Antigenic Relationship Between Human and Simian Rotaviruses

Schoub, Barry D.; Lecatsas, G.; Prozesky, O. W.

doi:10.1099/00222615-10-1-1

Cited by 32 publications

(17 citation statements)

References 24 publications

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“…The simian rotavirus SA11 has been considered a good surrogate antigen for human rotavirus field strains [Schoub et al,1977;Kapikian and Chanock, 1996] and has been previously applied in immunoblotting assays to analyze the polypeptides of rotavirus recognized by serum antibodies detected in children [Ushijima et al, 1989;Brü ssow et al 1990;Andrade et al, 1996]. The suitability of this viral strain for use as antigen in ELISA tests, Western blots, and RIP assays for analyzing antibody responses to rotavirus was previously assessed in our laboratory in comparison with the cell-culture-adapted human rotavirus strains Wa and Ito.…”

Section: Discussionmentioning

confidence: 99%

Viral proteins VP2, VP6, and NSP2 are strongly precipitated by serum and fecal antibodies from children with rotavirus symptomatic infection

et al. 1998

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Rotavirus-specific IgA has been correlated with immune protection against rotavirus reinfection and symptomatic disease. Systemic and mucosal antibody responses were determined by an enzyme-linked immunosorbent assay in 11 infants with severe rotavirus gastroenteritis. Geometric mean titers of antirotavirus serum IgG and IgA antibodies were significantly higher during the convalescence of the disease (P < 0.001 vs. acute-phase titers). Rotavirus-specific fecal sIgA antibodies increased 4 times during the convalescence in 9 (81.8%) children (P < 0.001). The serum IgG and IgA antibody and fecal sIgA antibody responses to individual rotavirus polypeptides were characterized by radioimmunoprecipitation assay (RIPA) using Staphylococcus aureus protein A and the lectin jacalin to precipitate IgG- and IgA-immune complexes, respectively. The main IgG response was directed toward the structural viral proteins VP2, VP4, and VP6 and toward the nonstructural protein NSP2. Serum IgA reactivity was detected by RIPA in all serum samples, with major responses to VP2, VP6, and NSP2. Interestingly, fecal sIgA in convalescent samples reacted strongly toward NSP2 and VP6. These data reinforce the antigenic importance of rotaviral proteins other than VP4 and VP7, such as VP2, VP6, and NSP2, as main targets in the immune response to rotavirus.

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Section: Discussionmentioning

confidence: 99%

Viral proteins VP2, VP6, and NSP2 are strongly precipitated by serum and fecal antibodies from children with rotavirus symptomatic infection

et al. 1998

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“…Serum, colostrum and milk specimens were absorbed overnight with vervet-monkey cells at 4°C. Antirotavirus antibodies were assayed by immunofluorescence with antigen that consisted of vervet-monkey kidney cells infected with simian rotavirus (SA11) (Schoub, Lecatsas and Prozesky, 19770). The teflon-coated slides containing SAll antigen were incubated with the serum or whey specimens for 1 h at 37"C, before treatment with fluorescein-conjugated goat-antihuman immunoglobulin or antihuman IgA serum (Behring Diagnostics).…”

Section: Methodsmentioning

confidence: 99%

The Role of Breast-Feeding in the Prevention of Rotavirus Infection

Schoub¹,

Prozesky

Lecatsas

et al. 1978

Journal of Medical Microbiology

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“…This group reaction is associated with the inner eapsid layer (41,75,147). They are, however, distinguishable from each other by serum neutralization, though there is some cross-reaction (112,128,147). The rot~viruses thus form a coherent, interrelated group.…”

Section: Antigenic Relationshipsmentioning

confidence: 98%