Antigens of Streptococcus mutans: characterization of a polysaccharide antigen from walls of strain GS-5

Wetherell, Jr Jr; Bleiweis, A S

doi:10.1128/iai.12.6.1341-1348.1975

Cited by 40 publications

(32 citation statements)

References 18 publications

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“…Other non-peptidoglycan components, glucose, rhamnose, and/or galactose, were variably detected in S. mutans cell walls (Table 3). These results are consistent with previous observations (1,16), and support the findings that the serotype a, d, and g antigens are polymers of galactose and glucose (19,26,27,34), while the serotype c, e, and f antigens are polymers of glucose and rhamnose (10,14,48). Serotype b antigen has been shown to be a polymer of rhamnose and galactose (33).…”

Section: Discussionsupporting

confidence: 92%

Chemical Composition of Streptococcus mutans Cell Walls and Their Susceptibility to Flavobacterium L‐11 Enzyme

Inoue

Hamada

Ooshima

et al. 1979

Microbiology and Immunology

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The susceptibility to a cell wall lytic L-11 enzyme from Flavobacterium sp. and the quantitative and/or qualitative composition of the cell walls of some strains of cariogenic Streptococcus mutans and a non-cariogenic strain of Streptococcus mitis were determined. The purified cell walls of S. mutans strains HS-1 (serotype a),(f), OMZ65 (g), and AHT (g), and S. mitis CHT contained glutamic acid, alanine, and lysine as well as muramic acid and glucosamine as a peptidoglycan component. Besides these amino acids, significant amounts of threonine were detected in strains HS-1, OMZ65, and AHT cell walls, and considerable amounts of aspartic acid and/ or threonine as well as several other amino acids in OMZ 176, OMZ65, and CHT cell walls. Rhamnose was a common special component of the cell walls of S. mutans strains BHT, NCTC10449, MT703, B2 (e), MT557, and AHT, and S. mitis CHT. An additional sugar component, glucose, was detected in the cell walls of all of these strains except BHT, and galactose was found in BHT, AHT, and CHT cell walls. Galactosamine was present in S. mitis CHT cell walls. Varying amounts of phosphorus were detected in the cell walls of all the strains examined. The cell walls of all these streptococcal strains except MT703, 6715, and AHT were susceptible to the lytic action of the L-11 enzyme to various extents. No consistent relationship was observed between the amino acid and sugar composition of these cell walls and their susceptibility to the L-11 enzyme. The chemical composition of these cell walls is discussed in terms of the serological classification of S. mutans.

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Section: Discussionsupporting

confidence: 92%

Chemical Composition of Streptococcus mutans Cell Walls and Their Susceptibility to Flavobacterium L‐11 Enzyme

Inoue

Hamada

Ooshima

et al. 1979

Microbiology and Immunology

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“…Antibodies against GTase(s) inhibit enzyme activity (1,6,7,9,10,21,23,35) and result in the reduction of experimental caries of S. mutans in rats (37). 5. mutans has been classified into seven serotypes (3,14,15,31) of which immunological specificity is based on the cell wall polysaccharide (12,17,22,23,26,27,40). Questions have arisen concerning the immunological properties of GTase in view of the importance of this enzyme in dental caries and its possible use as an immunizing agent.…”

mentioning

confidence: 99%

Serotype‐Dependent Inhibition of Glucan Synthesis and Cell Adherence of Streptococcus mutans by Antibody against Glucosyltransferase of Serotype e S. mutans

Hamada

Tai

Slade

1979

Microbiology and Immunology

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“…Strains of Streptococcus mutans, an agent that develops dental caries in animals and probably humans, have been subdivided into seven immunological types (2,30). The antigens of types a (27), b (26), c (16,42), d (13,18) and f (8a) are located in the cell wall and are polysaccharides composed of rhamnose and glucose (type c and f), rhamnose and galactose (type b), and glucose and galactose (types a and d). S. mutans strains do not show any immunological cross-reaction with the streptococcal groups except group E (3,30).…”

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confidence: 99%

Purification and immunochemical characterization of type e polysaccharide antigen of Streptococcus mutans

Hamada¹,

Slade²

1976

Infect Immun

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The type-specific antigen of Streptococcus mutans strain MT703, serotype e, has been chromatographically purified and characterized. Two chromatographic fractions were obtained from saline extracts which reacted with both anti-MT703 whole-cell serum and Lancefield group E serum. The major fraction (eI) was identified as a polysaccharide composed of 37% glucose, 56% rhamnose, 5% protein, and 0.3% phosphorus, whereas the minor fraction (eII) contained 66% protein in addition to 10% glucose and 17% rhamnose. The immunological specificity of these antigens was found to be the same by immunodiffusion in agar gel. Another fraction with a negative charge (eIII) reacted with polyglycerophosphate antisera from Streptococcus mutans and Streptococcus pyogenes. For comparison, the MT703 antigen in a hot trichloroacetic acid extract (eA) and the group E antigen from a saline extract of cells of strain K129 (EI) were similarly purified by anionic ion-exchange chromatography. Although the ratio of glucose and rhamnose in eA was 1:0.9 and in eI and eII approximately 1:1.5, reactions of identity were obtained in gel diffusion against specific anti-e serum. This difference in ratio is probably a result of the extraction procedures. Both the type e and group E antisera were reactive with both eI and EI antigens. The adsorption of group E antiserum with MT703 cells removed all E antibody, whereas type e-specific antibody remained after adsorption with K129 cells. These results suggest that eI antigen possesses both e and E specificities, whereas EI possesses E only. These findings were supported by the quantitative precipitin test and immunodiffusion and/or immunoelectrophoretic patterns in agar gel. Methyl-,-i->glucopyranoside markedly inhibited the precipitin reaction in both type e and group E sera. However, a significantly stronger inhibition by cellobiose of type e serum than of group E serum indicates that a ,-linked glucose-glucose dimer is the predominant antigenic determinant of the e specificity. The presence of both e and E specificities on a single polysaccharide molecule was demonstrated by the use of purified e antigen released from a specific e-anti-e complex. This antigen reacted with a group E-specific serum as well as a type e-specific serum. An examination of five S. mutans type e strains showed the presence of group E specificity also, whereas the I, II, and IV serotypes of group E streptococci only possessed the group E specificity. Strains of Streptococcus mutans, an agent that develops dental caries in animals and probably humans, have been subdivided into seven immunological types (2, 30). The antigens of types a (27), b (26), c (16, 42), d (13, 18) and f (8a) are located in the cell wall and are polysaccharides composed of rhamnose and glucose (type c and f), rhamnose and galactose (type b), and glucose and galactose (types a and d). S. mutans strains do not show any immunological cross-reaction with the streptococcal groups except group E (3, 30).Bratthall (3) considered that type e S. mutans possessed the ...

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Antigens of Streptococcus mutans: characterization of a polysaccharide antigen from walls of strain GS-5

Cited by 40 publications

References 18 publications

Chemical Composition of Streptococcus mutans Cell Walls and Their Susceptibility to Flavobacterium L‐11 Enzyme

Chemical Composition of Streptococcus mutans Cell Walls and Their Susceptibility to Flavobacterium L‐11 Enzyme

Serotype‐Dependent Inhibition of Glucan Synthesis and Cell Adherence of Streptococcus mutans by Antibody against Glucosyltransferase of Serotype e S. mutans

Purification and immunochemical characterization of type e polysaccharide antigen of Streptococcus mutans

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