Antigenicity and Polypeptide Composition of Native and Heated Echovirus Type 7 Procapsids

Hasegawa, Ayako; Inouye, Sakae

doi:10.1099/0022-1317-42-1-119

Cited by 5 publications

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References 11 publications

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“…In the case of enterovirus infection, however, the sera of patients often react by CF even with unheated antigens of many different serotypes; thus, CF antibody assays are not useful for determining the serotype of the infecting enterovirus (2, 4, 7, 13, 16, 23). On the other hand, hyperimmune sera to native virions produced in guinea pigs are type specific in CF tests (8,11,26).…”

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confidence: 99%

Production in guinea pigs of antibodies to cross-reactive antigenic determinants of human enteroviruses

Hasegawa¹,

Inouye²

1983

J Clin Microbiol

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We studied the type specificity of the antisera which were produced in guinea pigs by immunization with heat-disrupted virions of human enteroviruses (echovirus types 7 and 11 and coxsackievirus type B5). When guinea pigs were immunized with heated virions (H particles), the antibodies produced reacted type specifically by complement fixation with the homologous H particles. By contrast, when guinea pigs were immunized with disrupted virions prepared by heating in the presence of sodium dodecyl sulfate, antibodies were produced which reacted broadly. Heterotypic complement-fixing antibody responses of humans to enterovirus infections are discussed.

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confidence: 99%

Production in guinea pigs of antibodies to cross-reactive antigenic determinants of human enteroviruses

Hasegawa¹,

Inouye²

1983

J Clin Microbiol

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Type‐Specific and Cross‐Reactive Antigenicity of Capsid Proteins VP1 and VP2 of Echovirus Type 7

Hasegawa

Inouye

1983

Microbiology and Immunology

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After disruption of echovirus type 7 virions with urea and heat, VPI and VP:Z wen> separated by isoclectric focusing in urea-containing sucrose gradients. Antisera to these two polypeptides were produced in guinea pigs, In complement fixation, antiserum to VPI reacted with native and heated virions (N and H antigens, respectively) of homologous virus, and also cross-reacted with heated virions of some other enteroviruses used, Antiserum to VP2 was reactive only with heated virions of homologous and heterologous viruses. Interestingly, the anti-VP2 serum reacted neither with native nor even with heated procapsids (naturally-occurring empty capsids). Antiserum to VPl. but not VP2, showed neutralizing and hemagglutinalion-inhibiting activities. These results suggest that I) both VI'I and VP2 possess cross-r cact ivc antigenic determinants which are exposed on the surface of heated virions, and 2) type-specific determinants of VPI are located on the surface of native vrrions.\\'e have recently succeeded in preparing broad-reactive antisera to human cnteroviruses which are cross-reactive in complement fixation (CF) with heated virions (H antigen) of some heterologous serotypes (II). These antisera were produced by immunization of guinea pigs with virions of echovirus type 7 (E 7), type II (Ell), and coxsackievirus type B5 (CBS) disrupted by relatively mild treatment:heating at 56 (' in the presence of 0.1 % sodium dodecyl sulfate (SDS). Thereafter, the polypeptides of virions which induced cross-reactive antibodies were investigated. Therefore, VPI, VP2, and VP3 of E7 were separated by SDS-polyacrylamide gel electrophoresis (PAGE), and guinea pigs were then immunized with each polypeptide. However, the antisera produced did not react well in CF with heated nor native virions (;'\ antigen) (unpublished d a t a ) . , .. In this study we employed isoclcctric focusing, instead of SDS-PAGE, for separation of VPl and VP2 of E7 after disruption of virions by urea and heat. Ann immunizing guinea pigs with capsid proteins separated in this way, we obtained antisera which possess high antibody activity to homologous and heterologous viruses. The results arc described in this paper.

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Enterovirus Structure and Assembly

Hellen

Wimmer

2014

Human Enterovirus Infections

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Antigenicity and Polypeptide Composition of Native and Heated Echovirus Type 7 Procapsids

Cited by 5 publications

References 11 publications

Production in guinea pigs of antibodies to cross-reactive antigenic determinants of human enteroviruses

Production in guinea pigs of antibodies to cross-reactive antigenic determinants of human enteroviruses

Type‐Specific and Cross‐Reactive Antigenicity of Capsid Proteins VP1 and VP2 of Echovirus Type 7

Enterovirus Structure and Assembly

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