Antigenic specificity of a monovalent versus polyvalent MOMP based Chlamydia pecorum vaccine in koalas (Phascolarctos cinereus)

Kollipara, Avinash; Wan, Charles; Rawlinson, Galit; Brumm, Jacqui; Nilsson, Kjell; Beagley, Kenneth W.

doi:10.1016/j.vaccine.2012.12.057

Cited by 34 publications

(40 citation statements)

References 24 publications

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“…[32] were purified according to the published protocol of with modifications as follows: (A) cell lysis were performed by sonication on ice with addition of lysozyme; (B) after binding of His-MOMP proteins to the Ni-NTA beads (Qiagen), washes and elution were performed on a gravity column to prevent contamination of the eluate with affinity beads. A schematic of the MOMP-A,—F, and—G proteins used in the vaccine is provided in S1 Fig.…”

Section: Methodsmentioning

confidence: 99%

Immunization of a wild koala population with a recombinant Chlamydia pecorum Major Outer Membrane Protein (MOMP) or Polymorphic Membrane Protein (PMP) based vaccine: New insights into immune response, protection and clearance

et al. 2017

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We assessed the effects of two different single-dose anti-Chlamydia pecorum (C. pecorum) vaccines (containing either Major Outer Membrane Protein (3MOMP) or Polymorphic Membrane Protein (Pmp) as antigens) on the immune response of a group of wild koalas. Both vaccines elicited a systemic humoral response as seen by the production of anti-chlamydial IgG antibodies in more than 90% of vaccinated koalas. A mucosal immune response was also observed, with an increase in Chlamydia-specific mucosal IgG and/or IgA antibodies in some koalas post-vaccination. Both vaccines elicited a cell-mediated immune response as measured by the production of the cytokines IFN-γ and IL-17 post-vaccination. To determine the level of protection provided by the vaccines under natural conditions we assessed C. pecorum infection loads and chlamydial disease status of all vaccinated koalas pre- and post-vaccination, compared to a non-vaccinated cohort from the same habitat. The MOMP vaccinated koalas that were infected on the day of vaccination showed significant clearance of their infection at 6 months post-vaccination. In contrast, the number of new infections in the PMP vaccine was similar to the control group, with some koalas progressing to disease. Genotyping of the ompA gene from the C. pecorum strains infecting the vaccinated animals, identified genetic variants of ompA-F genotype and a new genotype ompA-O. We found that those animals that were the least well protected became infected with strains of C. pecorum not covered by the vaccine. In conclusion, a single dose vaccine formulated with either recombinant PmpG or MOMP can elicit both cell-mediated and humoral (systemic and mucosal) immune responses, with the MOMP vaccine showing clearance of infection in all infected koalas. Although the capability of our vaccines to stimulate an adaptive response and be protective needs to be fully evaluated, this work illustrates the necessity to combine epitopes most relevant to a large panel of variable strains with an efficient adjuvant.

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Section: Methodsmentioning

confidence: 99%

Immunization of a wild koala population with a recombinant Chlamydia pecorum Major Outer Membrane Protein (MOMP) or Polymorphic Membrane Protein (PMP) based vaccine: New insights into immune response, protection and clearance

et al. 2017

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“…In the most recent study, we evaluated the immunogenicity of a vaccine consisting of either monovalent or polyvalent MOMPs [18]. Animals immunized with individual MOMPs developed strong antibody and lymphocyte proliferation responses to both homologous as well as heterologous MOMP proteins.…”

Section: Introductionmentioning

confidence: 99%

“…pecorum vaccine that could protect against a range of strains. A promising aspect of our most recent trials [17,18] was the cross-reactivity of MOMP antibody responses from vaccinated healthy and diseased koalas, giving hope for the generation of a MOMP-based vaccine that will offer wide cross-protection against the variety of genetically distinct C . pecorum strains circulating in wild koala populations.…”

