Antigenic and Biochemical Analysis of gB of Herpes Simplex Virus Type 1 and Type 2 and of Cross-reacting Glycoproteins Induced by Bovine Mammillitis Virus and Equine Herpesvirus Type 1

Abstract

SUMMARYAn antiserum was produced to the oligomeric form ofglycoprotein B (gB) induced by herpes simplex virus type 1 (HSV-1) strain 17. This antiserum gave a single common precipitin line in agar gel immunodiffusion with HSV-I, HSV-2, bovine mammillitis virus (BMV) and equine herpesvirus type 1 (EHV-1). It also neutralized HSV-1, HSV-2 and BMV but not EHV-1. Absorption of the antiserum with excess HSV-2 or BMV antigen resulted in an HSV-l-specific neutralizing antiserum. In immunoprecipitation, two proteins, gB and pgB, were precipitated from HSV-1-and HSV-2-infected cells and at least three from BMV-and EHV-l-infected cells. Glycoprotein B and pgB of three HSV-1 and three HSV-2 strains and the corresponding antigenically related glycoproteins of BMV-and EHV-l-infected cells were labelled with l:s I, digested with trypsin and the resulting peptides separated by two-dimensional thin-layer chromatography or high-pressure liquid chromatography. The resulting profiles were found to be almost identical, suggesting considerable structural conservation of the peptide backbone of the antigenically related glycoproteins of these four viruses.

Nucleotide Sequence and Characterization of the Marek's Disease Virus Homologue of Glycoprotein B of Herpes Simplex Virus

“…The predicted amino acid sequence of the MDV gene has characteristics of the gB genes reported for mammalian herpesviruses and is more closely related to those of the alphaherpesviruses HSV (Pellett et al, 1985 a), VZV and PRV (Robbins et al, 1987) than to those of the betaherpesvirus CMV (Cranage et al, 1986) or the gammaherpesvirus EBV (Pellett et al, 1985 b) at the amino acid sequence level. The existence of common antigenic epitopes in the gB gene products of mammalian herpesviruses has been demonstrated by several groups using both immunoblots and virus neutralization tests (Snowden et al, 1985 ;Balachandran et al, 1987). Although our results do not prove that avian and mammalian herpesviruses share common antigenic epitopes in gB, it would be surprising if they did not since our sequence analysis did not show that the mammalian herpesviruses were more closely related to each other than to MDV.…”

“…In this paper we report on the structure and entire sequence of the MDV homologue of the HSV gB. We have been guided in our decision to study gB by the fact that it is essential for infectivity (Sarmiento et al, 1979) and is highly conserved among herpesviruses (Snowden et al, 1985 ;Cranage et al, 1986;Pellett et al, 1985 b;Robbins et al, 1987;Keller et al, 1986;Whitbeck et al, 1988). More importantly, gB has been shown to induce both humoral and cell-mediated immune responses and to confer protective immunity to HSV (Cantin e t a/., 1987) and pseudorabies virus (PRV) (Marchioli et al, 1987).…”

Herpes Simplex Virus Glycoproteins Associated with Different Morphological Entities Projecting from the Virion Envelope

“…Glycoproteins antigenically related to HSV gB have been detected in a variety of animal herpesviruses (Norrild et al, 1978;Snowden et al, 1985) and in other biologically distinct human herpesviruses (Edson et al, 1985). There are striking homologies between HSV-1 gB and a putative product of Epstein-Barr virus with respect to amino acid sequence and predicted secondary structure (Pellet et al, 1985 a).…”

Identification of Cross-reacting Glycoproteins of Four Herpesviruses by Western Blotting

“…Calculation of RF values from SDS-PAGE of[ 14C]glucosaminelabelled samples allowed the localization of the dimeric forms of gB and pgB to the heavily reacting region (marked O in Fig. l) present with all the samples, including HSV-1 strain HFEM which we have previously shown to lack this typical glycosylated oligomeric form ofgB specified by all other strains of HSV-I studied (Snowden et al, 1985). This may be due to the presence of an unglycosylated oligomeric form of the HSV-1 (HFEM) gB polypeptide backbone undetectable in [14 C]glucosamine profiles of HSV-1 (HFE M)-infected cells.…”