Antigenic and genomic homogeneity of successive Mycoplasma hominis isolates

Jensen, Lise Torp; Thorsen, Poul; Møller, Birger R.; Birkelund, Svend; Christiansen, Gunna

doi:10.1099/00222615-47-8-659

Cited by 23 publications

(14 citation statements)

References 22 publications

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“…Pulse-field gel electrophoresis (PFGE) [6,7], restriction fragment length polymorphism (RFLP) analysis [8], amplified fragment length polymorphism (AFLP) [9] and random amplified polymorphic DNA (RADP) [10] have been used to study the genetic diversity of this species. However, these methods are time-consuming, require a relatively large amount of biological material, may be difficult to reproduce and standardise between laboratories and generate results that are difficult to interpret.…”

Section: Introductionmentioning

confidence: 99%

Diversity of Mycoplasma hominis clinical isolates from Bordeaux, France, as assessed by multiple-locus variable-number tandem repeat analysis

et al. 2013

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BackgroundMycoplasma hominis is an opportunistic human mycoplasma species that can cause various urogenital infections and, less frequently, extragenital infections. The objective of this work was to study the genetic diversity of this species using a molecular typing method based on multiple-locus variable-number tandem repeat (VNTR) analysis (MLVA).ResultsThe genome content of M. hominis PG21 was analysed for tandem repeats (TRs), and five of the 130 TRs identified were selected for use in an MLVA assay. The method was based on GeneScan analysis of VNTR loci using multiplex PCR with fluorescent dyes and resolution by capillary electrophoresis. This approach was used on a collection of 210 urogenital and extragenital French clinical isolates collected between 1987 and 2009. Forty MLVA types were found. The discriminatory index of our MLVA scheme was 0.924. Using this new typing tool, persistent infection was suggested for six patients and new infection for one patient. Furthermore, mother-to-child transmission was confirmed in the two cases studied. Application of MLVA to a wide range of M. hominis isolates revealed high genotypic diversity and no obvious link between the MLVA type and the isolate year of collection, the patient’s age or sex, the anatomical origin of the isolates or resistance to antibiotics was found.ConclusionsOur MLVA scheme highlights the high genetic heterogeneity of the M. hominis species. It seems too discriminatory to be used for large epidemiological studies but has proven its usefulness for molecular studies at the individual level.

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Section: Introductionmentioning

confidence: 99%

Diversity of Mycoplasma hominis clinical isolates from Bordeaux, France, as assessed by multiple-locus variable-number tandem repeat analysis

et al. 2013

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“…reaction to development of an immune response (Jensen et al, 1998). It is also unknown whether phenotypic variation is a means by which avian mycoplasmas, particularly M. synoviae, can invade different tissues such as the synovial membrane and respiratory epithelium.…”

Section: Discussionmentioning

confidence: 99%

Role of phenotypic diversity in pathogenesis of avian mycoplasmosis

Noormohammadi

2007

Avian Pathology

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The interactions between avian mycoplasmas and their host cells are far more complex than might be anticipated from their apparent structural and functional simplicity. Phenotypic diversity in the form of reversible phase variation, antigenic variation or size variation is an adaptive mechanism that enables avian mycoplasmas to survive in a hostile and highly evolved host. Despite significant similarities between major membrane antigens of Mycoplasma gallisepticum and Mycoplasma synoviae, the molecular mechanisms that mediate phenotypic variation in these two pathogens are completely different. Throughout the years, these mechanisms have evolved side by side with their host immune system and provided mycoplasmas the capacity to colonize, invade and persist in an intricate host. In this article, recent advances in the understanding of the molecular mechanisms of phenotypic variation are reviewed, and implications of such variation in pathogenesis of the disease and development of vaccines and diagnostic assays are outlined.

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“…However, this is complicated by considerable antigenic heterogeneity between strains and species (Jensen et al, 1998;Zheng et al, 1992). Mice colonized pharyngeally with Mycoplasma pneumoniae are protected against genital tract infection with the same organism but not against M. genitalium (Furr and Taylor-Robinson, 1999;Taylor-Robinson and Furr, 2001).…”

Section: Genital Mycoplasmasmentioning

confidence: 98%