Section: Introductionmentioning

confidence: 99%

Vaccination of Koalas with a Recombinant Chlamydia pecorum Major Outer Membrane Protein Induces Antibodies of Different Specificity Compared to Those Following a Natural Live Infection

Kollipara

Beagley

2013

PLoS ONE

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Chlamydial infection in koalas is common across the east coast of Australia and causes significant morbidity, infertility and mortality. An effective vaccine to prevent the adverse consequences of chlamydial infections in koalas (particularly blindness and infertility in females) would provide an important management tool to prevent further population decline of this species. An important step towards developing a vaccine in koalas is to understand the host immune response to chlamydial infection. In this study, we used the Pepscan methodology to identify B cell epitopes across the Major Outer Membrane Protein (MOMP) of four C . pecorum strains/genotypes that are recognized, either following (a) natural live infection or (b) administration of a recombinant MOMP vaccine. Plasma antibodies from the koalas naturally infected with a C . pecorum G genotype strain recognised the epitopes located in the variable domain (VD) four of MOMP G and also VD4 of MOMP H. By comparison, plasma antibodies from an animal infected with a C . pecorum F genotype strain recognised epitopes in VD1, 2 and 4 of MOMP F, but not from other genotype MOMPs. When Chlamydia-free koalas were immunised with recombinant MOMP protein they produced antibodies not only against epitopes in the VDs but also in conserved domains of MOMP. Naturally infected koalas immunised with recombinant MOMP protein also produced antibodies against epitopes in the conserved domains. This work paves the way for further refinement of a MOMP-based Chlamydia vaccine that will offer wide cross-protection against the variety of chlamydial infections circulating in wild koala populations.

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“…Further, this prototype vaccine has been shown to be safe, while eliciting a strong immune responses in healthy females, without worsening clinical symptoms in already diseased animals [4]. Importantly, recent trials have also shown cross-reactivity of MOMP antibody responses from vaccinated healthy and diseased koalas [4,6], which implies the potential for wide cross-protection against the variety of genetically distinct C. pecorum strains circulating in wild koala populations [17]. However, for optimum protection of wild koalas a chlamydial vaccine must be effective in both sexes, thus the obvious next step is to assess the vaccine efficacy in the male koala.…”

Section: Introductionmentioning

confidence: 98%

“…membrane [13][14][15][16]. However, to date Chlamydia vaccine studies have focused primarily on female animal models, and indeed initial efforts to develop a MOMP-based vaccine for the koala have been successful in inducing a cell-mediated response, as well as a humoral immune response, in the female koala following subcutaneous (s.c.) vaccination [4][5][6][7]. Further, this prototype vaccine has been shown to be safe, while eliciting a strong immune responses in healthy females, without worsening clinical symptoms in already diseased animals [4].…”

Section: Introductionmentioning

confidence: 99%

Comparison of subcutaneous versus intranasal immunization of male koalas (Phascolarctos cinereus) for induction of mucosal and systemic immunity against Chlamydia pecorum

Waugh

Andrew

Rawlinson³

et al. 2015

Vaccine

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Antigenic specificity of a monovalent versus polyvalent MOMP based Chlamydia pecorum vaccine in koalas (Phascolarctos cinereus)

Cited by 34 publications

References 24 publications

Immunization of a wild koala population with a recombinant Chlamydia pecorum Major Outer Membrane Protein (MOMP) or Polymorphic Membrane Protein (PMP) based vaccine: New insights into immune response, protection and clearance

Immunization of a wild koala population with a recombinant Chlamydia pecorum Major Outer Membrane Protein (MOMP) or Polymorphic Membrane Protein (PMP) based vaccine: New insights into immune response, protection and clearance

Vaccination of Koalas with a Recombinant Chlamydia pecorum Major Outer Membrane Protein Induces Antibodies of Different Specificity Compared to Those Following a Natural Live Infection

Comparison of subcutaneous versus intranasal immunization of male koalas (Phascolarctos cinereus) for induction of mucosal and systemic immunity against Chlamydia pecorum

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