Antigenic determinants of influenza virus hemagglutinin VIII. Topography of the antigenic regions of influenza virus hemagglutinin determined by competitive radioimmunoassay with monoclonal antibodies

Breschkin, Alan; Ahern, Julie A.; White, David O.

doi:10.1016/0042-6822(81)90142-2

Cited by 62 publications

(31 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To neutralize influenza virus IgG must bind to one of 4 or 5 antigenic sites on the HA [18]; those that bind to epitopes outside these sites do not neutralize the virus [42][43][44]. Such non-neutralizing antibodies may be active in vivo against virus or virus-infected cells by fixing complement enhancing phagocytosis or mediating lysis of infected cells by antibody-dependent cell cytotoxicity (ADCC); these mechanisms may well be important in protection and recovery from virus infections but are beyond the scope of this article.…”

Section: Discussionmentioning

confidence: 99%

Insights into neutralization of animal viruses gained from study of influenza virus

Outlaw

Dimmock

1991

Epidemiol. Infect.

View full text Add to dashboard Cite

SUMMARYIt has long been known that the binding of antibodies to viruses can result in a loss of infectivity, or neutralization, but little is understood of the mechanism or mechanisms of this process. This is probably because neutralization is a multifactorial phenomenon depending upon the nature of the virus itself, the particular antigenic site involved, the isotype of immunoglobulin and the ratio of virus to immunoglobulin (see below). Thus not only is it likely that neutralization of one virus will differ from another but that changing the circumstances of neutralization can change the mechanism itself. To give coherence to the topic we are concentrating this review on one virus, influenza type A which is itself well studied and reasonably well understood [1–3]. Reviews of the older literature can be found in references 4 to 7.

show abstract

Section: Discussionmentioning

confidence: 99%

Insights into neutralization of animal viruses gained from study of influenza virus

Outlaw

Dimmock

1991

Epidemiol. Infect.

View full text Add to dashboard Cite

show abstract

“…MAbs were screened by ELISA as previously described (Lunt et al, 1988b). The hybridoma secreting 9J3/G6 was isolated following fusion of SP2/0-Agl4 cells with spleen cells (Breschkin et al, 1981) from mice inoculated with a 10 K suspension of BTV-1-infected suckling mouse brain in Hanks' balanced salt solution. The hybridoma cell lines secreting MAbs were cloned once by limiting dilution and inoculated into pristane-primed BALB/c mice to produce ascites fluid.…”

Section: Production Of Mabsmentioning

confidence: 99%

Conformation of the VP2 protein of bluetongue virus (BTV) determines the involvement in virus neutralization of highly conserved epitopes within the BTV serogroup

White

Eaton

1990

Journal of General Virology

View full text Add to dashboard Cite

“…to study the arrangement of the epitopes of CS proteins within single molecules by using a competition binding assay (12)(13)(14). These were performed with five mAb to P. vivax derived from three different fusions, five mAb to P.falciparum from two fusions, and six mAb to P. knowlesi from a single fusion.…”

Section: Topological Analysis Of Antigenic Determinants On Cs Proteinmentioning

confidence: 99%

Circumsporozoite proteins of malaria parasites contain a single immunodominant region with two or more identical epitopes.

Zavala

Cochrane

Nardin

et al. 1983

The Journal of Experimental Medicine

299

151

View full text Add to dashboard Cite

We have used panels of monoclonal antibodies to circumsporozoite (CS) proteins of Plasmodium falciparium, P. vivax, and P. knowlesi to determine the number of topographically independent epitopes of these antigens. The results of competition binding assays indicated that single regions of the CS molecules were recognized by the homologous monoclonal antibodies. Competition binding assays were also used to study the specificity of antibodies contained in the sera of humans and monkeys that had developed sterile immunity after immunization with irradiated, intact sporozoites. We found that single monoclonal antibodies inhibited 70-95% of the specific binding of the polyclonal antibodies to crude extracts of sporozoites. It appears, therefore, that CS proteins are among the most immunogenic constituents of sporozoites, and that a single region of these molecules contains most of the immunogenic activity. An additional finding was that the immunodominant region of CS molecules is multivalent with regard to the expression of a single epitope. This was demonstrated by the ability of monomers of CS proteins to bind simultaneously two or more molecules of the same monoclonal antibody.

show abstract

Antigenic determinants of influenza virus hemagglutinin VIII. Topography of the antigenic regions of influenza virus hemagglutinin determined by competitive radioimmunoassay with monoclonal antibodies

Cited by 62 publications

References 14 publications

Insights into neutralization of animal viruses gained from study of influenza virus

Insights into neutralization of animal viruses gained from study of influenza virus

Conformation of the VP2 protein of bluetongue virus (BTV) determines the involvement in virus neutralization of highly conserved epitopes within the BTV serogroup

Circumsporozoite proteins of malaria parasites contain a single immunodominant region with two or more identical epitopes.

Contact Info

Product

Resources

About