Antigenic Cross-Reactivity between Borrelia burgdorferi, Borrelia recurrentis, Treponema pallidum, and Treponema phagedenis

Luther, Birgit; Moskophidis, Matthäus

doi:10.1016/s0934-8840(11)80104-0

Cited by 18 publications

(9 citation statements)

References 30 publications

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“…The samples were obtained from patients who showed clinical and laboratory conditions well known to be potential causes of false-positive reactions in the serologic diagnosis of syphilis, and the patients had been referred to our laboratory in Bologna. Twenty-five sera were from patients suffering from other clinically and microbiologically confirmed spirochetal infections (18,27,35) (19 Lyme borreliosis patients and 6 leptospirosis patients), 15 sera were from pregnant women (3), and 10 sera were from subjects suffering from autoimmune disorders (13,20) (antinuclear-antibody-positive sera).…”

Section: Methodsmentioning

confidence: 99%

Western Immunoblotting with FiveTreponema pallidumRecombinant Antigens for Serologic Diagnosis of Syphilis

Sambri¹,

Marangoni²,

Eyer³

et al. 2001

Clin Diagn Lab Immunol

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Five immunodominant Treponema pallidum recombinant polypeptides (rTpN47, rTmpA, rTpN37, rTpN17, and rTpN15) were blotted onto strips, and 450 sera (200 from blood donors, 200 from syphilis patients, and 50 potentially cross-reactive) were tested to evaluate the diagnostic performance of recombinant Western blotting (recWB) in comparison with in-house whole-cell lysate antigen-based immunoblotting (wclWB) and T. pallidum hemagglutination (MHA-TP) for the laboratory diagnosis of syphilis. None of the serum specimens from blood donors or from potential cross-reactors gave a positive result when evaluated by recWB, wclWB, or MHA-TP. The evaluation of the immunoglobulin G immune response by recWB in sera from patients with different stages of syphilis showed that rTmpA was the most frequently identified antigen (95%), whereas only 41% of the specimens were reactive to rTpN37. The remaining recombinant polypeptides were recognized as follows: rTpN47, 92.5%; rTpN17, 89.5%; and rTpN15, 67.5%. The agreement between recWB and MHA-TP was 95.0% (100% with sera from patients with latent and late disease), and the concordance between wclWB and MHA-TP was 92.0%. The overall concordance between recWB and wclWB was 97.5% (100% with sera from patients with secondary and late syphilis and 94.6 and 98.6% with sera from patients with primary and latent syphilis, respectively). The overall sensitivity of recWB was 98.8% and the specificity was 97.1% with MHA-TP as the reference method. These values for sensitivity and specificity were slightly superior to those calculated for wclWB (sensitivity, 97.1%, and specificity, 96.1%). With wclWB as the standard test, the sensitivity and specificity of recWB were 98.9 and 99.3%, respectively. These findings suggest that the five recombinant polypeptides used in this study could be used as substitutes for the whole-cell lysate T. pallidum antigens and that this newly developed recWB test is a good, easy-to-use confirmatory method for the detection of syphilis antibodies in serum.

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Section: Methodsmentioning

confidence: 99%

Western Immunoblotting with FiveTreponema pallidumRecombinant Antigens for Serologic Diagnosis of Syphilis

Sambri¹,

Marangoni²,

Eyer³

et al. 2001

Clin Diagn Lab Immunol

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“…False-positive results have been reported for patients with other spirochaetal infections or immunological disorders (e.g., systemic lupus erythematosis, rheumatoid factors, antinuclear and anti-DNA antibodies or cross-reacting antibodies such as those from Borrelia burgdorferi). 9,15,19,[21][22][23] Additionally, as many as 11% of HIV-infected persons have a biologic falsepositive serologic test. 4,6,[24][25][26] In our study, we have two HIV-seropositive sera, and they were evaluated as BFP reaction, including the FTA-ABS negative test results.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the fluorescent treponemal antibody absorption test for detection of antibodies (immunoglobulins G and M) to Treponema pallidum in serologic diagnosis of syphilis

