Antigenotoxic and antioxidant effects of the Mongolian medicinal plant Leptopyrum fumarioides (L): An in vitro study

Boldbaatar, Delgerbat; El‐Seedi, Hesham R.; Findakly, Meriana; Jabri, Safa; Batkhuu, Javzan; Choidash, Battsetseg; Göransson, Ulf; Hellman, Björn

doi:10.1016/j.jep.2014.06.005

Cited by 20 publications

(10 citation statements)

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“…After having used many different ways to analyse the comet data (Andersson et al 2003(Andersson et al , 2007Bergqvist et al 1998;Boldbaatar et al 2014;Demma et al 2009;Durling et al 2009) generated in different studies (see Table 3), we decided to use an approach based on a proportional odds model tailored to continuous outcomes. After consulting a statistician, a model was built and used on our data from the neonicotinoid experiments.…”

Section: Discussionmentioning

confidence: 99%

“…After consulting a statistician, a model was built and used on our data from the neonicotinoid experiments. For comparative purposes, the Durling et al (2009) Tail intensity Cultures None Average medians Two-tailed t test Boldbaatar et al (2014) data were also analysed using a traditional one-way ANOVA on pooled cell data. As far as we know, the proportional odds model tailored to continuous outcomes which we have called Uppsala Comet Data Analysis Strategy (UCDAS) is a completely new approach to analyse comet data.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of Potential DNA-Damaging Effects of Nitenpyram and Imidacloprid in Human U937-Cells Using a New Statistical Approach to Analyse Comet Data

et al. 2019

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Even if the two neonicotinoids nitenpyram and imidacloprid have been considered safe for humans, their potential genotoxicity still remains a matter of discussion. The DNA-damaging effects of these two compounds were therefore evaluated in a lymphoma cell line of human origin (U-937) using the comet assay after 3-h exposure to up to 50 μM, with or without metabolic activation using S9 from human liver. The comet data were analysed using a traditional one-way ANOVA after pooling the data on cellular level, and a new alternative approach we have called Uppsala Comet Data Analysis Strategy (UCDAS). UCDAS is a proportional odds model tailored to continuous outcomes, taking the number of pooled cultures, slides and cells into consideration in the same analysis. To the best of our knowledge, the UCDAS approach when analysing comet data has never been presented before. Without metabolic activation, no increase in DNA damage was observed in the neonicotinoide-exposed cells. Nitenpyram was also without DNA-damaging effects when S9 was added. However, in the presence of S9, imidacloprid was found to increase the level of DNA damage. Whereas the ANOVA showed an increase (P < 0.001) both at 5 and 50 μM, UCDAS showed an increase only at the lowest concentration (P < 0.001). Based on these findings, the two neonicotinoids seem to be of little concern when it comes to their potential genotoxicity. However, since the U-937 cells were rather resistant to our positive controls, they may not be the best cells to use when evaluating potential genotoxicity of chemicals.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Evaluation of Potential DNA-Damaging Effects of Nitenpyram and Imidacloprid in Human U937-Cells Using a New Statistical Approach to Analyse Comet Data

et al. 2019

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“…As a part of our ongoing studies of bioactive constituents from plants commonly used in folk medicine (El-Seedi et al 2012; Al-Henhen et al 2014; Boldbaatar et al 2014 ) with potential use in chemical ecology research, we here present results from 11 medicinal and culinary plants originating from Egypt. We isolated the essential oils and evaluated their bioactivity against the common tick I .…”

Section: Introductionmentioning

confidence: 99%

Essential oils of aromatic Egyptian plants repel nymphs of the tick Ixodes ricinus (Acari: Ixodidae)

et al. 2017

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Due to the role of Ixodes ricinus (L.) (Acari: Ixodidae) in the transmission of many serious pathogens, personal protection against bites of this tick is essential. In the present study the essential oils from 11 aromatic Egyptian plants were isolated and their repellent activity against I. ricinus nymphs was evaluated Three oils (i.e. Conyza dioscoridis L., Artemisia herba-alba Asso and Calendula officinalis L.) elicited high repellent activity in vitro of 94, 84.2 and 82%, respectively. The most active essential oil (C. dioscoridis) was applied in the field at a concentration of 6.5 µg/cm2 and elicited a significant repellent activity against I. ricinus nymphs by 61.1%. The most repellent plants C. dioscoridis, C. officinalis and A. herba-alba yielded essential oils by 0.17, 0.11 and 0.14%, respectively. These oils were further investigated using gas chromatography-mass spectrometry analysis. α-Cadinol (10.7%) and hexadecanoic acid (10.5%) were the major components of C. dioscoridis whereas in C. officinalis, α-cadinol (21.2%) and carvone (18.2%) were major components. Artemisia herba-alba contained piperitone (26.5%), ethyl cinnamate (9.5%), camphor (7.7%) and hexadecanoic acid (6.9%). Essential oils of these three plants have a potential to be used for personal protection against tick bites.

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“…Also, Yuan et al have shown that PA can be incorporated into plasma and erythrocytes and a part of PA can also be metabolized into cis 9, trans 11-18:2 in humans. The mechanism of in vivo transformation of PA into cis 9, trans 11-18:2 is not precisely known [16][17][18][19][20] .…”

Section: Review Articlementioning

confidence: 99%

Hatice Asli Bedel, Nur Tukel Turgut, Aysegul Ugur Kurtoglu, Coskun Usta

Bedel¹,

Turgut²,

Kurtoğlu³

et al. 2017

pharmaceutical-sciences

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Bedel, et al.: Pharmacology of Punicic AcidPlant extracts and nutraceuticals are the most ancient and widespread form of medication employed by the general population. The pomegranate is a prehistoric, mystical and a highly differentiated fruit. Moreover, pomegranate is found in some medicinal systems as a cure for a variety of illnesses. Pomegranate has been used for a long time for nutraceutical purposes. Current research indicates that the most medicinally useful pomegranate components include ellagitannins, anthocyanins, anthocyanidins, flavonoids, estrogenic flavonols and flavones. Also pomegranate seed oil contains 64-83% punicic acid. Therefore, this review focussed on the effects of punicic acid, particularly those that have been reported such as the anticarcinogenic, antioxidant, antiinflammatory and antidiabetic effects. As nutraceuticals appear to play a major role in the prophylaxis of various diseases, punicic acid could be an important and phytoconstituent among these agents. Nutraceuticals are generally regarded as safe to use with lower incidence of side effects. In spite of all these reports it is obvious that there is a clear need for more clinical studies.

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Antigenotoxic and antioxidant effects of the Mongolian medicinal plant Leptopyrum fumarioides (L): An in vitro study

Cited by 20 publications

References 29 publications

Evaluation of Potential DNA-Damaging Effects of Nitenpyram and Imidacloprid in Human U937-Cells Using a New Statistical Approach to Analyse Comet Data

Evaluation of Potential DNA-Damaging Effects of Nitenpyram and Imidacloprid in Human U937-Cells Using a New Statistical Approach to Analyse Comet Data

Essential oils of aromatic Egyptian plants repel nymphs of the tick Ixodes ricinus (Acari: Ixodidae)

Hatice Asli Bedel, Nur Tukel Turgut, Aysegul Ugur Kurtoglu, Coskun Usta

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