Antigenic differences between the major glycoproteins of bovine herpesvirus type 1.1 and bovine encephalitis herpesvirus type 1.3

Collins, James K.; Ayers, V. K.; Whetstone, C A; Hurk, Sylvia van Drunen Littel‐van den

doi:10.1099/0022-1317-74-8-1509

Cited by 32 publications

(37 citation statements)

References 11 publications

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“…Monoclonal antibody analysis allows the identification of antigenic differences between BoHV-1 and BoHV-5 [22,32,45,76]. Glycoprotein C shows more divergence between BoHV-1 and BoHV-5 than gB and gD [22,45]. BoHV-5 gC, although not essential, regulates neurotropism in some areas of the olfactory pathway and is important for BoHV-5 neurovirulence in rabbits [21].…”

Section: Viral Glycoproteinsmentioning

confidence: 99%

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Ruminant alphaherpesviruses related to bovine herpesvirus 1

et al. 2006

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-Herpesviruses have mainly co-evolved with their hosts for millions of years. Consequently, different related host species may have been infected by various genetically related herpesviruses. Illustrating this concept, several ruminant alphaherpesviruses have been shown to form a cluster of viruses closely related to bovine herpesvirus 1 (BoHV-1): namely bovine herpesvirus 5, bubaline herpesvirus 1, caprine herpesvirus 1, cervid herpesviruses 1 and 2 and elk herpesvirus 1. These viruses share common antigenic properties and the serological relationships between them can be considered as a threat to BoHV-1 eradication programmes. BoHV-1 is a herpesvirus responsible for infectious bovine rhinotracheitis, which is a disease of major economic concern. In this article, the genetic properties of these ruminant alphaherpesviruses are reviewed on a comparative basis and the issue of interspecific recombination is assessed. The pathogenesis of these infections is described with emphasis on the host range and crossing of the host species barrier. Indeed, the non bovine ruminant species susceptible to these ruminant alphaherpesviruses may be potential BoHV-1 reservoirs. The differential diagnosis of these related infections is also discussed. In addition, available epidemiological data are used to assess the potential of cross-infection in ruminant populations. A better knowledge of these ruminant alphaherpesvirus infections is essential to successfully control infectious bovine rhinotracheitis.

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Section: Viral Glycoproteinsmentioning

confidence: 99%

“…Neutralising antibodies are raised after each alphaherpesvirus infection: for example, bovine field or experimental sera [82], sera from hyperimmunised rabbits [13] and monoclonal antibodies raised against gB, gC or gD [22,45] exhibit neutralising properties against BoHV-5.…”

Section: Serological Diagnosismentioning

confidence: 99%

Ruminant alphaherpesviruses related to bovine herpesvirus 1

et al. 2006

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“…The predominance of glycoprotein C-specific mAbs over other protein specificities was not a surprising finding since glycoprotein C is the most abundant BHV-1/5 envelope glycoprotein and is highly immunogenic (14,15). Other articles describing the production or the use of BHV-1 and BHV-5 mAbs also reported the predominance of mAbs specific to glycoprotein C over other proteins (11,12,25). Glycoprotein D and glycoprotein B are the other immunodominant viral proteins reported in some of these articles (11,12,25).…”

Section: Discussionmentioning

confidence: 90%

“…Other articles describing the production or the use of BHV-1 and BHV-5 mAbs also reported the predominance of mAbs specific to glycoprotein C over other proteins (11,12,25). Glycoprotein D and glycoprotein B are the other immunodominant viral proteins reported in some of these articles (11,12,25).…”

Section: Discussionmentioning

confidence: 99%

“…Western blot analysis of BHV-5-infected cell lysates, using individual mAbs as primary antibody revealed that mAbs 1F3, 2A6, 2F9, 2G10 and HB24L reacted with a protein of approximately 90 kDa in BHV-5-mately 97 kDa and is slightly larger than its BHV-5 counterpart (11,12). Consistent with these findings, nucleotide sequencing revealed that the BHV-5 glycoprotein C open reading frame is 50 amino acid shorter than the BHV-1 glycoprotein C open reading frame (10), explaining the differences in protein sizes observed.…”

