Antigenic relationships between rotaviruses from different species as studied by neutralization and immunofluorescence

Sato, Kunihiko; Inaba, Yuji; Miura, Yasuo; Tokuhisa, S.; Matumoto, Minoru

doi:10.1007/bf01341726

Cited by 58 publications

(36 citation statements)

References 22 publications

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“…The cross-reactions between PP-1 and the porcine virus suggests that rotaviruses from cattle and pigs may not be antigenically distinct as suggested by Thouless et al (1977) and Sato et al (1982). Some other rotaviruses from different animal species cross-react antigenically in typespecific tests either in a one-or two-way fashion, e.g.…”

Section: Discussionmentioning

confidence: 97%

“…For example, rotaviruses which share a common group antigen from different animal species can be distinguished by neutralization tests (Thouless et al, 1977;Estes & Graham, 1980;Sato et al, 1982), suggesting some degree of species specificity. However, inter-species transmissions (for review, see McNulty, 1978), cross-protection between rotaviruses or by antibody-containing fluids from different species (Wyatt et aL, 1979;Bartz et al, 1980;Bridget & Brown, 1981) and antigenic cross-relationships between some rotaviruses from different animal species (Bastardo et al, 1981 ;Gaul et al, 1982;Hoshino et al, 1982Hoshino et al, , 1983 indicate that rotaviruses may not all be species-specific.…”

Section: Introductionmentioning

confidence: 99%

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Antigenic and Pathogenic Relationships of Three Bovine Rotaviruses and a Porcine Rotavirus

Bridger¹,

Brown²

1984

Journal of General Virology

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SUMMARYThe antigenic relationships between three bovine rotaviruses, UK, CP-1 and PP-1, and a porcine rotavirus were investigated; their pathogenicity for piglets was also assessed. After propagation and cloning in cell culture, the four viruses were used to produce convalescent and hyperimmune antisera in gnotobiotic animals. For the UK and PP-1 viruses, reciprocal virus neutralization tests gave ratios of homologous to heterologous titres in the range 57 to > 2300. Taking a 20-fold difference in titre to be the criterion of heterogeneity, these two viruses could be regarded as separate serotypes. The third bovine virus, CP-1, was identical to PP-1 in reciprocal neutralization tests but gave a one-way cross-reaction with UK convalescent sera, i.e. it appeared to be intermediate between the two serotypes. UK and CP-1 differed antigenically from the porcine virus as shown by .homologous:heterologous neutralization titre ratios of 30 to 12 600. The bovine virus, PP-1, however, had a closer relationship with the porcine virus, the homologous : heterologous ratios ranging from 4 to 110. Oral inoculation of piglets with the bovine viruses revealed differences in their pathogenicity. Isolates UK and CP-1 caused no clinical disease and could not be passaged. However, on the first and second passages in pigs, the bovine virus PP-1 and the porcine virus produced clinical disease, excretion of virus in the faeces and seroconversion. The results suggest that PP-1 may be a natural hybrid between a bovine and a porcine rotavirus.

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Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Antigenic and Pathogenic Relationships of Three Bovine Rotaviruses and a Porcine Rotavirus

Bridger¹,

Brown²

1984

Journal of General Virology

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“…NT was carried out in tube cell cultures essentially as previously described (Sato et al, 1982). Rotavirus of serotype 1 (Wa strain), serotype 2 (DS-1 strain) and serotype 3 (SA-11) were activated with 10 p.g/ml trypsin (14900 units/mg) at 37 °C for 30 min before use.…”

Section: Seramentioning

confidence: 99%

Serum Antibody Responses to Individual Viral Polypeptides in Human Rotavirus Infections

