Antiglycation study of HMG-R inhibitors and tocotrienol against glycated BSA and LDL: A comparative study

Nabi, Rabia; Alvi, Sahir Sultan; Khan, Rizwan Hasan; Ahmad, Shafeeque; Ahmad, Saheem; Khan, M. Salman

doi:10.1016/j.ijbiomac.2018.05.115

Cited by 39 publications

(62 citation statements)

References 44 publications

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“…In addition, plasma from diabetic subjects also contain increased levels of CD, LOOH, TBARS, and/or MDA 37 . Persistent oxidative stress and elevated glucose levels in various tissues e.g., liver and kidney, also lead to the peroxidative and glycoxidative modification of lipids which ultimately leads to the formation of TBARS and various advanced glycation end products (AGEs) 1,38 . We also evident a significant increase in the plasma, as well as hepatic and renal lipoperoxidative events, in DC rats that could be attributed to the enhanced rate of free radical generation under hyperglycemia and subsequent generation of CD, LOOH and MDA 37 .…”

Section: Discussionmentioning

confidence: 99%

Therapeutic efficacy of Boerhaavia diffusa (Linn.) root methanolic extract in attenuating streptozotocin-induced diabetes, diabetes-linked hyperlipidemia and oxidative-stress in rats

et al. 2019

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Introduction:We have previously demonstrated that sequentially extracted methanolic fractions of Boerhaavia diffusa (Linn.) showed marked antioxidant, antidiabetic and oxidative-DNA damage protective properties in vitro. The present study was undertaken to evaluate the beneficial effects of B.diffusa (Linn.) methanolic root extract and its partially purified bioactive fraction on streptozotocin (STZ)-induced hyperglycemia and hyperlipidemia in rats. Methods: The diabetic rats were treated for fourteen weeks either with methanolic extract of B. diffusa root (D-MT1, D-MT2, and D-MT3 : doses of 50, 150, and 300 mg/rat/day, respectively), partially isolated bioactive fraction (D-BT: 0.5 mg/rat/day), or glibenclamide (D-GT: 0.5 mg/rat/day). Results: The level of fasting blood glucose (FBG) and glycated hemoglobin (HbA1c) were significantly alleviated in D-MT-and D-BTtreated groups after fourteen weeks of administration. Moreover, plasma lipid profile, free fatty acids (FFAs), phospholipids (PLs), HMG-CoA reductase (HMG-R) activity, conjugated diene (CD), lipid hydroperoxide (LOOH), and malondialdehyde (MDA) were also markedly ameliorate d in all treatment groups. In addition, the activity of antioxidant enzymes, e.g., superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (Gred), and glutathione-S-transferase (GST), were also significantly restored by D-MT -and D-BT -treated groups. Furthermore, histologically, all the unseemly features of nephropathy were extensively regressed and normalized by the administration of B. diffusa and its bioactive fraction. Conclusions: Our results demonstrate a strong antidiabetic and hypolipidemic impact of B. diffusa extract an ideal alternative therapeutic agent in the prevention and treatment of diabetes as well as diabetes-linked hyperlipidemia.Cite this article : Akhter F, Alvi S S, Ahmad P, Iqbal D, Alshehri B M, Khan M S. Therapeutic efficacy of Boerhaavia diffusa (Linn.) root methanolic extract in attenuating streptozotocin-induced diabetes, diabetes-linked hyperlipidemia and oxidative-stress in rats. Biomed. Res. Ther.; 6(7):3293-3306.

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Section: Discussionmentioning

confidence: 99%

Therapeutic efficacy of Boerhaavia diffusa (Linn.) root methanolic extract in attenuating streptozotocin-induced diabetes, diabetes-linked hyperlipidemia and oxidative-stress in rats

et al. 2019

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“…Protein-bound carbonyls were determined spectrophotometrically with the use of the carbonyl-specific reagent DNPH [41,42]. Amounts of 250 μL of native LDL or HDL and each oxidized lipoprotein were mixed with 350 μL of 7 mM DNPH in 2 N HCl.…”

Section: Methodsmentioning

confidence: 99%

Anti-Atherosclerotic Effects of Fruits of Vitex rotundifolia and Their Isolated Compounds via Inhibition of Human LDL and HDL Oxidation

