Antigenic heterogeneity of the reticular meshwork in the white pulp of mouse spleen

Yoshida, K; Tamahashi, Nobuaki; Matsuura, Noboru; Takahashi, Tohru; Tachibana, Takehiko

doi:10.1007/bf00318177

Cited by 19 publications

(19 citation statements)

References 24 publications

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“…B lymphocytes were present at the periphery of the T lymphocyte accumulations. Bar 100 μm lymphocyte areas of the WP (Chilosi et al 1993;Satoh et al 1997;Van den Berg et al 1989;Yoshida et al 1991). The antigenic heterogeneity of the framework may induce the segregation of the lymphoid subclass (Satoh et al 1997;Yoshida et al 1991).…”

Section: Discussionmentioning

confidence: 97%

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Ontogeny of reticular framework of white pulp and marginal zone in human spleen: immunohistochemical studies of fetal spleens from the 17th to 40th week of gestation

et al. 2009

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The antigenic heterogeneity of the reticular framework of the white pulp (WP) and marginal zone (MZ) is well documented in the human adult spleen. The ontogeny of the WP and MZ of human fetal spleens was examined with special reference to the heterogeneity of the reticular framework. In the spleen of the 17th gestational week (gw), alpha-smooth muscle actin (alpha-SMA)-positive reticulum cells were scattered around the arterioles. From the 20th to 23rd gw, alpha-SMA-positive reticulum cells increased in number and began to form a reticular framework. An accumulation of T and B lymphocytes occurred within the framework, and a primitive WP was observed around the arterioles. At the 24th gw, antigenic diversity of the reticular framework was observed, and T and B lymphocytes were segregated in the framework. T lymphocytes were sorted into the alpha-SMA-positive reticular framework, and the periarteriolar lymphoid sheath (PALS) was formed around the arteriole. B lymphocytes aggregated in eccentric portions to the PALS and formed the lymph follicle (LF). The reticular framework of the LF was alpha-SMA-negative. MZ appeared in the alpha-SMA-positive reticular framework around the WP at the 26th gw. The PALS, LF, and MZ developed with gestational time. The reticular framework of the PALS, LF, and MZ is thus heterogeneous in the fetal spleen, and the development of the heterogeneity is related to the ontogeny of the PALS, LF, and MZ.

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Section: Discussionmentioning

confidence: 97%

“…A heterogeneous population of reticulum cells has been observed immunohistochemically in the WP of rat and mouse spleen (Van den Berg et al 1989;Yoshida et al 1991). The distribution of extracellular matrix protein in the reticular framework is also heterogeneous in the T-and Blymphocyte areas of the WP (Chilosi et al 1993).…”

Section: Introductionmentioning

confidence: 95%

Ontogeny of reticular framework of white pulp and marginal zone in human spleen: immunohistochemical studies of fetal spleens from the 17th to 40th week of gestation

et al. 2009

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“…As naïve T cells migrate along the stromal cell-lined corridors, they are likely to have to migrate over the associated DCs, ensuring extensive DC-T cell contact. It is interesting to note that lymphoid follicles contain stromal cells that are ultrastructurally similar to the cells in T zones (27,28). Recent studies have provided evidence that many of the follicular stromal cells produce the B cell attractant, BLC (29,30).…”

Section: Discussionmentioning

confidence: 99%

Coexpression of the chemokines ELC and SLC by T zone stromal cells and deletion of the ELC gene in theplt/pltmouse

Luther

Tang

Hyman

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

530

496

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The spontaneous mutant mouse strain, plt͞plt, lacks the secondary lymphoid organ chemokine (SLC)-ser gene and has disrupted trafficking of T cells and dendritic cells (DCs) to lymphoid tissues. We demonstrate here that the gene for the related chemokine, Epstein-Barr virus-induced molecule-1 ligand chemokine (ELC), is also deleted in this immunodeficient mouse strain. Using a combination of approaches, including bone marrow reconstitution and double in situ hybridization, we show in wild-type mice that ELC is expressed by T zone stromal cells that also make SLC. Smaller amounts of ELC are made by DCs, predominantly of the CD8 ؉ phenotype. We propose that ELC-and SLC-expressing T zone stromal cells play a central role in bringing naive T cells and DCs together for the initiation of immune responses.

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“…In contrast, reticular cells of the spleen display an enzyme and an antigenic heterogeneity demonstrated by the use of different antibodies identifying subsets of fibroblastic reticular cells in mouse and rat (Müller-Hermelink et al 1974;Van den Berg et al 1989;Yoshida et al 1991; see Table 3 in Balogh et al 2004 for overview). Several antibodies distinguish between reticular cells of T-and B-cell areas, suggesting that the antigenic diversity may depend upon the contact with lymphocytes.…”

Section: Discussionmentioning

confidence: 99%

The Actin-binding Protein Caldesmon Is in Spleen and Lymph Nodes Predominately Expressed by Smooth-muscle Cells, Reticular Cells, and Follicular Dendritic Cells

Köhler

2009

J Histochem Cytochem.

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Reticular cells and follicular dendritic cells (FDCs) build up a framework that underlies the compartmentalization of spleens and lymph nodes. Subpopulations of reticular cells express the smooth-muscle isoform of actin, indicative of a specialized contractile apparatus. We have investigated the distribution of the actin-binding protein caldesmon in spleen and lymph nodes of mice and rats. Caldesmon modulates contraction and regulates cell motility. Alternative splicing of transcripts from a single gene results in high-molecular-mass isoforms ( h-caldesmon) that are predominately expressed by smooth-muscle cells (SMCs), and low-molecular-mass isoforms ( I-caldesmon) that are thought to be widely distributed in non-muscle tissues, but the distribution of caldesmon in spleen and lymph nodes has not been reported. We have performed Western blot analysis and immunohistochemistry using four different antibodies against caldesmon, among these a newly developed polyclonal antibody directed against recombinant mouse caldesmon. Western blot analysis showed the preponderance of I-caldesmon in spleen and lymph nodes. Our results from immunohistochemistry demonstrate caldesmon in SMCs, as expected, but also in reticular cells and FDCs, and suggest that the isoform highly expressed by reticular cells is I-caldesmon. In spleen of SCID mice, caldesmon was expressed by reticular cells in the absence of lymphocytes.

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Antigenic heterogeneity of the reticular meshwork in the white pulp of mouse spleen

Cited by 19 publications

References 24 publications

Ontogeny of reticular framework of white pulp and marginal zone in human spleen: immunohistochemical studies of fetal spleens from the 17th to 40th week of gestation

Ontogeny of reticular framework of white pulp and marginal zone in human spleen: immunohistochemical studies of fetal spleens from the 17th to 40th week of gestation

Coexpression of the chemokines ELC and SLC by T zone stromal cells and deletion of the ELC gene in theplt/pltmouse

The Actin-binding Protein Caldesmon Is in Spleen and Lymph Nodes Predominately Expressed by Smooth-muscle Cells, Reticular Cells, and Follicular Dendritic Cells

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