Antigenotoxic effect of acute, subacute and chronic treatments with Amazonian camu–camu (Myrciaria dubia) juice on mice blood cells

Silva, Francisco Carlos da; Arruda, Andrelisse; Ledel, Alexandre; Dauth, Cíntia; Romão, Nathalia Faria; Viana, Rafaele Nazário; Ferraz, Alexandre de Barros Falcão; Picada, Jaqueline Nascimento; Pereira, Patrı́cia

doi:10.1016/j.fct.2012.04.021

Cited by 35 publications

(25 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Mus musculus (CF-1™ strain) were used by Da Silva et al [27] to test the genotoxic and antigenotoxic potential of camu camu fruit juice after acute (single dose for one day), subacute (for 28 consecutive days), and chronic (for 56 consecutive days) oral administration. None of the fruit juice concentrations (25, 50, and 100%) tested exerted any genotoxic effect on blood cells in male and female mice.…”

Section: Dna Damage and Cancer Protection Effectsmentioning

confidence: 99%

“…These associated beneficial effects of phytochemicals were corroborated by numerous in vitro and in vivo studies with several animal models (i.e., flies, mice, rats, etc.) and human volunteers [16,17,22,[24][25][26][27][28]. In the healthpromoting phytochemicals section of the book chapter, we will illustrate the chemical structures of several of these phytochemicals that were isolated from various tissues of camu camu by bioassay-guided approaches.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Myrciaria dubia “Camu Camu” Fruit: Health-Promoting Phytochemicals and Functional Genomic Characteristics

Gómez¹,

Maddox²,

Cobos³

et al. 2018

Breeding and Health Benefits of Fruit and Nut Crops

View full text Add to dashboard Cite

Camu camu is a typical Amazon native fruit shrub that possesses a diploid genome, moderate genetic diversity, and population structure. The fruits accumulate several essential nutrients and synthesize L-ascorbic acid (vitamin C) in great quantities and an array of diverse secondary metabolites with corroborated in vitro and in vivo health-promoting activities. These beneficial effects include antioxidative and antiinflammatory activities, antiobesity, hypolipidemic, antihypertensive and antidiabetic effects, DNA damage and cancer protection effects, and other bioactivities. Many health-promoting phytochemicals are biosynthesized in several metabolic pathways of camu camu. Their reconstruction from the fruit transcriptome database was accomplished by our research group. These include basic metabolic pathways such as glycolysis and pentose phosphate pathway, vitamin C biosynthesis pathways, and pathways involved in secondary metabolites production. Due to their agronomic potential and fruits growing demand, recently, based on an ideotype, programs were initiated for their domestication and genetic improvement, but so far with very negligible achievements. Consequently, we propose new strategies to accelerate the processes of domestication and genetic improvement based on state of the art technologies for multiomic data analysis and innovative molecular tools.

show abstract

Section: Dna Damage and Cancer Protection Effectsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Myrciaria dubia “Camu Camu” Fruit: Health-Promoting Phytochemicals and Functional Genomic Characteristics

Gómez¹,

Maddox²,

Cobos³

et al. 2018

Breeding and Health Benefits of Fruit and Nut Crops

View full text Add to dashboard Cite

show abstract

“…Other qualities have been reported for camu camu in animals: Gonzáles et al [] demonstrated the potential effects of M. dubia in improving spermatogenesis (increased stages of mitosis, meiosis, and spermiation) as well as increasing daily production and sperm count per epididymis. da Silva et al [] reported the antioxidant, anti‐genotoxic potential of camu camu juice in mouse blood cells after chronic, acute, and sub‐acute treatments, by the assay 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) (in vitro antioxidant activity) and comet assay (genotoxic and anti‐genotoxic activity).…”

Section: Introductionmentioning

confidence: 99%

Myrciaria dubia “camu camu” flour as a magnetoprotector in male mouse infertility

Torres

Guevara

Cruz

et al. 2019

Bioelectromagnetics

View full text Add to dashboard Cite

Possible adverse effects of extremely low frequency magnetic fields (ELF‐MF) are linked to a decrease of antioxidant defenses and the production of free radicals. The increase of free radicals affects the mitochondrial functionality inducing apoptosis, which affects the phosphorylation and generation of key ATP in fertilization. Myrciaria dubia, known as “camu camu,” is a fruit with high levels of ascorbic acid, which exerts an important antioxidant function in the prevention of premature cell damage. In this study, the effect of Myrciaria dubia flour on oxidative damage produced by ELF‐MF (610 μT) was evaluated by detecting the activity of endogenous mitochondrial oxidoreductase enzymes in a complete sperm cycle of mice. We found that the MF caused a significant (P < 0.05) decrease in sperm quality, whereas the groups supplied with Myrciaria dubia flour (50 and 75 mg/kg of body mass) in ELF‐MF exposure showed a significant recovery (P < 0.05) in parameters of viability, percentage of plasma membrane integrity and mitochondrial activity, and index of epidymal sperm. This suggests that Myrciaria dubia flour would have an antioxidant activity that counteracts the damaging effects of ELF‐MF in spermatogenesis and could be used as a natural ELF‐MF protector. Bioelectromagnetics. 40:91–103, 2019. © 2019 Bioelectromagnetics Society

