Antigenic Differences Between Hemopoietic Stem Cells and Myeloid Progenitors

Engh, G.J. van den; Golub, Edward S.

doi:10.1084/jem.139.6.1621

Cited by 55 publications

(5 citation statements)

References 16 publications

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“…Thus, loss, covering and hiding of antigenic determinants seem to play a much more important role in lymphoid cell differentiation than previously thought. An example of disappearance of antigenic sites during differentiation was also reported to occur in mammals [41].…”

Section: Discussionmentioning

confidence: 99%

Ontogeny of Lymphoid Cell Surface Determinants in the Chicken

Albini

Wick²

1975

Int Arch Allergy Immunol

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Five antigenic lymphoid cell surface determinants (LCSD) were detected in hatched chickens using specific antisera. These LCSD were: thymus-specific surface determinants, bursa-specific non-immunoglobulin determinants, IgM-specific determinants, IgG-specific determinants, and IgA-specific determinants (ASD). Viable cell suspension of embryonic yolk sac, bursa, thymus and spleen were tested by means of indirect or direct immunofluorescent staining procedures for the presence and frequency of LCSD during maturation. Experiments performed with liver cells, brain cells and red blood cells of embryos confirmed the specificities of the antisera used for determinants present on cells of lymphoid tissues. The results showed LCSD to occur on yolk sac cells on the 5th to 7th embryonic day (ED). This suggests the presence of a stem cell pre-committed for the lymphoid cell line already in the yolk sac. Furthermore, findings are reported indicating the presence of distinct lymphoid stem cell populations or maturation stages in the yolk sac, which may be responsible for either populating the thymus or the bursa. The finding of ASD-bearing cells early in ontogenesis of the lymphoid system suggests the presence of two specificities in anti-chicken IgA sera, one of which may be directed against an antigenic site on a rudimentary immunoglobulin molecule, which becomes lost or hidden in later maturation. Studies on the bursa and the thymus show that covering, hiding, or loss of antigenic determinants plays an important role in lymphoid cell differentiation. Furthermore, the spleen is reached by B-determined stem cells as early as the bursa, but these stem cells seem not to proliferate in the former to any considerable extent until hatching. Finally, the sequence of the appearance of immunoglobulin classes as proposed by other authors is confirmed with reservations concerning IgA, and it is suggested that immunoglobulins are detectable earlier on cell surfaces than intracytoplasmatically.

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Section: Discussionmentioning

confidence: 99%

Ontogeny of Lymphoid Cell Surface Determinants in the Chicken

Albini

Wick²

1975

Int Arch Allergy Immunol

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“…Granulocytes and macrophages have a common origin (Metcalf, 1971a;Moore, Williams & Metcalf, 1972) and their progenitors are detectable by their ability to form macroscopic colonies of granulocytes and/or macrophages in agar culture (Bradley & Metcalf, 1966). These colony-forming cells (CFC) or colony-forming units in cultures (CFU-c), are considered to be derived from pluripotent stem cells (CFU-s) and are distinguishable from them by density (Worten, McCulloch & Till, 1969;Haskill, McNeill & Moore, 1970), sedimentation velocity (Worten et al, 1969), cell cycle status (Iscove, Till & McCulloch, 1970) and antigenicity (Van den Engh & Golub, 1974).…”

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confidence: 99%

The in Vitro Differentiation of Density Sub‐populations of Colony‐forming Cells Under the Influence of Different Types of Colony‐stimulating Factor

1977

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The in vitro proliferation and differentiation of myeloid progenitor cells (CFU‐c) in agar culture from CBA/Ca mouse bone marrow cells was studied. Density sub‐populations of marrow cells were obtained by equilibrium centrifugation in continuous albumin density gradients. The formation of colonies of granulocytes and/or macrophages was studied under the influence of three types of colony‐stimulating factor (CSF) from mouse lung conditioned medium CSFMLCM), post‐endotoxin mouse serum (CSFES) and from human urine (CSFHu). The effect of the sulphydryl reagent mercaptoethanol on colony development was also examined. The density distribution of CFU‐c was dependent on the type of CSF. Functional heterogeneity was found among CFU‐c with partial discrimination between progenitor cells forming pure granulocytic colonies and those forming pure macro‐phage colonies. Mercaptoethanol increased colony incidence but had no apparent effect on colony morphology or the density distribution of CFU‐c.

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“…Antigens such as Thy 1 (6) are known to be expressed in brain as well as on lymphohemopoietic cells, and many antibrain antisera crossreact with hemopoietic cells (7)(8)(9). Furthermore, antigenic crossreactivities have been reported between brain tissue and a pluripotential hemopoietic stem cell that give rise to hemopoietic colony formation in the spleen (CFU-s) (7).…”

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Pluripotential hemopoietic stem cells in adult mouse brain.

Bartlett¹

1982

Proc. Natl. Acad. Sci. U.S.A.

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Single cell suspensions ofadult mouse brain were shown to contain large numbers of pluripotential hemopoietic stem cells as detected by the ability to form hemopoietic colonies in the spleens of irradiated hosts. These colony forming unit, spleen (CFU-s) cells derived from brain gave rise to colonies identical in morphology and histology to those ofbone marrow-derived CFU-s. The average number of CFU-s obtained per 105 dissociated adult brain cells was 14, whereas other adult tissues such as lung, kidney, liver, heart, and thymus contained insignificant CFU-s levels when tested. As the level of CFU-s in adult blood is <1 per 106 nucleated cells, blood contamination does not contribute to the high levels found in adult brain. Individual spleen colonies isolated from irradiated CBA (H-2k) recipients injected with (BALB/c x CBA)F1 (H-2d x H-2k) brain cells were shown by immunofluorescence to contain cells bearing surface H-2d molecules, thus indicating that the colonies arose from the brain cell inoculum and were not endogenously derived. The surface phenotype of brainand bone marrow-derived CFU-s was found to differ in that brain CFU-s could be inhibited by prior incubation with a monoclonal antibrain antibody B2A2, whereas bone marrow CFU-s were not. Further differences were found between brain and bone marrow CFU-s in the congenitally anemic Wf/lW mice. These mice were shown to have very few CFU-s in the adult bone marrow, whereas the brain contained normal adult levels. The large number of hemopoietic stem cells in the brain may indicate an essential requirement for the continual generation of cells such as microglia or phagocytic cells, without the disruption of the blood-brain barrier.

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Antigenic Differences Between Hemopoietic Stem Cells and Myeloid Progenitors

Cited by 55 publications

References 16 publications

Ontogeny of Lymphoid Cell Surface Determinants in the Chicken

Ontogeny of Lymphoid Cell Surface Determinants in the Chicken

The in Vitro Differentiation of Density Sub‐populations of Colony‐forming Cells Under the Influence of Different Types of Colony‐stimulating Factor

Pluripotential hemopoietic stem cells in adult mouse brain.

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