Antigenic dietary protein guides maturation of the host immune system promoting resistance to <i>Leishmania major</i> infection in C57BL/6 mice

Amaral, Joana Ferreira do; Gomes-Santos, Ana Cristina; Paula-Silva, Josiely; Nicoli, Jacques R.; Vieira, Leda Quércia; Faria, Ana Maria Caetano; Menezes, José Elmo de

doi:10.1111/j.1365-2567.2009.03198.x

Cited by 13 publications

(12 citation statements)

References 49 publications

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“…Menezes et al ( 7 ) showed that exposure to food proteins after weaning plays a physiological role in inducing the maturation of the immune system both locally and systemically ( 7 ). Furthermore, it was demonstrated that mice fed a diet containing free amino acids from weaning to adulthood are more susceptible to Leishmania infection ( 9 ) and more resistant to induction of nasal and oral tolerance ( 10 , 11 ) as well as food allergy ( 11 ). In concert with these results, it has been shown recently that dietary proteins from solid food are responsible for the generation of the great majority of the small intestinal CD4+ Foxp3+ regulatory T cells involved in the induction of oral tolerance to food antigens ( 8 ).…”

Section: Discussionmentioning

confidence: 99%

“…In both cases, intact dietary proteins were replaced by equivalent amounts of free amino acids as nitrogen source and they were shown to influence the maturation of the immune system, particularly the gut-associated lymphoid tissue when consumed from weaning to adulthood ( 7 ). Moreover, the presence of intact dietary proteins after weaning is important for the generation of regulatory T cells in the small intestine ( 8 ) and for the full development of immune activities during adulthood such as a protective response to infection ( 9 ), induction of nasal ( 10 ) and oral tolerance as well as allergic reactions ( 11 ). Thus, the role of intact dietary proteins in the maturation of immunological activity at the early stages of life is clear.…”

Section: Introductionmentioning

confidence: 99%

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Consumption of Diet Containing Free Amino Acids Exacerbates Colitis in Mice

et al. 2017

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Dietary proteins can influence the maturation of the immune system, particularly the gut-associated lymphoid tissue, when consumed from weaning to adulthood. Moreover, replacement of dietary proteins by amino acids at weaning has been shown to impair the generation of regulatory T cells in the gut as well as immune activities such as protective response to infection, induction of oral and nasal tolerance as well as allergic responses. Polymeric and elemental diets are used in the clinical practice, but the specific role of intact proteins and free amino acids during the intestinal inflammation are not known. It is plausible that these two dietary nitrogen sources would yield distinct immunological outcomes since proteins are recognized by the immune system as antigens and amino acids do not bind to antigen-recognition receptors but instead to intracellular receptors such as mammalian target of rapamycin (mTOR). In this study, our aim was to evaluate the effects of consumption of an amino acid-containing diet (AA diet) versus a control protein-containing diet in adult mice at steady state and during colitis development. We showed that consumption of a AA diet by adult mature mice lead to various immunological changes including decrease in the production of serum IgG as well as increase in the levels of IL-6, IL-17A, TGF-β, and IL-10 in the small and large intestines. It also led to changes in the intestinal morphology, to increase in intestinal permeability, in the number of total and activated CD4+ T cells in the small intestine as well as in the frequency of proliferating cells in the colon. Moreover, consumption of AA diet during and prior to development of dextran sodium sulfate-induced colitis exacerbated gut inflammation. Administration of rapamycin during AA diet consumption prevented colitis exacerbation suggesting that mTOR activation was involved in the effects triggered by the AA diet. Therefore, our study suggests that different outcomes can result from the use of diets containing either intact proteins or free amino acids such as elemental, semielemental, and polymeric diets during intestinal inflammation. These results may contribute to the design of nutritional therapeutic intervention for inflammatory bowel diseases.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Consumption of Diet Containing Free Amino Acids Exacerbates Colitis in Mice

et al. 2017

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“…We observed that the impaired Th1 polarization in C57BL/6 Aa-fed mice is related to an immature state of antigen-presenting cells (APCs) in Aa-fed mice rather than an intrinsic defect in T cells. (Amaral et al, 2010). Phenotypical and functional analysis of APCs from Aa-fed mice revealed deficiencies in levels of costimulatory molecules (CD40 and CD80) as well as a poor ability to stimulate Th1 responses in vitro .…”

