Antigenotoxic potential of gel extract of Aloe vera against Sodium azide genotoxicity in Allium cepa cells

Abstract

Nowadays, the increasing rate of human exposure to various kinds of environmental mutagens has necessitated the search for natural antimutagens /antigenotoxic agents in natural products. In this study, Aloe vera gel extract was tested for its possible antigenotoxicity following the Allium cepa assay. Ten onions (Allium cepa) per dose were grown for 48 and 72 hours on gel extract of A. vera at 6.25%, 12.5%, 25.0%, 50.0% and 100.0% in combination with sodium azide (0.05mg/ml) solution for microscopic and macroscopic evaluations, respectively. Distilled water and sodium azide were the negative and positive controls, respectively. The cell division in the root tips, and root growth in the exposed A. cepa were inhibited in a dose dependent manner by the mixture of A. vera and sodium azide. However, the mixture of absolute (100.0%) dose and sodium azide completely arrested cell division and induced a lower root length than that recorded for sodium azide alone. The genotoxicity of sodium azide was inversely reduced by the doses of A. vera except at 100.0%. These results show that gel extract of A. vera possesses strong antigenotoxic /antimutagenic potency at lower dose range of 6.25% to 25.0% in A. cepa cells, however, its higher doses above 50.0% to 100.0% could be severely toxic when being considered for suppression of environmental mutagens’ mutagenicity or genotoxicity. This suggests that gel extract of A. vera contains phytochemical(s) that can be useful in the development of anticancer drug.

Drogas usadas como controles positivos nos ensaios de toxicidade com Allium cepa L.: uma revisão da literatura

“…O composto é reconhecido por sua capacidade de induzir aberrações cromossômicas, incluindo aderências anormais, formação de pontes, alterações na divisão mitótica, presença de cromossomos vagantes, anomalias nas estruturas nucleares celulares, desordens no funcionamento do fusomitótico e até mesmo ocorrência de quebras cromossômicas. Além de que, também possui capacidade de impedir a condensação da cromatina e diminuir o índice mitótico (Prieto et al, 2006;Abdulrahaman et al, 2018;Akinboro;Jimoh, 2021). Contudo, é importante ressaltar que um aspecto desfavorável da AS é sua resposta mutagênica variável, influenciada pelo pH da solução de tratamento.…”

Protective Effects of Aloe Vera Extract Administiration against Trifluralin in Drosophila melanogaster with Various Parameters

“…is a clear and gel-like substance obtained from A. vera leaf [13]. A. vera plant is known to contain active components such as vitamins, enzymes, minerals, sugars, lignin, saponins, salicylic acids and amino acids [14]. It is reported that A. vera, which has anticarcinogenic, antibacterial, antiviral, antiinflammatory and antioxidant activities, reduces the genotoxic effect of D. melanogaster reduced genotoxicity after D. melanogaster had mutated with ethyl methanesulfonate (EMS) [15].…”

Evaluation of an antimalarial herbal mixture and each extract for DNA and chromosomal mutations in Swiss albino mice and Allium cepa cells

“…The powder of each plant and that of the recipe in separate glass jars was added with five hundred milliliters of distilled water and then placed inside a water bath set at 64°C for 2 hours 15 minutes. The single extract and recipe were allowed to cool down before sieving each through a Whatman filter paper (No 1) and stored in a refrigerator at 4ºC for further use (Akinboro et al, 2017;Akinboro and Jimoh, 2021a).…”

“…They were sun dried for two weeks to reduce moisture content so as to facilitate root growth. The onions were descaled carefully using a razor blade, leaving intact the primordial root ring (Akinboro et al, 2020b;Akinboro et al, 2021aAkinboro et al, , 2021b. The onions were rinsed with distilled water and wiped with tissue paper.…”

“…They were then hydrolyzed inside 1N hydrochloric acid in a petri dish, then placed in an oven set at 6 ˚ for minutes. he hydrolyzed roots were treated for slides preparation as previously described (Akinboro et al, 2020b, Akinboro andJimoh, 2021a;Akinboro et al,2021b). The prepared slides were observed under a binocular light microscope (PEC, Medical, USA) using immersion oil objective lens.…”