Antigenic Makeup of Subcellular Fractions of Trypanosoma cruzi

Abstract

SYNOPSIS. Trypanosoma cruzi epimastigotes from cultures were separated into nuclear, mitochondrial, lysosomal, microsomal, and cell-sap fractions. Enzymic and ultrastructural controls served to determine the cleanness of separation. The bulk of the DNA was in the nuclear (78%) and mitochondrial (12%) fractions. RN'A was found in microsomal (74%) and cell-sap ( 14% ) fractions. Marker enzyme distribution (succinic dehydrogenase, acid phosphatase, and glucose-6-phosphatase) was compared with their distribution in mammalian tissues. Subcellular localization of antigens by Ouchterlony tests revealed 2 specific precipitin lines in cell sap and 1 in the microsomal fraction. These antigens may prove diagnostically and immunoprotectively valuable.

Association between nutritional indicators and infectivity of dogs seroreactive for Trypanosoma cruzi in a rural area of northwestern Argentina

“…A total of 0.1 ml physiological solution containing 250 lg of a whole T. cruzi homogenate (Segura et al 1974) was inoculated intradermally in the internal area of one of the thighs. In addition, 0.1 ml physiological solution was inoculated in the other thigh as a control.…”

Control of Trypanosoma cruzi Epimastigote Motility through the Nitric Oxide Pathway

“…Cell cultures. Trypanosoma cruzi epimastigote forms of the Tulahuen 2 strain were cultured up to the late logaritmic phase (six days at 28°C) in a biphasic axenic medium [15].…”

“…Amino acid analogs were purchased from Research Biochemicals Incorporated (Natick, MA, USA) and components of the T. cruzi growth medium were obtained from Difco Laboratories (Detroit, MI, USA).Cell cultures. Trypanosoma cruzi epimastigote forms of the Tulahuen 2 strain were cultured up to the late logaritmic phase (six days at 28°C) in a biphasic axenic medium [15].Cell motility measurements. Cells were collected at 1,000 g for 10 min and washed three times with 0.25 M sucrose in 5 mM KCl.…”

Cell fractionation of parasitic protozoa: a review

“…Since the first protection studies with flagellar fractions (see above), it was clear that an internal component of flagella (even from monogenetic trypanosomatid species) could confer a high degree of protection against lethal challenges with T. cruzi trypomastigotes (Segura et al 1974, Pereira et al 1977, Ruiz et al 1985. Considering that the components of the axoneme are highly conserved in eukaryotes, the paraflagellar structure had the potential to be used as an immunogen for vaccination, with the advantage that it is different from any cytoskeletal structure of the mammalian host cell.…”

“…Considering that the components of the axoneme are highly conserved in eukaryotes, the paraflagellar structure had the potential to be used as an immunogen for vaccination, with the advantage that it is different from any cytoskeletal structure of the mammalian host cell. Pursuing this lead, a research group developed immunization protocols using purified major paraxial proteins with excellent results (Segura et al 1974, Wrightsman et al 1995, Miller et al 1996, 1997.…”