Antigenic profiles of endothelial and hemopoietic lineages in murine intraembryonic hemogenic sites

García‐Porrero, Juan A.; Manaia, Alexandra; Jimeno, Jaime; Lasky, Laurence L; Dieterlen‐Lièvre, Françoise; Godin, Isabelle

doi:10.1016/s0145-305x(98)00006-8

Cited by 69 publications

(57 citation statements)

References 45 publications

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“…These markers also label endothelial cells and reversibly, endothelial markers are often expressed by primitive hematopoietic precursors. The hematopoietic nature of aortic clusters could only be demonstrated through the lack of expression of von Willebrand factor, an endothelial marker expressed from the earliest stages of blood vessel development (Garcia-Porrero et al, 1998). These observations extended to the mouse embryo a conclusion already drawn for the aortic clusters in human embryos (Tavian et al, 1996).…”

Section: Where Are Intra-embryonic Hscs Generated?mentioning

confidence: 76%

“…To assess whether these intra-arterial clusters were hematopoietic, we analysed their phenotype based on the expression of immature hematopoietic cell surface markers such as CD34 or AA4.1 (Figure 2A) (Garcia-Porrero et al, 1998;Petrenko et al, 1999). These markers also label endothelial cells and reversibly, endothelial markers are often expressed by primitive hematopoietic precursors.…”

Section: Where Are Intra-embryonic Hscs Generated?mentioning

confidence: 99%

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Of birds and mice: hematopoietic stem cell development

Godin

Cumano²

2005

Int. J. Dev. Biol.

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For many years it has been assumed that the ontogeny of the mammalian hematopoietic system involves sequential transfers of hematopoietic stem cells (HSCs) generated in the yolk sac blood islands, to successive hematopoietic organs as these become active in the embryo (fetal liver, thymus, spleen and eventually bone marrow). Very little was known about early events related to hematopoiesis that could take place during the 4.5 day gap separating the appearance of the yolk sac blood islands and the stage of a fully active fetal liver. Experiments performed in birds documented that the yolk sac only produce erythro-myeloid precursors that become extinct after the emergence of a second wave of intra-embryonic HSCs from the region neighbouring the dorsal aorta. The experimental approaches undertaken over the last ten years in the murine model, which are reviewed here, led to the conclusion that the rules governing avian hematopoietic development basically apply to higher vertebrates.

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Section: Where Are Intra-embryonic Hscs Generated?mentioning

confidence: 76%

Section: Where Are Intra-embryonic Hscs Generated?mentioning

confidence: 99%

Of birds and mice: hematopoietic stem cell development

Godin

Cumano²

2005

Int. J. Dev. Biol.

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“…During midgestation, cell aggregates (called hematopoietic clusters) that are intimately associated with endothelium are observed in the large vessels of the mouse embryo [aorta, UA and VA (Garcia-Porrero et al, 1995;Garcia-Porrero et al, 1998;Jaffredo et al, 2005)] and in the yolk sac (Ferkowicz et al, 2003). The yolk sac cluster cells (E8.25) are CD41 + definitive hematopoietic progenitors (Ferkowicz et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

“…Hematopoietic clusters have been examined in a variety of vertebrate embryos (Jaffredo et al, 2005) and characterized by immuno-and histochemical staining of transversal sections (Garcia-Porrero et al, 1995;Garcia-Porrero et al, 1998;Jaffredo et al, 1998;Marshall and Thrasher, 2001;North et al, 1999;North et al, 2002;Taoudi and Medvinsky, 2007;Tavian et al, 1996). A reconstruction of an immunostained 5-week human embryonic aorta made from 72 transverse sections with a thickness of 5 m each (one or two somite pairs in preumbilical region) shows 831 CD34 + clustered cells (Tavian et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

Three-dimensional cartography of hematopoietic clusters in the vasculature of whole mouse embryos

2010

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SUMMARYHematopoietic cell clusters in the aorta of vertebrate embryos play a pivotal role in the formation of the adult blood system. Despite their importance, hematopoietic clusters have not been systematically quantitated or mapped because of technical limitations posed by the opaqueness of whole mouse embryos. Here, we combine an approach to make whole mouse embryos transparent, with multicolor marking, to allow observation of hematopoietic clusters using high-resolution 3-dimensional confocal microscopy. Our method provides the first complete map and temporal quantitation of all hematopoietic clusters in the mouse embryonic vasculature. We show that clusters peak in number at embryonic day 10.5, localize to specific vascular subregions and are heterogeneous, indicating a basal endothelial to non-basal (outer cluster) hematopoietic cell transition. Clusters enriched with the c-Kit-cell population contain functional hematopoietic progenitors and stem cells. Thus, three-dimensional cartography of transparent mouse embryos provides novel insight into the vascular subregions instrumental in hematopoietic progenitor/stem cell development, and represents an important technological advancement for comprehensive in situ hematopoietic cluster analysis.

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“…Histological detection of these hemopoietic clusters was investigated previously. However, the investigations analyzed the expression of molecules which are not restricted to HC but shared with other cell lineages, either the endothelial lineage (CD31, CD34) or with more differentiated hematopoietic cells (CD45) (Garcia-Porrero et al, 1998;Wood et al, 1997;North et al, 1999;de Bruijn et al, 2000;Manaia et al, 2000;Tavian et al, 1996). Furthermore, in both chicken and mouse models, cell sorting on the basis of αIIb establish the antibody against αIIb as a sensitive tool to sort out progenitors with multilineage potential.…”

Section: Discussionmentioning

confidence: 99%