Antigenic analysis of Campylobacter species and an intracellular Campylobacter-like organism associated with porcine proliferative enteropathies

Abstract

Whole-cell and outer membrane preparations of Campylobacter mucosalis, C. hyointestinalis, C. jejuni, and C. coli isolated from porcine intestines were compared with preparations of intracellular Campylobacter-like organisms extracted directly from the lesions of pigs with proliferative enteropathy. By gradient polyacrylamide gel electrophoresis, outer membrane and total protein profiles of C. mucosalis, C. hyointestinalis, C. jejuni, and C. coli were significantly different from each other and from those of the Campylobacter-like organisms. Immunoblotting of these preparations with rabbit antisera or monoclonal antibodies prepared against the intracellular Campylobacter-like organisms showed strong reactions only with a 25,000- to 27,000-molecular-weight component of preparations of the intracellular organisms. Antisera to cultivable Campylobacter species isolates did not react with preparations of intracellular organisms. Isoelectric focusing of sonicated preparations showed protein profile differences and an immune-reactive component in the intracellular organisms with a pI of 4.5. This study suggests that the intracellular Campylobacter-like organism associated with proliferative enteropathy may be a novel bacterium with significant antigenic differences from the Campylobacter species previously associated with the disease.

Isolation of an intracellular bacterium from hamsters (Mesocricetus auratus) with proliferative ileitis and reproduction of the disease with a pure culture

“…As reported by other investigators (9,14,16,20), flagella were not observed in any of the light or electron microscopic preparations. The organism exhibited a dramatic motility within the cytoplasm of infected Intestine 407 cells examined by phase microscopy, indicating the presence of some mechanism for motility.…”

“…Porcine proliferative enteropathy is a disease of pigs with similar lesions and possibly the same etiology as hamster proliferative ileitis (15). Antigenic (14) and DNA (16) analysis of a preparation of intracellular organisms recovered from pigs with porcine proliferative enteropathy has suggested that the intracellular organism is unrelated to the presently described Campylobacter spp. and may not be a member of the genus Campylobacter.…”

Hepatobiliary Inflammation, Neoplasia, and Argyrophilic Bacteria in a Ferret Colony

“…Although this species was initially isolated from pigs with prolifer-ative enteritis, it is now known that that disease in pigs is caused by Lawsonia intracellularis. 29,36 Campylobacter species have also been identified by PCR from DNA extracted from bile and biliary epithelium in humans with hepatolithiasis. 23 In addition, mice experimentally infected with Campylobacter jejuni develop severe hepatitis.…”

Reproduction of porcine proliferative enteropathy with pure cultures of ileal symbiont intracellularis

“…However, use of these bacteria as oral inocula has never reproduced the specific disease (2,14). The conclusion that the intracellular bacteria in the lesions were likely to belong to a separate group of Campylobacter-like organisms requiring cells for growth was supported by immunologic and DNA studies on intracellular bacteria purified from the lesions (6,15). This led to our recent development of a successful cell culture system for the intracellular bacteria, using the rat enterocytes IEC-18 (10).…”