Antigenicity and Cross-Reactions of Bovine Spermatozoa

Hunter, A.G.; Hafs, H. D.

doi:10.1530/jrf.0.0070357

Cited by 44 publications

(21 citation statements)

References 7 publications

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“…Hunter & Hafs (1965) reported that rabbit semen contained nine antigens, seven common to seminal plasma and two specific for spermatozoa. These same authors (Hunter & Hafs, 1964) found that bull spermatozoa possessed at least seven antigens of which five were shared with seminal plasma and one with blood serum.…”

Section: Introductionmentioning

confidence: 93%

Origin of the Antigens in Rabbit Semen Which Induce Antifertility Antibodies

Menge¹,

Protzman²

1967

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Summary. Cattle and guinea-pig antisera against rabbit testis, epididymal spermatozoa and ejaculated semen had high titres of sperm agglutinins and prevented fertilization of ova in rabbits bred with semen treated with these sera. Although cattle antisera produced against seminal plasma from vasectomized rabbits, 9-day-old-rabbit conceptuses and rabbit brain and guinea-pig antiserum to seminal plasma containing epididymal secretions exhibited relatively high titres of sperm agglutinins, none of these sera prevented spermatozoa from fertilizing ova. The antifertility antibodies were removed or inactivated in antisera by absorptions with testis material, epididymal spermatozoa and semen supernatant but not by absorption with either type of seminal plasma.Agar-gel diffusion and immuno-electrophoretic techniques revealed that ejaculated rabbit semen contained at least nine antigens. Seven were common to seminal plasma, two to blood serum and two appeared to be spermatozoa-specific originating in the testis. Antisera to the conceptus material reacted with four to six different antigens in semen of which at least one was not common to the antigens in blood serum.

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Section: Introductionmentioning

confidence: 93%

Origin of the Antigens in Rabbit Semen Which Induce Antifertility Antibodies

Menge¹,

Protzman²

1967

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“…Procedures for the Ouchterlony technique and immuno-electrophoresis were identical to those described by Hunter & Hafs (1964).…”

Section: Introductionmentioning

confidence: 99%

Characterization and Isolation of a Sperm-Coating Antigen From Rabbit Seminal Plasma With Capacity to Block Fertilization

Hunter¹,

Nornes²

1969

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Summary. The relationship between the sperm-coating antigens of rabbit seminal plasma and the phenomenon of decapacitation was studied using agar-gel diffusion, immuno-electrophoresis, chromatography on Sephadex G-200, and polyacrylamide vertical gel electrophoresis.Interpretation of data obtained with these techniques led to the conclusion that a sperm-coating antigen of seminal plasma origin possessed biological activity for blocking fertilization. The sperm-coating antigen was a glycoprotein of approximately 170,000 molecular weight, migrated in an electric field similar to a serum slow \g=b\-globulin and was still present in the seminal fluid of vasectomized males. This sperm\x=req-\ coating antigen was absent from the inactive upper supernatant fluid fraction of seminal plasma after 4 hr of ultracentrifugation at 105,000 g and was present in the active ultracentrifugal pellet.

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“…Ouchterlony precipitation in gel techniques were described previously (Hunter & Hafs, 1964 Hunter & Nornes (1969). BSV suspension (0-5 ml, 6 mg protein) was eluted from the column (IT cm 52 cm) at the rate of 2-6 ml/cm2/hr at room temperature.…”

Section: Introductionmentioning

confidence: 99%