Kim

Shim

2019

Biomolecules

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Low-density lipoprotein (LDL) and high-density lipoprotein (HDL) oxidation are well known to increase the risk for atherosclerosis. In our ongoing research on natural products with inhibitory activities against oxidation of lipoproteins, fruits of Vitex rotundifolia were found to be highly active. There is no report on the effects on LDL and HDL oxidation. Herein, we investigated the inhibitory effects of V. rotundifolia fruit extract and its six compounds, which are: (1) artemetin, (2) casticin, (3) hesperidin, (4) luteolin, (5) vitexin, and (6) vanillic acid, against LDL and HDL oxidation. The LDL and HDL oxidations were determined by measuring production of conjugated dienes and thiobarbituric acid reactive substances, amount of hyperchromicity and carbonyl content, change in electrical charge, and apoA-I aggregation. In addition, the contents of the compounds in the extracts were analyzed using HPLC-DAD. Consequently, extracts of Vitex rotundifolia fruits and compounds 2 and 4 suppressed oxidation of LDL and HDL, showing inhibition of lipid peroxidation, decrease of negative charges in lipoproteins, reduction of hyperchromicity, decrease in carbonyl contents, and prevention of apoA-I aggregation. In particular, compounds 2 and 4 exhibited more potent inhibitory effect on oxidation of LDL and HDL than the extracts, suggesting their protective role against atherosclerosis via inhibition of LDL and HDL oxidation. The contents of artemetin, casticin, and vanillic acid in the extracts were 1.838 ± 0.007, 8.629 ± 0.078, and 1.717 ± 0.006 mg/g, respectively.

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“…Protein-bound carbonyls were measured using the carbonyl-specific reagent 2,4-dinitrophenylhydrazine (DNPH) [36,37]. First, 250 µL of native or oxidized samples were mixed with 350 µL of DNPH solution (7 mM DNPH in 2 N HCl).…”

Section: Measurement Of Protein-bound Carbonyl Groupsmentioning

confidence: 99%

Anti-Atherosclerotic Activity of (3R)-5-Hydroxymellein from an Endophytic Fungus Neofusicoccum parvum JS-0968 Derived from Vitex rotundifolia through the Inhibition of Lipoproteins Oxidation and Foam Cell Formation

Kim

Shim

2020

Biomolecules

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An endophytic fungus, Neofusicoccum parvum JS-0968, was isolated from a plant, Vitex rotundifolia. The chemical investigation of its cultures led to the isolation of a secondary metabolite, (3R)-5-hydroxymellein. It has been reported to have antifungal, antibacterial, and antioxidant activity, but there have been no previous reports on the effects of (3R)-5-hydroxymellein on atherosclerosis. The oxidation of lipoproteins and foam cell formation have been known to be significant in the development of atherosclerosis. Therefore, we investigated the inhibitory effects of (3R)-5-hydroxymellein on atherosclerosis through low-density lipoprotein (LDL) and high-density lipoprotein (HDL) oxidation and macrophage foam cell formation. LDL and HDL oxidation were determined by measuring the production of conjugated dienes and malondialdehyde, the amount of hyperchromicity and carbonyl content, conformational changes, and anti-LDL oxidation. In addition, the inhibition of foam cell formation was measured by Oil red O staining. As a result, (3R)-5-hydroxymellein suppressed the oxidation of LDL and HDL through the inhibition of lipid peroxidation, the decrease of negative charges, the reduction of hyperchromicity and carbonyl contents, and the prevention of apolipoprotein A-I (ApoA-I) aggregation and apoB-100 fragmentation. Furthermore, (3R)-5-hydroxymellein significantly reduced foam cell formation induced by oxidized LDL (oxLDL). Taken together, our data show that (3R)-5-hydroxymellein could be a potential preventive agent for atherosclerosis via obvious anti-LDL and HDL oxidation and the inhibition of foam cell formation.

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Antiglycation study of HMG-R inhibitors and tocotrienol against glycated BSA and LDL: A comparative study

Cited by 39 publications

References 44 publications

Therapeutic efficacy of Boerhaavia diffusa (Linn.) root methanolic extract in attenuating streptozotocin-induced diabetes, diabetes-linked hyperlipidemia and oxidative-stress in rats

Therapeutic efficacy of Boerhaavia diffusa (Linn.) root methanolic extract in attenuating streptozotocin-induced diabetes, diabetes-linked hyperlipidemia and oxidative-stress in rats

Anti-Atherosclerotic Effects of Fruits of Vitex rotundifolia and Their Isolated Compounds via Inhibition of Human LDL and HDL Oxidation

Anti-Atherosclerotic Activity of (3R)-5-Hydroxymellein from an Endophytic Fungus Neofusicoccum parvum JS-0968 Derived from Vitex rotundifolia through the Inhibition of Lipoproteins Oxidation and Foam Cell Formation

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