Antigenic Properties of Iron Regulated Proteins in Acinetobacter baumannii: An In Silico Approach

Bazmara, Hadise; Rasooli, Iraj; Jahangiri, Abolfazl; Sefid, Fatemeh; Astaneh, Shakiba Darvish Alipour; Payandeh, Zahra

doi:10.1007/s10989-017-9665-6

Cited by 31 publications

(14 citation statements)

References 58 publications

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“…While numerous studies have examined the ability of different bacterial components to induce a range of adaptive immune responses (McConnell and Pachón, 2010; McConnell et al, 2011a,b; García-Quintanilla et al, 2014; Badmasti et al, 2015; Kuolee et al, 2015; Garg et al, 2016; Ainsworth et al, 2017; Bazmara et al, 2017; Pulido et al, 2018; Song et al, 2018), very little work has been done to examine these during the course of infection, due to several confounding factors, including the available animal models, rate of disease progression and severity.…”

Section: Immune Responsesmentioning

confidence: 99%

The Mechanisms of Disease Caused by Acinetobacter baumannii

et al. 2019

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Acinetobacter baumannii is a Gram negative opportunistic pathogen that has demonstrated a significant insurgence in the prevalence of infections over recent decades. With only a limited number of “traditional” virulence factors, the mechanisms underlying the success of this pathogen remain of great interest. Major advances have been made in the tools, reagents, and models to study A. baumannii pathogenesis, and this has resulted in a substantial increase in knowledge. This article provides a comprehensive review of the bacterial virulence factors, the host immune responses, and animal models applicable for the study of this important human pathogen. Collating the most recent evidence characterizing bacterial virulence factors, their cellular targets and genetic regulation, we have encompassed numerous aspects important to the success of this pathogen, including membrane proteins and cell surface adaptations promoting immune evasion, mechanisms for nutrient acquisition and community interactions. The role of innate and adaptive immune responses is reviewed and areas of paucity in our understanding are highlighted. Finally, with the vast expansion of available animal models over recent years, we have evaluated those suitable for use in the study of Acinetobacter disease, discussing their advantages and limitations.

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Section: Immune Responsesmentioning

confidence: 99%

The Mechanisms of Disease Caused by Acinetobacter baumannii

et al. 2019

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“…In this respect, the antigens producing protective active and passive immunization could be assumed as appropriate. To date, a number of in silico, in vitro and in vivo investigations have been conducted to nominate or design protein antigens (Fattahian et al 2011;Bentancor et al 2012;Luo et al 2012;Rahbar et al 2012;Lin et al 2013;Moriel et al 2013;Fajardo Bonin et al 2014;Hosseingholi et al 2014;Badmasti et al 2015;Chiang et al 2015;Huang et al 2015Sefid et al 2015Garg et al 2016;Singh et al 2016;Jahangiri et al 2017, 2018a, b Ni et al 2017Guo et al 2018;Song et al 2018;Bazmara et al 2017), OmpA (Luo et al 2012;Lin et al 2013), OmpW (Huang et al 2015), Bap (Fattahian et al 2011), Omp22 (Huang et al 2016) and FilF (Singh et al 2016) are among protective antigens against this nosocomial pathogen. Amongst the antigens, outer membrane proteins (OMPs) are considerable owing to their roles in antibiotic resistance, adhesion to host cells and pathogenicity of the bacterium in addition to their high immunogenicity (McConnell et al 2013;Fajardo Bonin et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Outer Membrane Protein, Oma87 Prevents Acinetobacter baumannii Infection

Rasooli

Abdolhamidi

Jahangiri

et al. 2020

Int J Pept Res Ther

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Acinetobacter baumannii is one of the most problematic pathogens in clinical settings. Emerging of its antibiotic-resistant strains persuade researchers to find alternative treatment options such as immunization against the notorious nosocomial pathogen. Oma87 has been introduced as an immunogenic outer membrane protein via reverse vaccinology. However, protectivity of A. baumannii Oma87 is not well known. The current research undertakes a study on the immunogenicity of recombinant Oma87 in a murine model. Some physico-chemical properties were assessed via in silico analyses. The corresponding gene was amplified and cloned into pET28a plasmid. The recombinant protein was purified and then was administered to immunize mice. Sera obtained from the immunized mice were assessed with respect to the triggered antibodies. Challenges were performed on actively or passively immunized mice. In silico analyses revealed that this protein is the same as BamA. A high titer of specific antibody was raised against rOma87 even after the first injection. The specific antibody recognized the whole cell of A. baumannii. Both active and passive immunizations confer 100 and 50% protection, respectively against ~ 2 × lethal dose (LD) of A. baumannii in the murine sepsis model. Although none of mice received ~ 5 × LD of A. baumannii survived in passive immunization, 25% of mice challenged with ~ 7 × LD of the bacteria survived and the dead mice exhibited a delayed death. Based on these results, Oma87 is the same as BamA which could be considered as a promising vaccine candidate against A. baumannii in the sepsis model.

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“…The server can be used on its own or in combination with alignment-based. 57 TLRs identify the pathogen and are responsible for inducing innate immunity. Peta-flops-scale supercomputers can be used for modulation, screening, and identification of new lead structures for hTLR4.…”

Section: Rational Vaccine Designmentioning

confidence: 99%

Computational tools for modern vaccine development

Sunita

Sajid

Singh

et al. 2019

Human Vaccines & Immunotherapeutics

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Vaccines play an essential role in controlling the rates of fatality and morbidity. Vaccines not only arrest the beginning of different diseases but also assign a gateway for its elimination and reduce toxicity. This review gives an overview of the possible uses of computational tools for vaccine design. Moreover, we have described the initiatives of utilizing the diverse computational resources by exploring the immunological databases for developing epitope-based vaccines, peptide-based drugs, and other resources of immunotherapeutics. Finally, the applications of multi-graft and multivalent scaffolding, codon optimization and antibodyomics tools in identifying and designing in silico vaccine candidates are described.

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Antigenic Properties of Iron Regulated Proteins in Acinetobacter baumannii: An In Silico Approach

Cited by 31 publications

References 58 publications

The Mechanisms of Disease Caused by Acinetobacter baumannii

The Mechanisms of Disease Caused by Acinetobacter baumannii

Outer Membrane Protein, Oma87 Prevents Acinetobacter baumannii Infection

Computational tools for modern vaccine development

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