Antigenic Markers on Cultured Human Cells I

Franks, D.

doi:10.1159/000465173

Cited by 4 publications

(3 citation statements)

References 6 publications

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“…Haemagglutinins have been shown to exist in some malignant tissues [4], but extracts of the tumour in the present case showed no haemagglutinating activity. Alternatively, tum our-associated antigens may have stimulated the production of antibodies cross-reacting with the I antigen of autologous erythrocytes [1],…”

Section: Discussionmentioning

confidence: 99%

Cryopathic Haemolytic Anaemia Associated with Uterus Myomatosus

Kornberg¹,

Naparstek²,

Hershko³

1980

Acta Haematol

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Section: Discussionmentioning

confidence: 99%

Cryopathic Haemolytic Anaemia Associated with Uterus Myomatosus

Kornberg¹,

Naparstek²,

Hershko³

1980

Acta Haematol

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“…They are recognized by human autoantibodies, most of which are coldreactive monoclonal IgM proteins (2). However, these antigens are not confined to human erythrocytes; they have been detected on human lymphocytes, polymorphonuclear leukocytes, macrophages (3), and various cultured cells (4,5) and in water-soluble form in certain secretions (2). Moreover, they are variably expressed on erythrocytes (6) and cultured cell lines of several animal species (7,8).…”

mentioning

confidence: 99%

I and i antigens of human peripheral blood lymphocytes cocap with receptors for concanavalin A.

Feizi

Kapadia

Yount³

1980

Proc. Natl. Acad. Sci. U.S.A.

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Surface immunofluorescence experiments using a human anti-i and two anti-I antisera have been performed on human peripheral blood lymphocytes. These are known to contain cold-reactive monoclonal IgM antibodies against the carbohydrate sequence:Galfl -4GlcNAc#1 -3Galjfl 4GlcNAc/31 -3Gal-(anti-i Den) and the 1 --4, 1 -6 domain (anti-I Ma) and the 1 --4, 1 3 domain (anti-I Step) of the branched I-active structure:Galf1l 4GlcNAcfll \6G lfli -4GIcNAcfl -3Gal-A high proportion of B-and T-type lymphocytes express these I and i determinants. In the presence of anti-human immunoglobulin, the cold-reactive membrane-associated complexes of I-anti-I and i-anti-i become stabilized, and redistribution (with patching and capping) can be elicited at 370C. Dual fluorescence experiments have shown striking concordant staining of I or i (fluorescein) caps and patches with concanavalin A (rhodamine) reactive sites on normal and leukemic cells, suggesting that a proportion of I and i active structures of lymphocyte membranes are structurally associated or physiologically coupled with glycoproteins carrying oligosaccharides with branched mannosyl cores.

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“…The I antigens are well represented on the red cells of the vast majority of adults (Wiener et al, 1956); the i antigens are expressed strongly on cord blood cells and in the course of the first year of life a gradual change occurs: the I antigen increases to adult levels and the i antigen diminishes to low levels (Marsh, 1961). The I and i antigens are also present on leucocytes (Franks, 1966;Shuinak et al, 1971; Thomas, In the last several years it has become apparent that tlie I and i antigens are a family of determinants and that they occur on certain precursors of the ABH and Lewis blood group substaiiccs (Feizi et a!, 1971a, b;Feizi& Kabat, 1972). So far there is structural information on one of the I antigenic determinants.…”

mentioning

confidence: 99%

The Blood Group I and i Antigens of Amniotic Fluid

Feizi¹,

Cederqvist²,

Childs³

1975

Br J Haematol

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Human amniotic fluid has been shown to contain blood group i as well as I antigens. Crude extracts of amniotic fluids at 16-23 weeks of gestation were in general more active than those obtained at term. A pool of amniotic fluids which had A, B, H as well as I and i activity was fractionated with an insolubilized anti-I (Group 3 type) immunoadsorbent column. There was evidence for the occurrence of I and i determinants on macromolecules carrying A, B and H determinants, for the fraction specifically retained by the column was enriched by 50-60-fold in I and i activity and by at least 10-fold in A, B and H activity. In the fraction not retained by the column there remained the bulk of the A, B, H activity in addition to I activity of Group I type which is known to be distinct from the Group 3 determinant. With the aid of specific immunochemical fractionation procedures, human amniotic fluid should prove useful in structural studies of the several I and i determinants and of their relationship to other blood group determinants.

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Antigenic Markers on Cultured Human Cells I

Cited by 4 publications

References 6 publications

Cryopathic Haemolytic Anaemia Associated with Uterus Myomatosus

Cryopathic Haemolytic Anaemia Associated with Uterus Myomatosus

I and i antigens of human peripheral blood lymphocytes cocap with receptors for concanavalin A.

The Blood Group I and i Antigens of Amniotic Fluid

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