Antigenic relationships of selected strains of infectious pancreatic necrosis virus and European eel virus

Abstract

An antigenic comparison of ten strains of infectious pancreatic necrosis virus (IPNV) and a single strain of European eel virus (EEV) revealed the presence of three distinct groups separable by cross-neutralization. The 1/r value calculated from the formula/•= \ /•] x ;-2 (Archetti & Horsfall 1950) was used to divide the virus strains into: group I, which included all six U.S.A.

Gill lamellar pillar cell necrosis, a new birnavirus disease in Japanese eels

“…It had been reported (Sano 1976, Cattric & Chastel 1980, Bucke 1981, Okamoto et al 1983, Chi et al 1991 that birnavirus groups related to Ab and Sp serotypes of IPNV were isolated from European eels which might not have encountered salmonid fishes infected with IPNV, but any pathological effects were unclear because of a lack of histological examinations in these previous studies. In the present study, a birnavirus of PCNV was isolated from Japanese eels that had never encountered salmonid fishes or European eels in Japan, and was revealed to be more closely related antigenically to the Sp rather than to the Ab serotype.…”

“…and the corresponding polyclonal rabbit antisera. An anti-PCNV serum was prepared following to Okamoto et al (1983) using a Japanese white rabbit (12 wk of age). Neutralizing titres were reciprocal of the antiserum dilution that protected 50% of the inoculated wells against the virus 100 TCID,,.…”

Identification of Additional Quantitative Trait Loci (QTL) Responsible for Susceptibility to Infectious Pancreatic Necrosis Virus in Rainbow Trout

“…The IPNV was made to pass 20 times through RTG-2 cells (Wolf and Quimby, 1962). Virus propagation techniques using the RTG-2 cell line were described by Okamoto et al (1983Okamoto et al ( , 1984.…”

“…IPN virus challenge: disease resistance tests and phenotypic measurements Tests of IPNV resistance were conducted for the F 1 hybrid family 94-N515-K560 (n = 50) and the backcross families 97-K2-2 (n = 100) and 97-L (n = 99) according to the method of Okamoto et al (1983). For each test of artificially induced infection, fry weighing approximately 0.15 g were held in a beaker filled with 1000 mlL of aerated water at 15∞C.…”

Comparison of RNAs and Polypeptides of Infectious Pancreatic Necrosis Virus Isolates from Eel and Rainbow Trout

“…A summary of all these isolates (prefixed T for trout and E for eel), their location, code and sampling dates is shown in Table 1. According to the relative degree of cross-neutralization by rabbit antisera, at least three serotypes have been identified (MacDonald & Gower, 1981 ;Hedrick & Okamoto, 1982;Okamoto et al, 1983). Hill & Way (1983) examined 175 virus isolates for cross-neutralization by rabbit antisera and demonstrated six additional serotypes which, together with the three serotypes originally described, constitute (1985) have reported that IPNV strains AB and EVE were isolated from eels in 1981 to 1983 and VR-299 was isolated from trout in Taiwan, our 13 isolates of IPNV were compared with the selected serotypes, AB, SP and VR o 299.…”