Antihemolytic and antioxidant activities of aerial parts extracts of Zygophyllum album L. and Globularia alypum L. from Algeria

Abstract

The main intrest of this study was to evaluate the antihemolytic and the antioxidant activities of the aqueous extracts of Globularia alypum and Zygophyllum album prepared by three different methods: decoction, infusion and maceration. Phytochemical tests, total phenolic, flavonoid and tannins contents of the different aqueous extracts were determined. DPPH radical scavenging assay and Ferric Reducing Antioxidant Power (FRAP) were employed for the evaluation of the antioxidant activity. Whereas, the antihemolytic activity was carried out by the evaluation of the protective effect of the various prepared extracts on red blood cells against the radical attack induced by 2,2′-azobis(2-amidinopropane) di-hydrochloride (AAPH). The aqueous extract of Globularia alypum prepared by decoction showed the highest level of total phenolic contents (196.52± 10.76 µg GAE/mg Extract). These findings confirmed the remarkably antioxidant properties of this plant with DPPH scavenging activity and FRAP test. A concentration of 160 µg/ml of this extract showed a protective effect against hemolysis induced by AAPH radical. Hence, Zygophyllum album extracts demonstrated lower level of total phenolic contents (30.41 ± 2.01 µg GAE/mg Extract) and lower antioxidant and antihemolytic activities. Globularia alypum extracts presented a powerful antioxidant and antihemolytic activities especially for the decoction extract whereas Zygophyllum album revealed a weaker protective effect except its maceration extract.

HPLC-DAD Analysis and Investigation of Biological Properties of the Leaves of Globularia alypum (L.), Infusion Extract

“…Two methods were used to quantify this activity: the ferric reducing antioxidant power (FRAP) and the DPPH radical scavenging assay. The data obtained indicated that G. alypum exhibited significant concentrations of scavenging activity: IC50 223.04 ± 0.73 to 85.37 ± 1.48 µg/mL and EC50 0.31 ± 0.01 to 0.68 ± 0.19 mg/mL [19]. The variety seen in the antioxidant activities of G. alypum might likely be explained by the experimental setup, the methods employed, and the utilization of plants from various geographical regions.…”

“…Nowadays, many researchers around the world are working on plant extracts to develop new antimicrobial agents with enhanced safety and efficiency [15][16][17][18][19]. The prime objective of ethnopharmacology is to identify plants for medicinal importance with minimal side effects.…”