Antigenic Differences in the Surfaces of Hyphae and Rhizoids in Allomyces

Abstract

Immunofluorescent techniques have demonstrated a difference in surface components of hyphae and rhizoids of Allomyces macrogynus. An antigenic component detected on the rhizoidal surface may be present, but masked, in the hyphal-wall matrix material. The system also allows visualization of the hyphal wall during aging, when changes from a smooth to a fissured surface are noted, and differences in adsorptive properties occur.

Hyphal Emergence and Outgrowth of Allomyces macrogynus in Aerated Cultures

“…From the chemical study we suggest that this layer contains glucan and protein and probably lipid. Fultz and Sussman (1966) by their immuno-fluorescent antibody technique for galactan in mature organisms detected galactan at the hyphal tip and through cracks in the outer wall. From this we conclude that the galactan that is synthesized at the later times forms part of the cementing substance in which the chitin fibres are embedded.…”

Oxine, Ferric Oxine and Copper Oxine as Inhibitors of Growth and Differentiation of Allomyces macrogynus

“…When estimating the protein content of the walls of A. macrogynus (Y ouatt 1977) it was found that the copper complexes precipitated the galactopeptide fraction (unpublished observation). Fultz and Sussman (1966) used an immunofluorescent technique to demonstrate that galactose residues were exposed in the walls of rhizoids and masked in· the hyphal walls. This would account for the selective staining of the rhizoids which was observed with iron and copper oxines.…”

Isolation and characterization of vesicles involved in hyphal tip growth of Achlya / by Terry William Hill.

“…Enzymatic disassembly reveals that there is only a small contribution of the outer amorphous glucan layer to the apical wall, and the apical fibrils are narrower and more loosely arranged than are lateral fibrils (Hunsley and Burnett, 1970;Hunsley, 1973). Further support for the gradation of materials is provided by reports of differential exposure of antigenic sites along a growing hypha (Fultz and Sussman, 1966;Hunsley and Kay, 1976) and differential staining with fluorescent brighteners (Gull and Trinci, 1974).…”