Antigenic and genetic homogeneity of<i>Streptococcus uberis</i>strains from the bovine udder

Groschup, Martin H.; Hahn, Gabriele; Timoney, John F.

doi:10.1017/s0950268800048949

Cited by 5 publications

(3 citation statements)

References 32 publications

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“…DNA-fingerprinting of Strep. uberis was first described around 1990 [ 71 , 83 , 208 ]. One early study suggested substantial strain homogeneity among Strep.…”

Section: Streptococcus Uberismentioning

confidence: 99%

“…One early study suggested substantial strain homogeneity among Strep. uberis isolates [ 71 ], which may have been due to the fact that molecular typing methods were in their infancy and lacked discriminatory power. Since then, almost every study has shown a considerable level of heterogeneity among strains within and between herds, whether based on RAPD-typing [ 67 , 90 , 217 ], rep-PCR [ 207 ], PFGE [ 11 , 46 , 145 , 203 ], or MLST [ 151 , 193 ].…”

Section: Streptococcus Uberismentioning

confidence: 99%

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Molecular Epidemiology of Mastitis Pathogens of Dairy Cattle and Comparative Relevance to Humans

Zadoks

Middleton

McDougall³

et al. 2011

J Mammary Gland Biol Neoplasia

358

291

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Mastitis, inflammation of the mammary gland, can be caused by a wide range of organisms, including gram-negative and gram-positive bacteria, mycoplasmas and algae. Many microbial species that are common causes of bovine mastitis, such as Escherichia coli, Klebsiella pneumoniae, Streptococcus agalactiae and Staphylococcus aureus also occur as commensals or pathogens of humans whereas other causative species, such as Streptococcus uberis, Streptococcus dysgalactiae subsp. dysgalactiae or Staphylococcus chromogenes, are almost exclusively found in animals. A wide range of molecular typing methods have been used in the past two decades to investigate the epidemiology of bovine mastitis at the subspecies level. These include comparative typing methods that are based on electrophoretic banding patterns, library typing methods that are based on the sequence of selected genes, virulence gene arrays and whole genome sequencing projects. The strain distribution of mastitis pathogens has been investigated within individual animals and across animals, herds, countries and host species, with consideration of the mammary gland, other animal or human body sites, and environmental sources. Molecular epidemiological studies have contributed considerably to our understanding of sources, transmission routes, and prognosis for many bovine mastitis pathogens and to our understanding of mechanisms of host-adaptation and disease causation. In this review, we summarize knowledge gleaned from two decades of molecular epidemiological studies of mastitis pathogens in dairy cattle and discuss aspects of comparative relevance to human medicine.

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“…DNA-fingerprinting of Strep. uberis was first described around 1990 [ 71 , 83 , 208 ]. One early study suggested substantial strain homogeneity among Strep.…”

Section: Streptococcus Uberismentioning

confidence: 99%

Section: Streptococcus Uberismentioning

confidence: 99%

Molecular Epidemiology of Mastitis Pathogens of Dairy Cattle and Comparative Relevance to Humans

Zadoks

Middleton

McDougall³

et al. 2011

J Mammary Gland Biol Neoplasia

358

291

View full text Add to dashboard Cite

show abstract

“…As mentioned in previous work [ 6 ], Western blotting assays using a polyclonal anti-GB streptococci antibody evidenced two stained immunogenic proteins in Streptococcus uberis cell wall proteins’ pattern besides the expected immunogenic protein set of Streptococcus agalactiae . According to Groschup and colleagues [ 7 ], some Streptococcus uberis strains can also react with Lancefield group B serum.…”

Section: Introductionmentioning

confidence: 99%

Semi-Quantitative Method for Streptococci Magnetic Detection in Raw Milk

et al. 2016

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Bovine mastitis is the most costly disease for dairy farmers and the most frequent reason for the use of antibiotics in dairy cattle; thus, control measures to detect and prevent mastitis are crucial for dairy farm sustainability. The aim of this study was to develop and validate a sensitive method to magnetically detect Streptococcus agalactiae (a Group B streptococci) and Streptococcus uberis in raw milk samples. Mastitic milk samples were collected aseptically from 44 cows with subclinical mastitis, from 11 Portuguese dairy farms. Forty-six quarter milk samples were selected based on bacterial identification by conventional microbiology. All samples were submitted to PCR analysis. In parallel, these milk samples were mixed with a solution combining specific antibodies and magnetic nanoparticles, to be analyzed using a lab-on-a-chip magnetoresistive cytometer, with microfluidic sample handling. This paper describes a point of care methodology used for detection of bacteria, including analysis of false positive/negative results. This immunological recognition was able to detect bacterial presence in samples spiked above 100 cfu/mL, independently of antibody and targeted bacteria used in this work. Using PCR as a reference, this method correctly identified 73% of positive samples for streptococci species with an anti-S. agalactiae antibody, and 41% of positive samples for an anti-GB streptococci antibody.

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Streptococcus

Timoney¹

2004

Pathogenesis of Bacterial Infections in Animals

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Antigenic and genetic homogeneity ofStreptococcus uberisstrains from the bovine udder

Cited by 5 publications

References 32 publications

Molecular Epidemiology of Mastitis Pathogens of Dairy Cattle and Comparative Relevance to Humans

Molecular Epidemiology of Mastitis Pathogens of Dairy Cattle and Comparative Relevance to Humans

Semi-Quantitative Method for Streptococci Magnetic Detection in Raw Milk

Streptococcus

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