Svensson¹,

Sheshberadaran²,

Vene³

et al. 1987

Journal of General Virology

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SUMMARYA radioimmunoprecipitation assay (RIPA) was used to study the serum antibody responses to individual polypeptides that developed after infection with viruses from human rotavirus subgroups I and II. Paired sera from eight children (1 to 8.5 years of age) were used in the study. Although all of the eight acute sera were negative by the complement fixation test, four of them were positive by RIPA, indicating a previous infection by rotavirus. A significant difference in the number of polypeptides immunoprecipitated was seen among the convalescent sera. The number of polypeptides immunoprecipitated was found to be related to previous infection experience. At most, ten different polypeptides were immunoprecipitated: seven structural polypeptides VPl to VP7 and three non-structural polypeptides, NSl, NS 2 and NS3. No sera immunoprecipitated VP8 or VP9. Acute sera positive by RIPA immunoprecipitated up to five polypeptides, VP1, VP2, VP3, VP4 and VP6. One of the non-structural proteins (NS2) was found to be particularly immunogenic, since antibodies to this polypeptide were detected in several convalescent sera. Among the structural proteins VP2 and VP6 were found to be the two immunodominant polypeptides which were recognized by all convalescent sera. Only three convalescent sera immunoprecipitated VP7, the major type-specific antigen responsible for inducing neutralizing antibodies. Three of four originally seronegative children with no reactivity in the convalescent sera to VP7 developed neutralizing antibodies to a single serotype. One child developed antibodies to two serotypes.

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“…YO, MO, ITO, Nemoto, P2 and S3, isolated from Japan [Kutsuzawa et al, 1982;Sato et al, 1982;Urasawa et al, 1982]; P and M from USA; Pz, RV from Australia [Wyatt et al, 1983]; and AK-35 from Kenya [Urasawa et al, 1987], several other G3 isolates from humans showed closeness to animal G3 strains, especially to feline and canine rotaviruses. For example Japanese strains, AU-1 and AU228 showed close homology to a feline G3 strain FRV-1 [Nakagomi et al, 1987;Nakagomi and Nakagomi, 1989], while HCR3 strain from Philadelphia USA, to feline FRV64 and canine strains CU-1 and K9 [Nakagomi et al, 2000].…”

Section: Discussionmentioning

confidence: 97%

Unexpected detection of simian SA11—human reassortant strains of rotavirus G3P[8] genotype from diarrhea epidemic among tribal children of Western India

Awachat

Kelkar

2005

Journal of Medical Virology

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This investigation is in continuation with the earlier studies on diarrhea epidemic due to rotavirus, that occurred at Jawhar western India, among tribal children during Dec 2000-Jan 2001 published by National Institute of Virology, Pune. Three rotavirus strains were isolated in cell culture from fecal specimens of affected children. Monoclonal antibody ELISA and RT-PCR typed the isolates as G3P [8]. Two isolates agglutinated guinea pig erythrocytes thereby indicating their animal origin. Antiserum against simian G3, SA11 neutralized both the isolates largely than antiserum against human G3 (YO). Nucleotide sequencing of VP7 gene, nested PCR product of three isolates, and two original fecal specimens showed 100% identity with both simian G3, SA11 prototype and ROSVP7, SA11 strains whereas, lower identity (82%) with human G3 (YO) strain. Partial sequence analysis of the VP4 gene of fecal specimen FS-006964 & isolate I-006964 showed 99% identity with G1P[8] strain, while 80% identity with simian G3P[2], SA11 strain. Thus, the strains appeared to be reassortants between human and simian origin. Serological studies further supported the identity of the causative agent as simian G3, SA11 like strains. Neutralizing antibody titers at very low level against simian G3, SA11 as well as human G3 (YO) strains among the population at Jawhar suggested an overall lack of immunity against the virus. Seroconversion against simian G3, SA11 was shown by six out of seven child patients. Thus, simian G3, SA11 like strains are claimed for the first time as etiological agents of diarrhea in humans.

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Antigenic relationships between rotaviruses from different species as studied by neutralization and immunofluorescence

Cited by 58 publications

References 22 publications

Antigenic and Pathogenic Relationships of Three Bovine Rotaviruses and a Porcine Rotavirus

Antigenic and Pathogenic Relationships of Three Bovine Rotaviruses and a Porcine Rotavirus

Serum Antibody Responses to Individual Viral Polypeptides in Human Rotavirus Infections

Unexpected detection of simian SA11—human reassortant strains of rotavirus G3P[8] genotype from diarrhea epidemic among tribal children of Western India

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