Antigenic heterogeneity of Bacteroides intermedius as recognized by monoclonal antibodies

Abstract

Four hybrid cell lines secreting monoclonal antibodies against antigens of Bacteroides intermedius were generated by fusing murine NSI cells with splenocytes from a rat immunized with B. intermedius strain OMZ248. An enzymelinked immunosorbent assay was used to analyze the distribution of the recognized antigens on 39 strains from various Bacteroides species and on 5 strains from other genera. Only Bacteroides species B. intermedius, B. loescheii, B. melaninogenicus, and B. corporis were found to express at least one of the recognized antigens. Strains of the two asaccharolytic black-pigmenting Bacteroides species were negative. Among the strains capable of binding to one or more of the monoclonal antibodies, five groups with different reactivity patterns could be distinguished. Two of the monoclonal antibodies were specific for B. intermedius. The B. intermedius strains were metabolically almost identical, expressed at least three of the recognized antigens, and fell into three distinct antibody reactivity groups, suggesting a tentative separation of this species into three new serogroups. Oral and nonoral isolates of B. intermedius were, however, not distinguished by the monoclonal antibodies. One monoclonal antibody was directed against an antigen strongly expressed on all saccharolytic black-pigmenting Bacteroides strains tested so far, thus confirming the previously noted antigenic relationship between the species which had emerged from the former B. melaninogenicus subsp. intermedius and B. melaninogenicus subsp. melaninogenicus groups.

Antimicrobial efficacy of alternative compounds for use in oral care toward biofilms from caries‐associated bacteria in vitro

“…All compounds included in this study are listed in (w/v) sucrose), as described earlier Gmür & Guggenheim, 1983;Guggenheim, Giertsen, Schüpbach, & Shapiro, 2001…”

Efficacy of gasiform ozone and photodynamic therapy on a multispecies oral biofilm in vitro

“…1.6 ml of substrate composed of 70% saliva + 30% of filtersterilized fluid universal medium supplemented with 67 mmol/L Sørensen's buffer, pH 7,2 (Ômodified fluid universal mediumÕ, mFUM) (18,19). The cell culture plate wells were inoculated with mixed cell suspensions (200 ll), prepared from equal volumes and densities of each species, and then the culture plates were incubated anaerobically at 37°C.…”

Microbiological understandings and mysteries of noma (cancrum oris)

“…Based on DNA homology groups (Johnson and Holdeman, 1983;Shah and Gharbia, 1992) and serology (Gmur and Guggenheim, 1983), P. intermedia has been divided into two species, P. intermedia and P. nigrescens. Regardless of being isolated from gingivitis, periodontal pockets or abscesses both species demonstrated a similar pathogenicity in animal experiments (Hafstrom and Dahlen, 1997).…”

“…They may occur simultaneously in the oral cavity (Matto et al, 1996) and there are several contradictory reports of P. intermedia and P. nigrescens being associated with the presence or absence of periodontal diseases (Moore et al, 1987(Moore et al, , 1991. Although there is a variety of molecular approaches to identify these two species (Gmur and Guggenheim, 1983;Johnson and Holdeman, 1983;Dix et al, 1990;Shah and Gharbia, 1992;Gharbia et al, 1994;Matto et al, 1996), it is extremely difficult if not impossible to find phenotypical characteristics that facilitate a rapid differentiation between P. intermedia and P. nigrescens in clinical trials (Gmur and Guggenheim, 1983;Shah and Gharbia, 1992). Thus further studies will have to be undertaken to determine if P. intermedia, P. nigrescens or both are present in the noma lesion.…”