Antihelminthic Activity of Lophira Lanceolata on Heligmosomoides polygyrus Using an Automated High-Throughput Method

Abstract

Background. There are about 13 parasitic infections that are responsible for significant morbidity and mortality but have not received the attention they deserve; thus, they are now known as “neglected tropical diseases” (NTDs). This study was aimed at evaluating the antihelminthic activities of Lophira lanceolata using an automated high-throughput method. Methods. The antihelminthic activity effect of the extracts against H. polygyrus was determined using an automated high-throughput method. For the egg-hatching test, 100 μL of embryonated egg suspension (60 eggs) was added to 100 μL of various concentrations of extracts, levamisole, and 1.5% DMSO in a 96-well round-bottom microtitre plate. After mixing, the 96-well microplate was placed in WMicroTracker and incubated for 24 h at 25°C; the movements were recorded every 30 minutes. The same procedure was used for the larval motility assays, where 100 μL of L1 or L2 larvae (50 larvae) were put in contact with 100 μL of various concentrations of extracts. Results. The ovicidal activity (hatching) had an IC50 of 1.4 mg/mL for the ethanol extract. The aqueous and ethanol extracts of L. lanceolata showed larvicidal activity on the L1 larvae with IC50 of 1.85 mg/mL and 2.4 mg/mL, respectively, as well as on the L2 larvae with IC50 values of 1.08 mg/mL and 1.02 mg/mL for the aqueous and ethanol extracts, respectively. These results showed that the aqueous extract exhibited a stronger inhibitory power on the hatching rate of parasites than ethanol extracts, while the contrary effect was observed for the larval motility assays. Conclusion. This study provides scientific data on the use of L. lanceolata by the local population for the treatment of helminthiases. However, in vivo and toxicity tests are necessary to assess its activity and safety.

Nematocidal Activity of Ethanol and Aqueous Extracts of Persea americana Seeds against Heligmosomoides polygyrus using the Worm Microtracker Method

“…The larvicidal activity was determined using the worm microtracker method described by Cédric et al [ 13 ]. The L1 and L2 larvae were put in contact with extracts with the same concentrations as the ovicidal activity (5–0.3125 mg/mL) and incubated for 24 hours in a final volume of 200 μ L. The percentage of inhibition of larvae motility was determined [ 24 ].…”

“…Eggs. The collection of eggs was done using the flotation technique described by Cédric et al [13]. After the collection of fresh eggs, it was incubated at 25 °C for 24 hours for the obtention of embryonated eggs and used for the evaluation of hatching.…”

“…Medicinal plants are often used by traditional healers in the western region of Cameroon to overcome this problem of resistance, high cost, and availability [ 13 ]. The misuse of synthetic drugs is now a serious challenge in the domain of drug discovery.…”

Antimalarial Efficacy and Antioxidant Activity of Lophira lanceolata Stem Bark Ethanol Extract Using Plasmodium berghei Induced-Malaria in Swiss Albino’s Mice

“…Barks. The ethanol extract was obtained using the method described by Cedric et al [10]. Briefly, 100 g of L. lanceolata powder was introduced into 1 l of 95% ethanol and remained in the solvent for 72 hours with occasional stirring.…”

“…The ethanol extract showed interesting plasmodial activity, revealing an IC 50 of 31.865 and 24.515 μ g/ml for the sensitive and resistant strains, respectively, and a resistance index of 0.01 (IR = 0.01). et al [ 10 ] recently demonstrated the antihelminthic activity of L. lanceolata . Similarly, according to Sinan et al [ 11 ], the chemical profiling of L. lanceolata confirmed the presence of lanceolatins and lophirones.…”