et al. 2007

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We compared the fluorescent treponemal antibody-absorption (FTA-ABS) (immunoglobulin (Ig)G + IgM) assay with the (micro-) Treponema pallidum haemagglutination assay (TPHA), the T. pallidum particle agglutination assay (TPPA), the Murex syphilis ICE (ICE) enzyme-linked immunosorbent assay (ELISA), the Diesse Enzywell TP (TP) (ELISA) using 122 serum samples and the Western blot (WB) assay using 42 serum samples whose results were inharmonious with other tests. Additionally, the Captia syphilis-M (IgM) (ELISA) were performed. All sera had already been examined by the rapid plasma reagin (RPR) card test, a non-treponemal test and the TPHA, a treponemal test using routine screening tests. Agreements of the FTA-ABS with the TPHA test, the TPPA test, the ICE test and the TP test were 97.5%, 95.9%, 98.3% and 98.3%, respectively. The results suggest that the FTA-ABS test is a useful confirmatory test, but can be inadequate as a confirmatory test for serologic diagnosis of syphilis by giving equivocal and false-negative results even rarely.

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“…Serologische Kreuzreaktionen zwischen B. burgdorferi und T. pallidum sind von* mehreren Experimentatoren beschrieben worden (6,7,9,11,12,13). Dieses Auftreten von Kreuzreaktionen führt zu Interpretationsschwierigkeiten in der Differentialdiagnose von Syphilis und Lyme-Borreliose.…”

Section: Diskussionunclassified

“…Dieses Auftreten von Kreuzreaktionen führt zu Interpretationsschwierigkeiten in der Differentialdiagnose von Syphilis und Lyme-Borreliose. Da B. burgdorferi gemeinsame Antigendeterminanten mit anderen Spirochaeten aufweist (6), wird von verschiedenen Untersuchern eine Präabsorption der kreuzreagierenden Antikörper mit ReiterTreponemen (T. phagedenis) empfohlen (12,13,20,21,22 Diese Erkenntnis resultiert aus der Verwendung von ganzen Borrelien als "Antigen" in der Immunfluoreszenz-Technik. Da nach Absorption der kreuzreagierenden Seren eine deutlicheTiterabnahme registriert werden kann, liegt die Schlußfolgerung nahe, daß es gemeinsame Antigendeterminanten von B. burgdorferi, T. pallidum undT.phagedenisgeben muß und somit immunologische Kreuzreaktionen vorkommen können.…”

Section: Diskussionunclassified

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Die Bedeutung kreuzreagierender Antikörper für die Serodiagnostik der Lyme-Borreliose und der Syphilis

Alfen¹,

Wellensiek²

1994

LaboratoriumsMedizin

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Serodiagnosis of Lyme borreliosis and syphilis is complicated by antibodies crossreacting with common antigens of the family of Spirochetaceae. Sera from patients with syphilis often contain cross-reacting antibodies againstpartial antigens of Borrelia burgdorferi with molecular weights of80-, 66-, 41-, 39-and 12 kDa. N o cross-reactivity with Borrelia burgdorferi-specific antigen bands is observed. Sera of Lyme disease patients recognize treponemal antigens with 80-, 66-, 60-, 41-and 39 kDa molecular weight, however do not react with antigen bands characteristic ofTreponema pallidum. Serological cross-reactions between Borrelia burgdorferi and Treponema pallidum were tested using the indirect IFA (Inimuno-FluorescentAssay) andSDS-PA GE (Sodium Dodecyl Sulphate Poly-Acrylamide Gel Electrophoresis) with subsequent immunoblotting (Westernblot). After evaluation by various serological tests such äs TPHA (Treponema pallidum Haemagglutination Assay), the FTA-Abs Test (Fluorescence 12

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Antigenic Cross-Reactivity between Borrelia burgdorferi, Borrelia recurrentis, Treponema pallidum, and Treponema phagedenis

Cited by 18 publications

References 30 publications

Western Immunoblotting with FiveTreponema pallidumRecombinant Antigens for Serologic Diagnosis of Syphilis

Western Immunoblotting with FiveTreponema pallidumRecombinant Antigens for Serologic Diagnosis of Syphilis

Evaluation of the fluorescent treponemal antibody absorption test for detection of antibodies (immunoglobulins G and M) to Treponema pallidum in serologic diagnosis of syphilis

Die Bedeutung kreuzreagierender Antikörper für die Serodiagnostik der Lyme-Borreliose und der Syphilis

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