Antigenic relationships among the rickettsiae of the spotted fever and typhus groups

Vishwanath, Suryanarayanan

doi:10.1111/j.1574-6968.1991.tb04783.x

Cited by 52 publications

(36 citation statements)

References 18 publications

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“…Ladder bands of SFG and TG rickettsiae demonstrated on Western immunoblots have been shown to be polysaccharide in nature (1,32). In the present study, we also showed that ladder bands of R. japonica and R. typhi are polysaccharide (Fig.…”

Section: Discussionsupporting

confidence: 68%

See 1 more Smart Citation

Cross‐Reactivity of Rickettsia japonica and Rickettsia typhi Demonstrated by Immunofluorescence and Western Immunoblotting

Uchiyama

Zhao

Yan-sheng

et al. 1995

Microbiology and Immunology

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Cross-reactivity between Rickettsia japonica and R. typhi was observed by immunofluorescence tests using sera from patients with Oriental spotted fever (OSF), from whom the causative agent was isolated and identified as R. japonica. Western immunoblotting with these sera revealed that only the 120-kilodalton surface polypeptide, i.e., rickettsial outer membrane protein (rOmp) B, has a common antigenicity with the 105-kilodalton surface polypeptide of R. typhi. In some cases, antibodies specifically reactive with R. typhi were detected in acute-phase sera followed by a significant rise in titers, possibly because of an anamnestic response to a previous infection with an R. typhi-like agent; the sera retained reactivity to R. typhi even after absorption by a homologous strain. A lipopolysaccharide (LPS)-like antigen of R. typhi was found to be reactive with some sera of OSF patients. The ladder bands on Western immunoblot of rickettsial organisms were confirmed to be polysaccharide in nature, which was demonstrated by comparing them with the pattern of silver-stained gel of proteinase K-treated rickettsial specimens after sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

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Section: Discussionsupporting

confidence: 68%

“…It has been reported that the antigenicity of rickettsial LPS is group specific, which has been demonstrated by using monoclonal antibody, antisera against SFG and TG rickettsiae, and convalescent-phase sera from patients with Rocky Mountain spotted fever (3,10,15,32). However, Western immunoblotting in the present study (Fig.…”

Section: Discussioncontrasting

confidence: 46%

Cross‐Reactivity of Rickettsia japonica and Rickettsia typhi Demonstrated by Immunofluorescence and Western Immunoblotting

Uchiyama

Zhao

Yan-sheng

et al. 1995

Microbiology and Immunology

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“…The SFG rickettsiae consist of more than 20 named species, which are transmitted by tick bite except for R. akari (mite-borne) and R. felis (flea-borne). Antibodies to LPS antigens cross-react among organisms within the same biogroup, but do not crossreact between the two groups (Vishwanath, 1991). There are two major outer membrane proteins in Rickettsia OmpA (Sca 0) and OmpB (Sca5).…”

Section: Etiologic Agentsmentioning

confidence: 99%

Rickettsia and Rickettsial Diseases

Yu¹,

Walker²

2012

Bioterrorism

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“…Rickettsia prowazekii causes epidemic louse-borne typhus fever, one of the most important infectious diseases of the last 500 years from the perspective of its historic impact (Zinsser, 1935;Traub et al, 1978). These obligately intracellular bacteria are closely related genetically and share many antigenic and biologic characteristics (Vishwanath, 1991;Roux and Raoult, 1995;Roux et al, 1997;Stothard and Fuerst, 1995). Their shared antigens include the group-specific lipopolysaccharide and many epitopes of the major immunodominant surface protein.…”

mentioning

confidence: 99%

Establishment of a Novel Endothelial Target Mouse Model of a Typhus Group Rickettsiosis: Evidence for Critical Roles for Gamma Interferon and CD8 T Lymphocytes

Walker

Popov

Feng

2000

Laboratory Investigation

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SUMMARY:A mouse model of typhus rickettsiosis that reproduces the hematogenous dissemination to the critical target organs, including brain, lungs, heart, and kidneys, primary endothelial and, to a lesser degree, macrophage intracellular rickettsial infection, and typical vascular-based lesions of louse-borne typhus and murine typhus was established. Intravenous inoculation of C3H/HeN mice with Rickettsia typhi caused disease with a duration of the incubation period and mortality rate that were dependent on the infective dose of rickettsiae. Lethal infection was associated with high concentrations of R. typhi in the lungs and brain, despite a brisker humoral immune response to the rickettsiae than in the sublethal infection. Gamma interferon and CD8 T lymphocytes were demonstrated to be crucial to clearance of the rickettsiae and recovery from infection in experiments in which specific monoclonal antibodies were administered to deplete these components. Death of animals depleted of gamma interferon or CD8 T lymphocytes was associated with overwhelming rickettsial infection demonstrated by titers of infectious rickettsiae and by immunohistochemistry. An effective antirickettsial immune response was associated with elevated serum concentrations of IL-12 on Day 5 and increased secretion of IL-12 by concanavalin-A-stimulated spleen cells on Day 5. Evidence for transient suppression of the immune response consisted of marked reduction in the secretion of IL-2 and IL-12 by concanavalin-A-stimulated spleen cells on Days 10 and 15. This model offers excellent opportunities for study of attenuation and pathogenetic mechanisms of typhus rickettsiae, which are established biologic weapons of potential use in bioterrorism. (Lab Invest 2000, 80:1361-1372.

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Antigenic relationships among the rickettsiae of the spotted fever and typhus groups

Cited by 52 publications

References 18 publications

Cross‐Reactivity of Rickettsia japonica and Rickettsia typhi Demonstrated by Immunofluorescence and Western Immunoblotting

Cross‐Reactivity of Rickettsia japonica and Rickettsia typhi Demonstrated by Immunofluorescence and Western Immunoblotting

Rickettsia and Rickettsial Diseases

Establishment of a Novel Endothelial Target Mouse Model of a Typhus Group Rickettsiosis: Evidence for Critical Roles for Gamma Interferon and CD8 T Lymphocytes

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