Antigenic Phenotype of Cultured Human Osteoblast-Like Cells

Reyes-Botella, Candela; Montes, María José Sánchez; Vallecillo-Capilla, Manuel; Olivares, Enrique G.; Ruíz, Concepción

doi:10.1159/000067906

Cited by 49 publications

(44 citation statements)

References 27 publications

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“…Although the immunosuppressive function of DOC is promising, it cannot be ignored that DOC gradually expressed MHC class II and lost their suppressive activity in vivo. It is reported that POC expressed all immune surface markers which can elicit an immune response, and they are able to act as APCs (42)(43)(44)(45)(46). Hence, it is predicted that DOC in vivo are going the way their progeny normally does.…”

Section: Discussionmentioning

confidence: 99%

The Immunogenicity and Immunomodulatory Function of Osteogenic Cells Differentiated from Mesenchymal Stem Cells

Liu

Kemeny

Heng

et al. 2006

The Journal of Immunology

191

165

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Multipotent mesenchymal stem cells (MSC) are reported to be immunoprivileged as well as immunosuppressive. Hence, they are ideal candidates for allogeneic transplantation to induce regeneration of diseased tissues and organs. However, it is not known whether MSC would retain their immunoprivileged and immunomodulatory properties after differentiating into the local cell types of the transplantation site. This study sought to investigate this question with a novel New Zealand White rabbit osteogenesis model. Results showed that osteogenic cells differentiated from MSC (DOC) in vitro did not express the MHC class II molecule, were incapable of inducing allogeneic lymphocyte proliferation in mixed lymphocyte culture or generating CTL, were inhibitory in ongoing lymphocyte proliferation, and secreted anti-inflammatory cytokines (IL-10 and TGF-β). There was a significantly higher secretion of IL-10 by DOC than that by MSC, while there was no significant difference between the TGF-β secretion of MSC and DOC in vitro. However, after IFN-γ treatment, TGF-β secretion by DOC significantly decreased despite the increased production by MSC. Four weeks after local DOC implantation, despite MHC class II expression, second-set allogeneic skin rejection showed similar survival to first-set allogeneic skin rejection and DOC appeared to function as osteoblasts. In conclusion, DOC retained their immunoprivileged and immunomodulatory properties in vitro, but the latter was lost following transplantation.

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Section: Discussionmentioning

confidence: 99%

The Immunogenicity and Immunomodulatory Function of Osteogenic Cells Differentiated from Mesenchymal Stem Cells

Liu

Kemeny

Heng

et al. 2006

The Journal of Immunology

191

165

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“…Older data show that osteoblasts can present antigen 98 and, based on phenotype, may even function as dendritic cells. 99 Other reports of sheep and humans using the cotransplantation of umbilical or bone marrow-derived CD34 ϩ cells, along with cultured BMSC cells, have also resulted in significantly higher engraftment levels after transplantation than was observed after transplantation of the hematopoietic cells alone. 100 These results do not appear to be dependent on the successful engraftment of stromal cells into the bone marrow, nor does the homing of CD34 ϩ cells appear to be affected by cotransplantation.…”

Section: What Is the Role Of Osteoblasts In Transplantation?mentioning

confidence: 98%

Blood and bone: two tissues whose fates are intertwined to create the hematopoietic stem-cell niche

