2011
DOI: 10.1369/jhc.2010.957191
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Antigen Retrieval Immunohistochemistry

Abstract: SummaryAs a review for the 20th anniversary of publishing the antigen retrieval (AR) technique in this journal, the authors intend briefly to summarize developments in AR-immunohistochemistry (IHC)-based research and diagnostics, with particular emphasis on current challenges and future research directions. Over the past 20 years, the efforts of many different investigators have coalesced in extending the AR approach to all areas of anatomic pathology diagnosis and research and further have led to AR-based pro… Show more

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Cited by 235 publications
(110 citation statements)
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References 187 publications
(271 reference statements)
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“…Tumor tissues were fixed, and tissue sections were embedded in paraffin and subjected to antigen retrieval in a microwave in Tris-HCl-EDTA buffer (pH 8.0) as described previously (27). Sections were incubated with anti-CD4 and anti-CD25 antibodies (Thermo Fisher Scientific) and subsequently with horseradish peroxidase (HRP)-conjugated secondary antibodies and were counterstained with hematoxylin.…”
Section: Methodsmentioning
confidence: 99%
“…Tumor tissues were fixed, and tissue sections were embedded in paraffin and subjected to antigen retrieval in a microwave in Tris-HCl-EDTA buffer (pH 8.0) as described previously (27). Sections were incubated with anti-CD4 and anti-CD25 antibodies (Thermo Fisher Scientific) and subsequently with horseradish peroxidase (HRP)-conjugated secondary antibodies and were counterstained with hematoxylin.…”
Section: Methodsmentioning
confidence: 99%
“…In such situations, heat-or proteolysis-induced epitope retrieval can be applied to improve the signal [119,120].…”
Section: Immunohistochemistry With Tissue Slicesmentioning
confidence: 99%
“…Similar challenges are encountered for HIC of FFPE samples. Over time, many different protocols have been developed to unmask antigen epitopes [24,[51][52][53][54][55][56][57][58][59][60]. These so-called AR processes are still not well understood in terms of the associated molecular changes.…”
Section: In-source Decay Approachmentioning
confidence: 99%
“…The most reasonable hypothesis is that AR allows changes in the conformation of cross-linked proteins and thus facilitates access to sites within the proteins. To date, many different AR protocols have been published [24,[51][52][53][54][55][56][57][58][59][60]. However, according to the nature of the tissue used and the protein (antigen) under consideration, some protocols work better than others which are not yet well understood.…”
Section: In-source Decay Approachmentioning
confidence: 99%