Antigenic profiles of human, bovine and canine articular chondrocytes

Giant, Tibor T.; Mikecz, Katalin

doi:10.1007/bf00219212

Cited by 23 publications

(29 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Absorption with these components did not abolish reaction against chondrocytes, suggesting that resented the main type of cells involved in resorption of devitalized xenogeneic cartilage implants (33). Death of the absorbed sera contained cytotoxic antibodies against cellular antigens (22). In comparison with published chondrocytes in our transplants presumably followed formation of antichondrocyte antibody and complement procedures, sensitization of rabbits with transplants of chondrocytes was quite efficient, because serum with activation.…”

Section: Electrophoresis and Western Blotting Isolation Of Skin Fibromentioning

confidence: 80%

“…Bifor several months produced antichondrocyte antibodies otinylated goat anti-mouse immunoglobulins served as whose titer was not measurably reduced after absorption secondary antibody (DAKO). Reaction was completed of serum with lymphocytes (22,37). These last observawith extravidin-peroxidase conjugate and diaminobentions encouraged us to study transplants of rat chondrozidine tablets (Sigma).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Transplants of Rat Chondrocytes Evoke Strong Humoral Response against Chondrocyte-Associated Antigen in Rabbits

et al. 2003

View full text Add to dashboard Cite

Rat chondrocytes transplanted intramuscularly in rabbits produced cartilage. In 1-day-old transplants chondrocytes remained viable. After 1 week peripheral chondrocytes of the transplant were dead and the cartilage was surrounded and resorbed by macrophages. In 2-week-old transplants cartilage deteriorated and was invaded by fibroblast-like cells and macrophages. Sera of rabbits that received two or three consecutive transplants of rat chondrocytes with 2-week intervals contained high titer of antichondrocyte cytotoxic antibodies. A part of the cytotoxic activity could be removed by absorption with rat splenocytes. Western blot analysis of lysates from fresh or 24-h cultured chondrocytes with absorbed sera detected antigen with M r of ϳ74 and ϳ23 kDa. Only the latter remained after reduction in 2-mercaptoethanol. In lysates of fibroblasts and endotheliocytes the 23-kDa antigen was not found but the serum reacted with M r 39-kDa antigen. In lysates of thymocytes a weak band corresponding to M r of 35 kDa was present. Serum from rabbits receiving transplants of living chondrocytes followed by chondrocytes suspended in complete Freund's adjuvant contained antibodies directed against components of crude collagenase used for cell isolation. Such antibodies could not be detected in sera of rabbits receiving transplants of living chondrocytes only. Molecular weight of detected antigen differs from that of collagen type II, core of aggrecan, link proteins, and several other macromolecules of cartilage matrix. It could represent either a component of chondrocyte membrane or a membrane-bound substance resistant to enzymes used for isolation. Availability of antibodies against presumably chondrocyte-specific antigen produced during transplant rejection may help to characterize it more precisely and to ascertain whether its presence may influence results of autogenous chondrocyte transplants in humans.

show abstract

Section: Electrophoresis and Western Blotting Isolation Of Skin Fibromentioning

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Transplants of Rat Chondrocytes Evoke Strong Humoral Response against Chondrocyte-Associated Antigen in Rabbits

et al. 2003

View full text Add to dashboard Cite

show abstract

“…unknown. Whether it represents a cartilage tissue-specific antigen(s), the presence of which has already been suggested by other investigators (25)(26)(27), also remains a matter of speculation.…”

Section: Discussionmentioning

confidence: 92%

Natural cell‐mediated cytotoxicity against syngeneic rat chondrocytes originating from different types of cartilage

Radomska

Osiecka²,

Malejczyk³

1989

Immunol Cell Biol

View full text Add to dashboard Cite

Summary Natural anti-chondrocyte cylotoxicity of normal rat splenocytes, peritoneal cells and thymocytes was tested by means of ^'Cr-release assay. Chondrocytes derived from epiphyseal, costal, nasal, and auricular cartilages were used as target cells. In some experiments, erythroleukaemic K-562 cells, known as typical natural killer cell targets, were also used. All types of chondrocytes were lysed equally well by splenocytes. Peritoneal cells exerted a low cytotoxic effect, whilst very low. almost negligible, cytotoxicity was noted with thymocytes. Negative selection with antibodies and complement showed that spleen-derived anti-chondrocyte effector cells arc endowed with surface ganglioside asialo-GMI. A similar result was obtained in parallel experiments with K-562 cells. Moreover, 'cold' target experiments demonstrated that the release of ^'Cr from the labelled chondrocytes could be inhibited by addition of untabclled chondrocytes and K-562 cells.

show abstract

“…The cytotoxic effects of sera (control, nonarthritic, and arthritic mice) and of affinity-purified mouse antibodies to chondroitinase ABC-treated fetal human proteoglycan on mouse and human articular chondrocytes were assayed by a "Cr release assay. Chondrocytes from BALB/c mice (1-2 weeks old) and from fetal human articular cartilage were isolated by incubating cartilage slices with collagenase (4 mg/ml) and DNase I (0.1 mg/ml) in Dulbecco's modified Eagle's medium (DMEM) containing 15% fetal calf serum (FCS), 50 pg/ml of ascorbic acid, and 150 pg/ml of gentamycin (31). Chondrocytes were removed by filtration through 2 layers of a 35-pm nylon filter (Nitex screen; Tetko, Elmford, NY), washed, and 4 x lo6 cellslml were cultured overnight in DMEM containing 15% FCS, ascorbic acid, gentamycin, and 100 pCi/ml of NaZ5lCrO4 in 12-well tissue culture plates (3 ml/well; Flow Laboratories).…”

Section: Weeks Of Immunizationmentioning

confidence: 99%

Immunity to cartilage proteoglycans in balb/c mice with progressive polyarthritis and ankylosing spondylitis induced by injection of human cartilage proteoglycan

Mikecz

Glant

Poole

1987

Arthritis & Rheumatism

159

221

View full text Add to dashboard Cite

Intraperitoneal injection of human fetal cartilage proteoglycan (depleted of chondroitin sulfate) in Freund's complete or incomplete adjuvant induces a chronic erosive polyarthritis and spondylitis in all female BALB/c mice. This occurrence is strain-specific but not haplotype-specific, and it is sex-related. The development of the arthritis is associated with the natural presence of cellular immunity to the immunizing antigen and to chondroitinase ABC-treated mouse cartilage proteoglycan. In addition, relatively more antibody to the immunizing proteoglycan is elicited in arthritic mice, and antibodies are produced that crossreact with native mouse proteoglycan. This combination of immune responses is not observed in mice that do not develop arthritis. Associated with the arthritis is the development of cytotoxicity to mouse chondrocytes and, in some animals, of rheumatoid factor, immune deposits

show abstract

Antigenic profiles of human, bovine and canine articular chondrocytes

Cited by 23 publications

References 30 publications

Transplants of Rat Chondrocytes Evoke Strong Humoral Response against Chondrocyte-Associated Antigen in Rabbits

Transplants of Rat Chondrocytes Evoke Strong Humoral Response against Chondrocyte-Associated Antigen in Rabbits

Natural cell‐mediated cytotoxicity against syngeneic rat chondrocytes originating from different types of cartilage

Immunity to cartilage proteoglycans in balb/c mice with progressive polyarthritis and ankylosing spondylitis induced by injection of human cartilage proteoglycan

Contact Info

Product

Resources

About