Antigenic anomaly in a naturally occurring nonfluidal strain ofCorynebacterium sepedonicum

Bishop, Andrew; Clarke, R. G.; Slack, S. A.

doi:10.1007/bf02854049

Cited by 11 publications

(4 citation statements)

References 20 publications

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“…The majority of Cms bacterial cultures on agar medium are of the mucoid colony type, although intermediate- and nonmucoid-type strains are also found [5,6]. Acidic bacterial exopolysaccharides (EPSs), which are produced in Cms, are also found in other subspecies of C .…”

Section: Introductionmentioning

confidence: 99%

Development of a Sensitive and Specific Polyclonal Antibody for Serological Detection of Clavibacter michiganensis subsp. sepedonicus

Przewodowski

Przewodowska

2017

PLoS ONE

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The quarantine bacterium Clavibacter michiganensis subsp. sepedonicus (Cms) causes bacterial ring rot (BRR) in potato but is difficult to detect, hampering the diagnosis of this disease. ELISA immunoassays have not been widely used to detect Cms because commercially available anti-Cms antibodies detect mainly EPS-producing bacteria and can fail to detect strains that do not produce EPS. In the current study, we developed a new type of polyclonal antibody that specifically detects Clavibacter michiganensis subsp. sepedonicus bacteria irrespective of their EPS level. We first found that the presence of bacterial EPS precluded quantitative measurement of bacteria by currently available immunoenzymatic methods, but that washing Cms cells with acidic and basic buffers to remove EPS before analysis successfully standardized ELISA results. We used a mix of three strains of Cms with diverse EPS levels to generate antigen for production of antibodies recognizing Cms cells with and without an EPS layer (IgG-EPS and IgG-N-EPS, respectively). The resulting IgG-N-EPS recognized almost all Cms strains tested in this work regardless of their mucoidal level. The availability of this new antibody renders immunological diagnostics of Cms more sensitive and reliable, as our newly developed antibodies can be used in many type of immunoassays. This work represents an important step forward in efforts to diagnose and prevent the spread of BRR, and the methods and solutions developed in this work are covered by six Polish, one European and one US patents.

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Section: Introductionmentioning

confidence: 99%

Development of a Sensitive and Specific Polyclonal Antibody for Serological Detection of Clavibacter michiganensis subsp. sepedonicus

Przewodowski

Przewodowska

2017

PLoS ONE

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“…The fluidal Cms strains were more reliably identified in DAS-ELISA than intermediate Cms strains. The complication with detection of intermediate Cms strains could be explained by it, that those strains not produce sufficient exopolysaccharide (EPS) or produce different EPS with comparison to fluidal strains and that is why are undetectable using antibodies made against EPS of fluidal Cms strains by means of ELISA (DE BOER 1983;BISHOP et al 1988;BAER & GUDMESTAD 1993 1).…”

Section: Resultsmentioning

confidence: 99%

“…93/85/EEC (SMITH et al 1997). According to colony morphology, Cms occurs mostly as fluidal (smooth, mucoid), but some Cms strains have been found as intermediate and non-fluidal (rough, non-mucoid) variants, too (SNIEZSKO & BONDE 1943;BISHOP et al 1988). A change in colony morphology takes place spontaneously during in vitro and in vivo growth (BAER & GUDMESTAD 1993).…”

Section: Introductionmentioning

confidence: 99%

“…A change in colony morphology takes place spontaneously during in vitro and in vivo growth (BAER & GUDMESTAD 1993). Non-fluidal strains not produce sufficient exopolysaccharide (EPS) or produce different EPS with comparison to fluidal strains and that is why detection of pathogen by immunochemical methods is complicated sometime (DE BOER 1983;BISHOP et al 1988;BAER & GUDMESTAD 1993). On the other hand, the loss of fluidal colony morphology and lack in vitro production of EPS do not affect the ability of Cms to infect and develop of bacterial ring rot foliar symptoms in eggplant and potato (WESTRA & SLACK 1992).…”

Section: Introductionmentioning

confidence: 99%

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Immunochemical, biochemical and pathogenic properties of fluidal and intermediate strains of Clavibacter michiganensis subsp. sepedonicus

Kokošková¹,

Jeřabková²

2002

Plant Prot. Sci.

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Clavibacter michiganensis subsp. sepedonicus (Cms), causing ring rot of potato, is a quarantine bacterium. According to colony morphology, Cms occurs mostly as fluidal (smooth), but sometime as intermediate (rough, i.e. less fluidal) variants, too. Commercial monoclonal antibodies (Agdia, USA) were used for determination of 40 Cms strains representing both forms. All Cms strains were reliably identified by IFAS, but atypical cells were sometime recorded in population of intermediate strains. The fluidal Cms strains were more reliably identified using DAS-ELISA and the Biolog GP MicroPlate System<sup>TM</sup> than intermediate strains. The intermediate Cms strains had decreased metabolic activity compared with fluidal strains and that is why they were identified only to the genera or to the species level or not identified. The differences among fluidal and intermediate Cms strains were recorded also in bioassay on eggplants. The intermediate Cms strains caused atypical or no symptoms with comparison to fluidal strains.

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Enzyme-Linked Immunosorbent Assay for the Detection and Identification of Plant Pathogenic Bacteria (In Particular for Erwinia amylovora and Clavibacter michiganensis subsp. sepedonicus)

Kokošková

Janse

2008

Plant Pathology

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Antigenic anomaly in a naturally occurring nonfluidal strain ofCorynebacterium sepedonicum

Cited by 11 publications

References 20 publications

Development of a Sensitive and Specific Polyclonal Antibody for Serological Detection of Clavibacter michiganensis subsp. sepedonicus

Development of a Sensitive and Specific Polyclonal Antibody for Serological Detection of Clavibacter michiganensis subsp. sepedonicus

Immunochemical, biochemical and pathogenic properties of fluidal and intermediate strains of Clavibacter michiganensis subsp. sepedonicus

Enzyme-Linked Immunosorbent Assay for the Detection and Identification of Plant Pathogenic Bacteria (In Particular for Erwinia amylovora and Clavibacter michiganensis subsp. sepedonicus)

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