophages Can Present Ag In a Primary Responsementioning

confidence: 64%

Macrophages as APC and the Dendritic Cell Myth

Hume

2008

The Journal of Immunology

439

359

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Dendritic cells have been considered an immune cell type that is specialized for the presentation of Ag to naive T cells. Considerable effort has been applied to separate their lineage, pathways of differentiation, and effectiveness in Ag presentation from those of macrophages. This review summarizes evidence that dendritic cells are a part of the mononuclear phagocyte system and are derived from a common precursor, responsive to the same growth factors (including CSF-1), express the same surface markers (including CD11c), and have no unique adaptation for Ag presentation that is not shared by other macrophages.

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Section: Macrophages Can Present Ag In a Primary Responsementioning

confidence: 64%

Macrophages as APC and the Dendritic Cell Myth

Hume

2008

The Journal of Immunology

439

359

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“…The APCs used in our study and in the studies described above all direct T cells to produce less IFN-␥ [38,40,41]. However, in contrast to myelin-laden macrophages, the tolerogenic APCs used in the other studies skew T cells to produce increased IL-10 levels [38,40] and decreased IL-17A levels [40]. Despite decreased IL-10 and unaffected IL-17A production by myelin-laden macrophage-activated T cells, myelinladen MOG-pulsed macrophages could still suppress EAE.…”

Section: Figure 6 Endogenous Myelin Antigens Do Not Elicit Myelin-spmentioning

confidence: 98%

Myelin ingestion alters macrophage antigen-presenting function in vitro and in vivo

Zwam

Samsom

Nieuwenhuis

et al. 2011

Journal of Leukocyte Biology

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During MS, phagocytosing myelin-containing macrophages arise and lie in close proximity to T cells. To date, it has not been addressed whether these myelin-laden macrophages have the capacity to present antigens to T cells and whether this contributes to inflammation in disease. We demonstrate that in vitro-generated human and mouse myelin-laden macrophages expressed MHC class I and II and costimulatory molecules and are thus well equipped for antigen presentation. Human myelin-laden macrophages exhibited normal endocytosis of particulate and soluble antigens. In addition, human myelin-laden macrophages elicited active T cell proliferation of naïve as well as memory T cells. Furthermore, mouse myelin-laden macrophages induced primary antigen-specific CD4(+) T cell proliferation in vivo but transiently diminished IFN-γ release. Functionally, MOG peptide-loaded myelin-laden mouse macrophages modestly but significantly reduced the severity of MOG peptide-induced EAE. These data show that myelin uptake results in the induction of a population of macrophages that retains antigen-presenting capacity and limits autoimmune-mediated disease.

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“…Cells were washed with PBS and resuspended in RPMI 1640 supplemented with 10% FBS. We have previously shown that this preparation procedure yielded > 95% pure CD11b + CD11c − macrophages with only 1.8% CD11c + DCs (Guan et al, 2007). …”

Section: Methodsmentioning

confidence: 95%

“…Our present study shows for the first time the mechanism by which MOG i.v. also exerts its modulatory effects on CD11b + CD11c − peritoneal macrophages (Guan et al, 2007) and splenic macrophages (Throsby et al, 1991; Throsby et al, 1993). MOG i.v.…”

Section: Discussionmentioning

confidence: 99%

Intravenous tolerance modulates macrophage classical activation and antigen presentation in experimental autoimmune encephalomyelitis

Li¹,

Ćirić²,

Yang³

et al. 2009

Journal of Neuroimmunology

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Macrophages act as the first line of self defense by mounting an inflammatory response to antigen and as antigen presenting cells to initiate the adaptive immune response. Inhibition of macrophage activation is one of the possible approaches to modulate inflammation. Intravenous (i.v.) tolerance has proved to be an effective method for ameliorating experimental autoimmune diseases. Whether macrophages are involved in tolerance induction is still largely undefined. In the present study we found that i.v. tolerance induction resulted in lower B7.1, B7.2 and MHC class II molecules, and reduced phagocytosis by both peritoneal macrophages and adherent splenocytes. Macrophages from tolerized mice were associated with a significantly impaired response of MOG-sensitized T cells to MOG. Macrophages from tolerized mice produced low levels of pro-inflammatory molecules IL-12, TNF-α, IL-1β, RANTES and MCP-1 and high levels of IL-10 and TGF-β. Administration of anti-TGF-β led to a reduction of IL-10 in tolerized mice. Thus, i.v. tolerance inhibits macrophage classical activation and APC function, increases macrophage alternative activation and IL-10 and TGF-β production. These cytokines, in turn, induce enhanced production of IL-10 in macrophages in MOG i.v. mice.

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Antigen presenting cells treated in vitro by macrophage colony-stimulating factor and autoantigen protect mice from autoimmunity

Cited by 15 publications

References 43 publications

Macrophages as APC and the Dendritic Cell Myth

Macrophages as APC and the Dendritic Cell Myth

Myelin ingestion alters macrophage antigen-presenting function in vitro and in vivo

Intravenous tolerance modulates macrophage classical activation and antigen presentation in experimental autoimmune encephalomyelitis

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