show abstract

“…Asimismo, sus frutos contienen antocianinas como cianidina-3-glucósido (Villanueva et al, 2010), carotenoides como luteína (Zanatta y Mercadante, 2007; Azevedo-Meleiro y Rodríguez-Amaya, 2009) y otras sustancias con potencial uso farmacológico (Akachi et al, 2010;Yazawa et al, 2011;Da Silva et al, 2012;Nascimento et al, 2013). Por estas cualidades los frutos de esta especie tienen gran demanda en mercados locales, nacionales e internacionales.…”

Section: Introductionunclassified

Un método eficiente para la inducción de callos in vitro en Myrciaria dubia (Kunth) Mc Vaugh "Camu Camu"

Córdova

Cobos

Imán³

et al. 2014

Sci. agropecu

View full text Add to dashboard Cite

ResumenDebido a la alta variabilidad en la producción de vitamina C en Myrciaria dubia "camu camu", es necesario establecer procedimientos biotecnológicos para la propagación clonal masiva de genotipos promisorios de esta especie. El objetivo fue establecer un método eficiente para inducir la formación de callos in vitro a partir de explantes de M. dubia. Los explantes de hojas y nudos se obtuvieron de ramas cultivadas en el laboratorio y la pulpa a partir de frutos colectados en el campo. Estos fueron desinfectados y sembrados en medio MurashigeSkoog (1962) suplementado con ácido 2,4-diclorofenoxiacético (2,4-D), bencilaminopurina (BAP) y kinetina (Kin). Los cultivos fueron mantenidos a 25±2°C, en oscuridad por 2 semanas y posteriormente con un fotoperiodo de 16 horas luz y 8 horas de oscuridad por 6 semanas. El tratamiento con 2 mg/L de 2,4-D y 0,1 mg/L de BAP estimuló mayor callogénesis en los tres tipos de explantes. Los callos se generaron a partir de la primera semana (nudos), cuarta semana (hojas) y sexta semana (pulpa) y estos fueron friables (hojas y nudos) y no friables (pulpa). En conclusión, el método descrito es eficiente para inducir callos in vitro en hojas, nudos y pulpa de M. dubia, siendo los explantes de hojas y nudos los más idóneos para la obtención de callos. Palabras clave: callogénesis, propagación clonal, camu camu, in vitro, Myrciaria dubia. AbstractDue to the high variability in vitamin C production in Myrciaria dubia "camu camu", biotechnological procedures are necessary for mass clonal propagation of promising genotypes of this species. The aim was to establish an efficient method for in vitro callus induction from explants of M. dubia. Leaf and knot sex plants were obtained from branches grown in the laboratory and from fruit pulp collected in the field. These were desinfected and sown on Murashige-Skoog (1962) medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D), benzylaminopurine (BAP) and kinetin(Kin). The cultures were maintained at 25±2°C in darkness for 2 weeks and subsequently with a photoperiod of 16 hours in light and 8 hours in dark for 6 weeks. Treatment with 2 mg/L 2,4-D and 0.1 mg/L BAP allowed major callus formation in the three types of explants. Calluswere generated from the first week (knots), fourth week (leaves) and sixth week (pulp) and these were friable (leaves and nodes) and non-friable (pulp). In conclusion, the described method is efficient for in vitro callus induction in leaves, knots and pulp of M. dubia, been leaves and knots explants more suitable for callus obtention.

show abstract

Antigenotoxic effect of acute, subacute and chronic treatments with Amazonian camu–camu (Myrciaria dubia) juice on mice blood cells

Cited by 35 publications

References 25 publications

Myrciaria dubia “Camu Camu” Fruit: Health-Promoting Phytochemicals and Functional Genomic Characteristics

Myrciaria dubia “Camu Camu” Fruit: Health-Promoting Phytochemicals and Functional Genomic Characteristics

Myrciaria dubia “camu camu” flour as a magnetoprotector in male mouse infertility

Un método eficiente para la inducción de callos in vitro en Myrciaria dubia (Kunth) Mc Vaugh "Camu Camu"

Contact Info

Product

Resources

About