Antigenic identification of virion structural proteins from infectious spleen and kidney necrosis virus

Xiong, Xiaoli; Dong, Chuanfu; Weng, Shaoping; Zhang, Jing; Zhang, Ye; He, Jianguo

doi:10.1016/j.fsi.2011.08.009

Cited by 12 publications

(4 citation statements)

References 32 publications

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“…For example, a commercial vaccine against RSIV (Aquavac IridoV, MSD Animal Health) provided only partial protection against an ISKNVgenotype [11], and cross-protection was not identifed between TRBIV and RSIV [79]. Tis is consistent with a report of the highest antigenicity for RSIV being related to the major capsid protein and the products of ORFs 054L, 055L, 101L, 117L, and 125L [80]. Note that 125L was not identifed as an ISKNV core gene with >95% aa similarity.…”

Section: Discussionsupporting

confidence: 83%

Genotypic Characterization of Infectious Spleen and Kidney Necrosis Virus (ISKNV) in Southeast Asian Aquaculture

Fusianto

Becker

Subramaniam

et al. 2023

Transboundary and Emerging Diseases

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Infectious spleen and kidney necrosis virus (ISKNV) is a species within the genus Megalocytivirus (family Iridoviridae), which causes high mortality disease in many freshwater and marine fish species. ISKNV was first reported in Asia and is an emerging threat to aquaculture with increasing global distribution, in part due to its presence in ornamental fish with clinical and subclinical infections. The species ISKNV includes three genotypes: red seabream iridovirus (RSIV), turbot reddish body iridovirus (TRBIV), and ISKNV. There is an increasing overlap in the recognized range of susceptible fish hosts and the geographic distribution of these distinct genotypes. To better understand the disease caused by ISKNV, a nucleic acid hybridization capture enrichment was used prior to sequencing to characterize whole genomes from archived clinical specimens of aquaculture and ornamental fish from Southeast Asia (n = 16). The method was suitable for tissue samples containing 2.50 × 104–4.58 × 109 ISKNV genome copies mg−1. Genome sequences determined using the hybridization capture method were identical to those obtained directly from tissues when there was sufficient viral DNA to sequence without enrichment (n = 2). ISKNV genomes from diverse locations, environments, and hosts had very high similarity and matched established genotype classifications (14 ISKNV genotype Clade 1 genomes with >98.81% nucleotide similarity). Conversely, two different genotypes were obtained at the same time and location (RSIV and ISKNV from grouper, Indonesia with 92.44% nucleotide similarity). Gene-by-gene analysis with representative ISKNV genomes identified 59 core genes within the species (>95% amino acid identity). The 14 Clade 1 ISKNV genomes in this study had 100% aa identity for 92–105 of 122 predicted genes. Despite high overall sequence similarity, phylogenetic analyses using single nucleotide polymorphisms differentiated isolates from different host species, country of origin, and time of collection. Whole genome studies of ISKNV and other megalocytiviruses enable genomic epidemiology and will provide information to enhance disease control in aquaculture.

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Section: Discussionsupporting

confidence: 83%

Genotypic Characterization of Infectious Spleen and Kidney Necrosis Virus (ISKNV) in Southeast Asian Aquaculture

Fusianto

Becker

Subramaniam

et al. 2023

Transboundary and Emerging Diseases

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“…Virion structural proteins play a crucial role in the course of viral infections and are often involved in the induction of immune responses and antigen recognition [ 23 ]. ADRV 2L and 58L were revealed to encode the viral envelope proteins, and their homologues in other ranaviruses were proven to be essential for virus infection and assembly [ 25 , 27 , 35 ].…”

Section: Discussionmentioning

confidence: 99%

“…Viral surface antigens are mainly localized to the outer envelope or capsid surface of virions [ 23 , 24 ]. Recently, Rana grylio virus (RGV) 2L and 53R were revealed to encode the viral envelope proteins, and the knockdown of 2L and 53R genes by artificial miRNAs or recombinant virus technology in RGV demonstrated that they play important roles in virus infection and assembly [ 25 , 26 , 27 ].…”

Section: Introductionmentioning

confidence: 99%

Protective Immunity Induced by DNA Vaccination against Ranavirus Infection in Chinese Giant Salamander Andrias davidianus

Chen

Gao

et al. 2018

Viruses

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Andrias davidianus ranavirus (ADRV) is an emerging viral pathogen that causes severe systemic hemorrhagic disease in Chinese giant salamanders. There is an urgent need for developing an effective vaccine against this fatal disease. In this study, DNA vaccines containing the ADRV 2L gene (pcDNA-2L) and the 58L gene (pcDNA-58L) were respectively constructed, and their immune protective effects were evaluated in Chinese giant salamanders. In vitro and in vivo expression of the vaccine plasmids were confirmed in transfected cells and muscle tissues of vaccinated Chinese giant salamanders by using immunoblot analysis or RT-PCR. Following ADRV challenge, the Chinese giant salamanders vaccinated with pcDNA-2L showed a relative percent survival (RPS) of 66.7%, which was significant higher than that in Chinese giant salamanders immunized with pcDNA-58L (RPS of 3.3%). Moreover, the specific antibody against ADRV was detected in Chinese giant salamanders vaccinated with pcDNA-2L at 14 and 21 days post-vaccination by indirect enzyme-linked immunosorbent assay (ELISA). Transcriptional analysis revealed that the expression levels of immune-related genes including type I interferon (IFN), myxovirus resistance (Mx), major histocompatibility complex class IA (MHC IA), and immunoglobulin M (IgM) were strongly up-regulated after vaccination with pcDNA-2L. Furthermore, vaccination with pcDNA-2L significantly suppressed the virus replication, which was seen by a low viral load in the spleen of Chinese giant salamander survivals after ADRV challenge. These results indicated that pcDNA-2L could induce a significant innate immune response and an adaptive immune response involving both humoral and cell-mediated immunity that conferred effective protection against ADRV infection, and might be a potential vaccine candidate for controlling ADRV disease in Chinese giant salamanders.

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“…Even though ISKNV could be easily propagated in MFF-1 cells, almost all of the previous studies have been performed by i.p. injection (Xiong et al, 2011;Xu et al, 2008Xu et al, , 2010. Go & Whittington (2006) reported virulence of dwarf gourami iridovirus (DGIV), which belongs to the ISKNV genotype, to Murray cod Maccullochella peelii peelii in a cohabitation experiment; however, they did not mention the prevalence of the virus among the organs after the virus infection.…”

Section: Discussionmentioning

confidence: 99%

Prevalence of red sea bream iridovirus among organs of Japanese amberjack (Seriola quinqueradiata) exposed to cultured red sea bream iridovirus

Ito

Yoshiura

Kamaishi

et al. 2013

Journal of General Virology

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Antigenic identification of virion structural proteins from infectious spleen and kidney necrosis virus

Cited by 12 publications

References 32 publications

Genotypic Characterization of Infectious Spleen and Kidney Necrosis Virus (ISKNV) in Southeast Asian Aquaculture

Genotypic Characterization of Infectious Spleen and Kidney Necrosis Virus (ISKNV) in Southeast Asian Aquaculture

Protective Immunity Induced by DNA Vaccination against Ranavirus Infection in Chinese Giant Salamander Andrias davidianus

Prevalence of red sea bream iridovirus among organs of Japanese amberjack (Seriola quinqueradiata) exposed to cultured red sea bream iridovirus

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