Antigenic Properties of Mycoplasma Organisms and Membranes

Abstract

SUMMARYThe antigenic properties of the soluble and membrane-bound proteins of Mycoplasma organisms were studied by a variety of gel-diffusion techniques. The strains tested belonged to several distinct serological groups, showing no cross-reactions. One group consisted of the Mycoplasma Zaidlawii strains and the other of caprine and bovine strains, which were closely related to Mycoplasma mycoides var. mycoides. The avian strains tested were serologically heterogenous. After Mycoplasma membranes were solubilized by sodium dodecyl sulphate (SDS) the antigenic properties of their membrane proteins were analysed. In immunodiffusion tests they showed a serological specificity similar to the soluble cell proteins. This specificity was not found in lipids of M. laidlawii membranes extracted with chloroform + methanol. In gel diffusion tests the hydrophobic protein fraction isolated from M. laidlawii membranes by detergent action and (NHJ,S04 precipitation reacted with antiserum to whole organisms, was antigenic in rabbits and there produced an antiserum which reacted with it, but not with the soluble cell proteins or membrane material solubilized in SDS.

Type Strains of Species of the Order Mycoplasmatales, Including Designation of Neotypes for Mycoplasma mycoides subsp. mycoides, Mycoplasma agalactiae subsp. agalactiae, and Mycoplasma arthritidis

“…Moreover, in the case of a new species or subspecies, so that the original "type" material on which the description was based may be preserved, an authentic culture of the designated type strain should be deposited with one or more permanently established culture collections (Recommendation 9c). Only in a minority of the original descriptions of named species of the order Mycoplasmatales has a type strain been unequivocally designated, although in other instances only a single strain was described, that strain thus becoming the type by monotypy (Rule 9d [2]). These species are listed in Table 1 with their type strains.…”

Electrophoretic Analysis of Cell Proteins of T-Strain Mycoplasmas Isolated from Man

“…In many cases the variations were rather in the quantity of the protein migrating in a certain band. Figure 6 on August 5, 2020 by guest http://jb.asm.org/ Downloaded from logical relationships can better be detected by complement-fixation and immunodiffusion tests (1). The difficulties encountered in obtaining sufficient cell material required for these tests prevent their use with the T-strains.…”

Characterization of the metabolism inhibition antigen of Mycoplasma arthritidis

“…For Mycoplasma mycoides the major antigens are carbohydrate (5,22). In the case of at least three mycoplasmas, Mycoplasma fermentans, Mycoplasma hominis, and M. pulmonis, the major membrane antigens are protein (2,17).…”