Antigenic phenotypes of cultured malignant astrocytomas: Identification of lineage-consistent, lineage-independent and putative tumor-restricted antigenic expression

Jennings, Mark T.; Jennings, Vicki; Asadourian, Laura L.H.; Ebrahim, Salah A.D.; Klein, Corinna; Old, Lloyd J.

doi:10.1016/0022-510x(89)90008-7

Cited by 7 publications

(2 citation statements)

References 38 publications

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“…In the present study we used the human glial cell line SK-MG-1 which histologically had been characterized as a glioblastoma line, expressing typical glial markers such as glial fibrillary acidic protein and the gangliosides GD2 and GD3 (Jennings et al 1989). In addition, these cells had been shown to take up adrenaline by a saturable process resembling uptak% (Skalski et al 1990).…”

Section: Introductionmentioning

confidence: 99%

Expression of the extraneuronal monoamine transporter (uptake2) in human glioma cells

Streich

Brüss

Bönisch

1996

Naunyn-Schmiedeberg's Arch Pharmacol

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Tritiated methylphenylpyridinium ([3H]MPP+), a substrate of the neuronal and extraneuronal noradrenaline transporter (uptake1 and uptake2, respectively) and of the organic cation transporter (OCT1), was used to characterize the amine transport system of the established human glioma cell line SK-MG-1. Uptake of [3H]MPP+ (25 nM) into SK-MG-1 cells increased linearly with time for up to 15 min. Selective uptake1 inhibitors (e.g. (+)oxaprotiline) or omission of Na+ or Cl-ions did not affect [3H]MPP+ uptake, whereas uptake2 inhibitors such as O-methyl-isoprenaline (OMI) or corticosterone as well as depolarizing concentrations of K+ or Ba2+ strongly reduced [3H]MPP+ uptake. Initial rates of OMI(100 microM)-sensitive [3H]MPP+ uptake were saturable, with a K(m) of about 17 microM and a maximal rate of about 50 pmol/(min x mg protein). IC50 (or Ki) values for inhibition of [3H]MPP+ uptake by substrates and inhibitors of uptake2 or OCT1 were highly significantly correlated with published IC50 values for inhibition of uptake2 but not with corresponding values for inhibition of OCT1. The results presented here clearly demonstrate that human glioma cells express an uptake2 transporter. Thus, glial cells in the human central nervous system endowed with this transporter are likely to contribute to the inactivation of neuronally released noradrenaline.

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Section: Introductionmentioning

confidence: 99%

Expression of the extraneuronal monoamine transporter (uptake2) in human glioma cells

Streich

Brüss

Bönisch

1996

Naunyn-Schmiedeberg's Arch Pharmacol

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“…When syngeneic cells are used for immunization, the host is tolerant to 'self' antigens and the immune response is restricted to inappropriately-expressed fetal or normal antigens, or to 'neo' antigens, some of which may be expressed as a consequence of the malignant process [7,8,141. The weakness of the antibody response in the present study was not entirely surprising, given the syngeneic nature of the immunogens, the low inoculate level and relatively short immunization schedule, and the absence of adjuvant.…”

Section: Discussionmentioning

confidence: 99%

Production and evaluation of syngeneic antibodies showing specificity for the A15 A5 transplantable rat glioma

Clarke

Pilkington

1995

Neuropathology Appl Neurobio

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Antibodies have been raised which show specificity for the A15 A5 transplantable rat glioma model. Viable, irradiated, colcemid-treated and microwave-fixed A15 A5 cells were each used to hyperimmunize BDIX rats by subcutaneous inoculation, while viable cells were also injected intracerebrally into syngeneic animals. Specific anti-A15 A5 antibodies were detected in serum by a modified enzyme-linked immunoabsorbent assay (ELISA). Immunoperoxidase and immunofluorescence cytochemistry revealed the presence of antigen in paraffin-wax and frozen sections, respectively, of A15 A5 gliomas. The different methods of immunogen denaturation were found to affect the degree of immunological potency of the cells. This study has shown that A15 A5 cells are immunogenic in a syngeneic host. Intracerebrally transplanted, viable cells produced antibodies of higher titre than subcutaneously-injected, denatured cells. The anti-A15 A5 antisera may be used for detection of neoplastic rat astrocytes using immunocytochemistry. These antibodies will prove to be of value in discriminating between neoplastic and normal cells at the tumor/brain interface.

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Coexpression of stem cell factor and its receptor c-Kit in human malignant glioma cell lines

et al. 1995

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Stem cell factor (SCF), a hematopoietic growth factor, is the ligand of the tyrosine kinase receptor encoded by the c-kit proto-oncogene. Beside the important role of this receptor-ligand complex in hematopoiesis, gametogenesis and melanogenesis, SCF and its receptor have been shown to be expressed in the brain. We have studied the expression of SCF and c-kit in 20 human malignant glioma cell lines at the mRNA as well as at the protein level. In addition, recombinant human (rh) SCF was tested in [3H]thymidine uptake assays for a mitogenic effect on these cells. SCF and c-Kit proteins were detected in the cytoplasm of glioma cells by alkaline phosphatase-monoclonal anti-alkaline phosphatase immunostaining and Western blot analysis. However, neither SCF nor c-Kit were seen on the cell surface by flow cytometry. Furthermore, none of the proliferation assays showed a mitogenic effect for exogenously added rhSCF. Blocking studies using an anti-SCF antibody failed to demonstrate modulating effects on the growth of selected cell lines. These results suggest that SCF and c-Kit may mediate non-proliferative signals or may employ intracellular mechanisms for autocrine growth regulation of glioma cells.

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Antigenic phenotypes of cultured malignant astrocytomas: Identification of lineage-consistent, lineage-independent and putative tumor-restricted antigenic expression

Cited by 7 publications

References 38 publications

Expression of the extraneuronal monoamine transporter (uptake2) in human glioma cells

Expression of the extraneuronal monoamine transporter (uptake2) in human glioma cells

Production and evaluation of syngeneic antibodies showing specificity for the A15 A5 transplantable rat glioma

Coexpression of stem cell factor and its receptor c-Kit in human malignant glioma cell lines

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