Taichman

2005

Blood

546

370

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The mechanisms of bone and blood formation have traditionally been viewed as distinct, unrelated processes, but compelling evidence suggests that they are intertwined. Based on observations that hematopoietic precursors reside close to endosteal surfaces, it was hypothesized that osteoblasts play a central role in hematopoiesis, and it has been shown that osteoblasts produce many factors essential for the survival, renewal, and maturation of hematopoietic stem cells (HSCs). Preceding these observations are studies demonstrating that the disruption or perturbation of normal osteoblastic function has a profound and central role in defining the operational structure of the HSC niche. These observations provide a glimpse of the dimensions and ramifications of HSC-osteoblast interactions. Although more research is required to secure a broader grasp of the molecular mechanisms that govern blood and bone biology, the central role for osteoblasts in hematopoietic stem cell regulation is reviewed herein from the perspectives of (1) historical context; (2) the role of the osteoblast in supporting stem cell survival, proliferation, and maintenance; (3) the participation, if any, of osteoblasts in the creation of a stem cell niche; (4) the molecules that mediate HSC-osteoblast interactions; (5) the role of osteoblasts in stem cell transplantation; and (6) Introduction and historical perspectiveHematopoiesis occurs in unique microenvironments that facilitate the maintenance of hematopoietic stem cells (HSCs) as pluripotent and support the maturation of progenitors. Each of these activities may require different growth factors and microenvironments, the identities of which have yet to be determined. In vitro, bone marrow stromal cells (BMSCs) serve as a rich source of growth factors for a variety of hematopoietic processes. BMSCs are composed of several different populations, including fibroblasts, macrophages, endothelial cells, and adipocytes. Although it is difficult to discern the relative importance of each of these cells, it has been shown that direct stromal cell-blood cell contact, BMSC production of the extracellular bone marrow matrix, and cytokine synthesis are all relevant to the formation and maturation of blood cells in vitro. [1][2][3] The role of BMSCs in vivo is less clear.Osteoblasts have long been known to play a central role in skeletal development. Derived from pluripotent mesenchymal stem cells (MSCs), they mature along a specific lineage to become highly specialized synthetic cells. As such, osteoblasts respond to many mechanical, local, and systemic stimuli that facilitate mineralization while they orchestrate bone remodeling. Osteoblasts also constitute part of the stromal cell support system in marrow, but little is known about their functional relevance to HSCs. Early attempts to understand this relationship focused on the protective function that bone might serve for the hematopoietic organ. 4 Observations in birds of recurrent trabeculation of the medullary cavity during ovulation show that the h...

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“…28 A strong and constitutive expression of CD10 and CD44 was described on cultured human osteoblasts in flow cytometry studies [29][30][31] and on human bone tissue sections using immunohistochemistry. 32 Other authors described expression of CD44 antigen, a multifunctional adhesion molecule that binds to extracellular matrix molecules such as hyaluronate, 33 type I collagen, and fibronectin 34 during the change from osteoblast to osteocyte.…”

Section: Discussionmentioning

confidence: 99%

“…44,45 Controversy still surrounds the exact role of b2-microglobulin in normal and abnormal bone physiology due to the absence of an identified receptor and the disagreement concerning its mitogenic effect. 46 According to the results presented here, MSCs were recently described to express CD44 and HLA-I but not CD80, CD86, or HLA-II, 47 whereas adult osteoblasts were shown to express CD54, CD80, CD86, and HLA-DR. 29 Second, we investigated the interaction between fetal osteoblasts and lymphocytes. We report that fetal bone cells inhibited in vitro the proliferation of stimulated PBMCs, although with patterns not always comparable with those of adult mesenchymal and bone cells.…”

Section: Discussionmentioning

confidence: 99%

In VitroCharacterization of Immune-Related Properties of Human Fetal Bone Cells for Potential Tissue Engineering Applications

Montjovent

Bocelli‐Tyndall

Scaletta

et al. 2009

Tissue Engineering Part A

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We describe herein some immunological properties of human fetal bone cells recently tested for bone tissueengineering applications. Adult mesenchymal stem cells (MSCs) and osteoblasts were included in the study for comparison. Surface markers involved in bone metabolism and immune recognition were analyzed using flow cytometry before and after differentiation or treatment with cytokines. Immunomodulatory properties were studied on activated peripheral blood mononuclear cells (PBMCs). The immuno-profile of fetal bone cells was further investigated at the gene expression level. Fetal bone cells and adult MSCs were positive for Stro-1, alkaline phosphatase, CD10, CD44, CD54, and b2-microglobulin, but human leukocyte antigen (HLA)-I and CD80 were less present than on adult osteoblasts. All cells were negative for HLA-II. Treatment with recombinant human interferon gamma increased the presence of HLA-I in adult cells much more than in fetal cells. In the presence of activated PBMCs, fetal cells had antiproliferative effects, although with patterns not always comparable with those of adult MSCs and osteoblasts. Because of the immunological profile, and with their more-differentiated phenotype than of stem cells, fetal bone cells present an interesting potential for allogeneic cell source in tissue-engineering applications.

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Antigenic Phenotype of Cultured Human Osteoblast-Like Cells

Cited by 49 publications

References 27 publications

The Immunogenicity and Immunomodulatory Function of Osteogenic Cells Differentiated from Mesenchymal Stem Cells

The Immunogenicity and Immunomodulatory Function of Osteogenic Cells Differentiated from Mesenchymal Stem Cells

Blood and bone: two tissues whose fates are intertwined to create the hematopoietic stem-cell niche

In VitroCharacterization of Immune-Related Properties of Human Fetal Bone Cells for Potential Tissue Engineering Applications

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