Antigens of gastric and intestinal mucous cells in human colonic tumours

Bara, Jacques; Loisillier, F; Burtin, P

doi:10.1038/bjc.1980.32

Cited by 99 publications

(58 citation statements)

References 11 publications

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“…Despite the differences between patterns of mucin heterogeneity observed with anti-HCM MAbs and other methods within the colonic mucosa, the increasing cross-reactivity ofanti-HCM MAbs at progressively more distal sites of the gastrointestinal tract is comparable to the results of earlier studies (29,34). The present studies confirm the impression that some structural features are common to all gastrointestinal tract mucin, while other components of mucin may represent organ specific determinants.…”

Section: Discussionsupporting

confidence: 80%

Human colonic goblet cells. Demonstration of distinct subpopulations defined by mucin-specific monoclonal antibodies.

1986

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We studied glycoprotein content of human colonic goblet cells, using a library of monoclonal antibodies (MAbs) Anti-HCM MAbs stained goblet cells from other sites within the gastrointestinal tract to a varying extent. Anti-HCM MAbs also showed extensive cross-reactivity with rodent, rabbit, and monkey colonic mucosa. However, several anti-HCM MAbs stained only human colonic mucosa. These data show that human colonic mucosa contains discrete subpopulations of goblet cells that produce distinctive combinations of specific mucin glycoprotein species.

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Section: Discussionsupporting

confidence: 80%

Human colonic goblet cells. Demonstration of distinct subpopulations defined by mucin-specific monoclonal antibodies.

1986

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“…Indeed, several earlier studies have utilized probes directed to circumscribed nonreducing terminal structures to explore colonic mucin heterogeneity. These probes have included lectins that recognize terminal monosaccharides or disaccharides (18,22,33,34), histochemical stains that bind peripheral charged residues (1 1, 30, 35, 36) or conventional antisera directed at limited peripheral carbohydrate determinants (17,37,38). The present results suggest that relatively nonspecific tools may both underestimate heterogeneity and overestimate structural homologies.…”

Section: Discussionmentioning

confidence: 79%

Development of anti-human colonic mucin monoclonal antibodies. Characterization of multiple colonic mucin species.

1986

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Structural relationships between colonic mucin species were assessed using a library of monoclonal antibodies (MAbs) directed against purified human colonic mucin (HCM). After immunization of mice with purified mucin from normal human colonic mucosa, 14% of 1,920 fusion products screened were positive for anti-HCM activity in a solid-phase assay. Patterns of selective binding by hybridomas to six discrete HCM species (I-VI) separated by DEAE-cellulose chromatography suggested the presence of both shared and species-specific antigenic determinants among HCM species I-VI. 23 anti-HCMs MAbs (7 IgM,7 IgGj, and 9 IgG2), demonstrating a range of anti-HCM species specificities, were produced and used to study structural relationships between mucin species. Binding of various mucin species by individual anti-HCM MAbs was shown by competitive solid-phase radioimmunoassay to reflect the presence of identical epitopes on the different species. Adsorption of HCM species on a variety of affinity resins prepared with anti-HCM MAbs demonstrated that binding to multiple mucin species by a single MAb was related to intrinsic structural determinants. Four anti-HCM MAbs recognized protease-sensitive antigenic structures, which suggests that they may be directed to core HCM proteins. 12 of the anti-HCM MAbs were shown by solid-phase assay to recognize either complete (n = 5) or partial (n = 7) isolated colonic mucin oligosaccharide side chains of defined structure. Collectively, these data show the presence of both shared and unique antigenic structural determinants among colonic mucin species. Chromatographic heterogeneity of mucin glycoproteins seems to be related to the existence of biologically significant subclasses in the normal human colon.

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“…[15][16][17][18] HGM is a specific marker for core protein of gastric foveolar cells. 19,20) The correlations between positivity to these antibodies and clinicopathologic features suggested that cell differentiation is inversely associated with tumor growth and aggressiveness.…”

Section: Discussionmentioning

confidence: 99%

“…We selected three immunohistochemical markers for cell differentiation: MUC2 for intestinal goblet cells, [12][13][14] CD10 for small intestinal brush border [15][16][17][18] and human gastric mucin (HGM) for gastric differentiation. 19,20) A representative section from each tumor was processed for immunohistochemical staining with monoclonal antibodies against MUC2 (Novocastra, Newcastle-upon-Tyne, UK, diluted 1:200), CD10 (Novocastra, diluted 1:200) and HGM (45M1, Novocastra, diluted 1:50). Four-micron-thick sections were cut, deparaffinized in xylene and dehydrated in an ethanol series.…”

Section: Methodsmentioning

confidence: 99%

Phenotypic Expression of Colorectal Adenocarcinomas with Reference to Tumor Development and Biological Behavior

Yao

Tsutsumi

Akaiwa

et al. 2001

Japanese Journal of Cancer Research

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The purpose of this study is to clarify the correlation between cell differentiation and tumor development, including tumor aggressiveness and biological behavior. Eighty-three cases of advanced colorectal adenocarcinoma were randomly selected. Using immunohistochemical staining with antibodies to CD10, MUC2 and human gastric mucin (HGM), the colorectal adenocarcinomas could be classified into five types (18 small intestinal, 27 large intestinal, 2 gastric, 9 mixed and 27 unclassified). Each type had characteristic features. The small-intestinal type showed a relatively lower incidence of lymphatic permeation and higher venous invasion. The large-intestinal type showed a low incidence of venous invasion and lymph node metastasis. The mixed type revealed female and right-side-dominant distribution, large tumor size, high incidence of mucinous carcinoma, and low incidence of venous invasion. Gastric type was seen in only two cases (2%), which exhibited high histologic grade, lymphatic permeation and lymph node metastasis with no venous invasion. Such phenotypic classifications are considered to be useful not only for evaluation of the biological behavior of the carcinoma, but also for analysis of tumorigenesis. Key words: Colorectal carcinoma -Pheotype -MUC2 -CD10 -Human gastric mucinOn the basis of the Lauren classification, 1) gastric carcinomas were classified into two types, intestinal type and diffuse type. Following recent advances in mucin histochemistry and immunohistochemistry, it has been clarified that differentiated adenocarcinomas can be classified into two subtypes, gastric and intestinal phenotypes. 2, 3)Some authors reported that the phenotypic expression was related to the tumor growth pattern and aggressiveness. 4,5) We have already suggested that gastric carcinomas could be classified into four types (small intestinal, incomplete intestinal, gastric, and undifferentiated) by analogy with intestinal metaplasia. 6,7)

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Antigens of gastric and intestinal mucous cells in human colonic tumours

Cited by 99 publications

References 11 publications

Human colonic goblet cells. Demonstration of distinct subpopulations defined by mucin-specific monoclonal antibodies.

Human colonic goblet cells. Demonstration of distinct subpopulations defined by mucin-specific monoclonal antibodies.

Development of anti-human colonic mucin monoclonal antibodies. Characterization of multiple colonic mucin species.

Phenotypic Expression of Colorectal Adenocarcinomas with Reference to Tumor Development and Biological Behavior

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