Antigenicity of Trichomonas vaginalis heat-shock proteins in human infections

Davis-Hayman, Sara R.; Shah, Preetam H.; Finley, Richard W.; Meade, John C.; Lushbaugh, William B.

doi:10.1007/s004360050020

Cited by 7 publications

(5 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A strong HSP70 stress response such as that exhibited by all strains tested would enhance Trichomonas survival against the host immune response. In addition, leakage of these HSP70 peptides from trophozoites damaged by immune attack may account for the pronounced in vivo host recognition of trichomonad HSP70s (Davis-Hayman et al 2000). The relationship between the parasite stress response demonstrated in the present study and host HSP70 immune recognition in trichomoniasis, however, is not clear.…”

contrasting

confidence: 48%

“…These HSPs are immunodominant molecules in the host humoral response to Trichomonas infection, and only sera from infected patients recognize some HSPs. The primary HSPs in these experiments, which are both induced during Trichomonas HSR and immunoprecipitated by sera from infected patients, are peptides belonging to the HSP70 class (Davis-Hayman et al 2000).…”

mentioning

confidence: 99%

“…Strain 30 (ATCC 30235) has been described as pathogenic in a subcutaneous mouse assay, and strain 31 (ATCC 30236), isolated from a patient with a mild case of trichomoniasis, has been characterized as mild in the mouse assay (Honigberg et al 1966). Strain PA (a local isolate), strain 41 (ATCC 50141), and strain 42 (ATCC 50142) were isolated from patients with symptomatic metronidazole-resistant trichomoniasis (Muller et al 1988;Davis et al 2000). Strains C4 and C6 were locally isolated from symptomatic patients with metronidazole-susceptible trichomoniasis (Tidwell et al 1994).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Trichomonas vaginalis: analysis of a heat-inducible member of the cytosolic heat-shock-protein 70 multigene family

et al. 2000

Self Cite

View full text Add to dashboard Cite

A 2253-nucleotide (nt) transcript for a Trichomonas vaginalis heat-shock protein 70, TVCHSP70, has been isolated that encodes for a protein of 659 amino acids with a predicted molecular weight of 71.3 kDa. TVCHSP70 has a short (10-nt) 5' untranslated region (UTR), and the 263-nt 3' UTR is the longest reported for a Trichomonas peptide. Amino-acid sequence analysis and phylogenetic comparison identifies TVCHSP70 as a member of the heat-inducible cytoplasmic HSP70 gene family. Southern-blot data indicate that T. vaginalis contains at least four members of the cytoplasmic HSP70 gene family. Members of the TVCHSP70 family are expressed as 2.3-kb transcripts at low levels during 37 degrees C culture, and their expression is significantly up-regulated at 43 degrees C. Slot-blot analysis of seven T. vaginalis clinical isolates demonstrated a 3- to 44-fold up-regulation of TVCHSP70 under conditions of heat shock (43 degrees C) or oxidative stress (500 microm H2O2) as compared with controls (37 degrees C).

show abstract

contrasting

confidence: 48%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Trichomonas vaginalis: analysis of a heat-inducible member of the cytosolic heat-shock-protein 70 multigene family

et al. 2000

Self Cite

View full text Add to dashboard Cite

show abstract

“…It is unlikely to be an anamnestic response since these animals were culture negative for T. foetus prior to parenteral immunization. Similarly, sera from uninfected exposed patients contain antibodies to heat shock proteins of T. vaginalis, which were explained to be natural antibodies (11). However, a 38-kDa antigen was recognized only by antibodies present in sera of infected women, suggesting that active infection is required to elicit a response to this antigen.…”

Section: Discussionmentioning

confidence: 99%

Antibody Responses of Cattle Immunized with the Tf190 Adhesin ofTritrichomonas foetus

Voyich¹,

Ansotegui

Swenson

et al. 2001

Clin Diagn Lab Immunol

View full text Add to dashboard Cite

The antibody response patterns of cattle after subcutaneous and intranasal immunizations with adhesin Tf190 of Tritrichomonas foetus were investigated. Reactions of antibody from cattle parenterally immunized with Tf190 revealed antigen specificity and Tf190 sensitization in the majority of the animals, as determined by Western blotting. The results also demonstrated strong preimmune immunoglobulin G2 (IgG2) binding to T. foetus antigens not seen in IgG1 profiles. Subcutaneous injections of Tf190 resulted in significant (P < 0.05) increases in serum IgG1 and IgG2 titers over time, as determined by parasite specific enzyme-linked immunosorbent assay. Immune sera also significantly inhibited parasite adhesion to mammalian cell lines compared to the level of inhibition obtained with preimmune sera (P < 0.05). Intranasal immunization with Tf190 failed to produce measurable parasite-specific antibody in serum; however, this immunization route did result in significant (P < 0.05) increases in parasite-specific IgA titers in cervical mucus secretions from immunized animals that were more resistant to intravaginal challenge with T. foetus than controls. These results suggest that systemic immunization with Tf190 results in serum antibody production and antiparasitic adhesin antibodies. Additionally, the results of challenge experiments with intranasally immunized animals suggests that Tf190 primes protective immune responses that lead to lower rates of infection among these animals.The sexually transmitted parasitic protozoan Tritrichomonas foetus, the causative agent of bovine trichomoniasis, results in fetal loss due to abortion and increased management costs due to unproductive cows. It is often found on the mucosal surfaces of the female reproductive tract or in the epithelial glands of the penis of the bull (20,21,23,28). There is no approved chemotherapy in the United States, and infected animals are usually culled to control disease in the herd (14). Infected animals can often self-cure, perhaps as a consequence of infection-induced acquired immunity; however, reinfection is common and infected bulls can carry the infection for life, serving as a reservoir for the disease and suggesting that immunity from natural infection rarely protects against challenge. Therefore, the reported herd incidence remains near 10%, despite the availability of a commercial vaccine (15). The present vaccine is also problematic because of its incomplete efficacy, since its use results in only an approximately 30% rate of reduction in the incidence of abortion (15).Advancements in chemotherapy and vaccine development are complicated by a general lack of knowledge of the immune effector functions that contribute to protection and insufficient understanding of the pathogenesis of trichomoniasis. Parasitespecific antibody responses in cattle to natural T. foetus infection (immunoglobulin G1 [IgG1], IgG2, and IgA isotypes) have been demonstrated by a variety of assays and with a variety of parasite antigens (3,9,15,17,25) and in experiment...

show abstract

“…Greater serum and vaginal IgA antibody responses and increased IFN-γ, IL-2, and TNF-α responses have been reported in mice infected with T. vaginalis isolates derived from asymptomatic women compared to those from symptomatic women (Paintlia et al, 2002). Several trichomonad antigens have been identified, including α-actinin (Addis et al, 1999); a phenotypically variable, tandem-repeatcontaining p270 (Musatovova and Alderete, 1999); heatshock proteins (Davis-Hayman et al, 2000); and cysteine proteases, which are involved in cytoadherence (Alderete et al, 1991;Mendoza-Lopez et al, 2000). Monoclonal antibodies have been shown to inhibit motility and adherence to human cells (Hernandez et al, 2004).…”

Section: Trichomonas Vaginalismentioning

confidence: 95%