Antigenic Determinants of Salmonella for Production of “Clearance” Factor in Mouse Typhoid

Abstract

The "clearance" factor produced in the peritoneal cavity of mice immunized with killed vaccines prepared from Salmonella typhimurium or S. enteritidis was identified as the specific antibodies elicited by the 0 side chain of the cell wall polysaccharides in the organisms used as immunogens. After immunization of mice with vaccines prepared from virulent Salmonella strains, complement-dependent antibacterial antibodies in the serum and "clearance" factors in the peritoneal cavity were found to appear coincidentally, to last for more than one year, and to have the same specificity against the virulent bacterial strains. The relationship between the complement-dependent antibacterial antibodies and "clearance" factor, and the mechanisms of bactericidal action of these antibacterial agents in experimental typhoid were discussed.

Delayed Hypersensitivity in Murine Salmonellosis: Specificity of Footpad Reaction in Mice Infected with Rough Mutants of Salmonella typhimurium

“…Opsonic and bactericidal actions of antibodies are quite specific for the antigenic components of polysaccharides on the cell surfaces of the organisms (13,14). Cell-mediated protection against salmonella infection does not depend on the surface o antigenic components of the organisms (12,22).…”

Immunity to Infection, Allograft Immunity and Tumour Immunity: Parallels and Contrasts

“…Although it is clear that powerful resistance to intracellular bacteria is a function of macrophage activation, humoral antibody can promote the de-struction by macrophages of relatively small numbers of Salmonella injected intraperitoneally (Rowley et al 1968a, b, Ushiba et al 1959, Nakano et al 1970, or can potentiate the effect of activated or stimulated macrophages on Listeria (Njoku-Obi & Osebold 1962) or Brucella (Ralston & Elberg 1971 a, b).…”

Delayed-Type Hypersensitivity Antigens Detected in Culture Supernatants of <i>Salmonella</i><i>typhimurium</i>

“…Mice that have overcome the infection obtain acquired humoral and cell-mediated immunity [1], The DTH footpad re action is thought to be one of the manifestations of the acquired cell-mediated immunity after the infec tion [2], The opsonic and bactericidal actions of anti bodies are quite specific for the antigenic components of polysaccharides on the cell surfaces of the orga nisms [3,18]. However, the manifestation of DTH seems to be present in mice immunized with protein or protein-rich bacterial substances from S. typhimuri um [2,5,6,8,9,13,15,16].…”