Section: Food Proteins In the Maturation Of Immune Systemmentioning

confidence: 99%

Food Components and the Immune System: From Tonic Agents to Allergens

Faria¹,

Gomes-Santos²,

Gonçalves³

et al. 2013

Front. Immunol.

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The intestinal mucosa is the major site of contact with antigens, and it houses the largest lymphoid tissue in the body. In physiological conditions, microbiota and dietary antigens are the natural sources of stimulation for the gut-associated lymphoid tissues (GALT) and for the immune system as a whole. Germ-free models have provided some insights on the immunological role of gut antigens. However, most of the GALT is not located in the large intestine, where gut microbiota is prominent. It is concentrated in the small intestine where protein absorption takes place. In this review, we will address the involvement of food components in the development and the function of the immune system. Studies in mice have already shown that dietary proteins are critical elements for the developmental shift of the immature neonatal immune profile into a fully developed immune system. The immunological effects of other food components (such as vitamins and lipids) will also be addressed. Most of the cells in the GALT are activated and local pro-inflammatory mediators are abundant. Regulatory elements are known to provide a delicate yet robust balance that maintains gut homeostasis. Usually antigenic contact in the gut induces two major immune responses, oral tolerance and production of secretory IgA. However, under pathological conditions mucosal homeostasis is disturbed resulting in inflammatory reactions such as food hypersensitivity. Food allergy development depends on many factors such as genetic predisposition, biochemical features of allergens, and a growing array of environmental elements. Neuroimmune interactions are also implicated in food allergy and they are examples of the high complexity of the phenomenon. Recent findings on the gut circuits triggered by food components will be reviewed to show that, far beyond their role as nutrients, they are critical players in the operation of the immune system in health and disease.

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“…Se a dieta que recebem é artificial e sem macromoléculas (antigenfree), esses defeitos se tornam mais severos, e os animais não chegam sequer a desenvolver linfonodos (Haury et al, 1997). Na UFMG, Menezes, Faria e colaboradores mostraram que animais criados em gaiolas comuns, abertas, expostos aos micróbios do ambiente, mas que recebem uma dieta sem proteínas, balanceada com aminoácidos, desenvolvem defeitos imunológicos similares a animais "isentos de germes" (Menezes et al, 2006;Amaral et al, 2010).…”

Section: O Microbioma E a Dietaunclassified

Diversidade Em Imunologia

Vaz

2016

Rev. Univ. Fed. Minas Gerais

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Na geração da diversidade imunológica, milhões de células (linfócitos, por exemplo) e moléculas (anticorpos) diferentes são geradas somaticamente, de novo em cada organismo vertebrado; os genes envolvidos na ativação de linfócitose na formação de anticorpos não são herdados inteiros. Linfócitos e anticorpos, diversos entre si, reagem uns com os outros e constituem uma rede complexa multiconectada (o sistema imune), na qual a complexidade é essencial. Reduções dessa diversidade (clonal) dos linfócitos (expansões oligoclonais) acarretam formas graves de doenças infecciosas, alérgicas e autoimunes. A imunologia atual está no processo de incorporar abordagens sistêmicas nas quais essa diversidade é importante nas associações com a microbiota nativa e com proteínas da dieta.

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Antigenic dietary protein guides maturation of the host immune system promoting resistance to Leishmania major infection in C57BL/6 mice

Cited by 13 publications

References 49 publications

Consumption of Diet Containing Free Amino Acids Exacerbates Colitis in Mice

Consumption of Diet Containing Free Amino Acids Exacerbates Colitis in Mice

Food Components and the Immune System: From Tonic Agents to Allergens

Diversidade Em Imunologia

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