Section: Discussionmentioning

confidence: 99%

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Production and characterization of monoclonal antibodies to a Brazilian bovine herpesvirus type 5

Oldoni

Weiblen

et al. 2004

Braz J Med Biol Res

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Antigens of a bovine herpesvirus type 5 (BHV-5), isolated from a cow with a neurological infection in Rio Grande do Sul State, Brazil, were used to immunize BALB/c mice to produce monoclonal antibodies (mAbs). Eleven hybridomas secreting mAbs directed at BHV-5 antigens were obtained after two fusions and screening of 356 hypoxanthine-aminopterin-thymidine-resistant clones. The mAbs reacted at dilutions up to 1:500 (hybridoma culture supernatant) and up to >1:10,000 (ascitic fluid) in an indirect fluorescent antibody assay (IFA) and in immunoperoxidase staining of BHV-5-infected cells. Four mAbs (1D12, 2E2, 2G10 and 4E4) showed virus-neutralizing activity against the parental BHV-5 isolate. Five mAbs (1F3, 2A6, 2F9, 2G10 and HB24L) reacted in Western immunoblotting with a protein of approximately 90 kDa. Three other mAbs (2E2, 3D6 and 4E4) reacted in IFA with antigens of a BHV-1 mutant glycoprotein Cnegative strain, demonstrating that they are directed at a viral antigen other than glycoprotein C. The eleven mAbs tested reacted with 20 BHV-5 field isolates and nine mAbs reacted with 10 BHV-1 isolates. Two mAbs (1F3 and 2F9) failed to react with BHV-1 field isolates, although they displayed a weak and nonreproducible reaction with the BHV-1 reference strain Los Angeles. These mAbs may be very useful in distinguishing between BHV-1 and BHV-5 infections since most of the traditional reagents and techniques are unable to do so. One mAb (2F9) was shown to bind to viral antigens by immunohistochemistry of histological sections of the brain of a BHV-5-infected calf. These results demonstrate that the mAbs produced here are suitable for use in a variety of immunological techniques and therefore may be useful for diagnostic and research purposes.

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Bovine herpesvirus meningoencephalitis association with infectious bovine rhinotracheitis (IBR) vaccine

et al. 1995

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During 1992, on a farm in the Tokachi district of Hokkaido, Japan, approximately 20 Holstein-Friesian calves showed neuroparalysis and died within 7-10 days after routine vaccination. Six male calves, aged about 1.5 months, were submitted to our laboratory for pathological examination and diagnosed as acute or subacute necrotizing meningoencephalitis due to bovine herpes virus (BHV) infection. The main necropsy findings included a few hemorrhages or clots, and malacic lesions localized in the cortical to subcortical area of the cerebrum. Histopathological brain lesions were characterized by laminar or focal necrosis of neurons, accompanying macrophages, polymorphonuclear cell infiltration, severe astrogliosis, and perivascular cuffing in all six calves. Nuclear basophilic inclusion bodies, which showed positive reaction with immunocytochemical staining of BHV antigen, were observed in the necrotic neurons, astroglia and oligodendroglia in five affected calves. BHV antigens were also seen in the cell bodies and cell processes of the necrotic neurons, which was indicative of cell-to-cell propagation of infection. There was a general tendency for more severe lesions to be located at the cortex to subcortex of the cerebrum. Milder lesions were observed in the cerebellum and brain stem. These findings suggest that the infectious route to the cerebrum in the present cases was through the olfactory bulbs and/or along the meninges beginning from the ethmoid bone, rather than through the trigeminal ganglia route as had been emphasized in studies dealing with experimental infection.

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Antigenic differences between the major glycoproteins of bovine herpesvirus type 1.1 and bovine encephalitis herpesvirus type 1.3

Cited by 32 publications

References 11 publications

Ruminant alphaherpesviruses related to bovine herpesvirus 1

Ruminant alphaherpesviruses related to bovine herpesvirus 1

Production and characterization of monoclonal antibodies to a Brazilian bovine herpesvirus type 5

Bovine herpesvirus meningoencephalitis association with infectious bovine rhinotracheitis (IBR) vaccine

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