Antihyperglycemic Activity of Charantin Isolated From Fruits of Momordica Charantia Linn.

Abstract

In recent years, there has been an increased interest in the development of alternative medicines for maintaining glucose homeostasis in diabetes, specifically by screening plant extracts as well as their isolated compounds for their ability to delay or prevent carbohydrate metabolism and absorption. The objective of present study was to undertake in-vitro and in-vivo studies to generate a stronger biochemical rationale for the management of post prandial hyperglycaemia (PPHG) with charantin. In this study, charantin (β-sitosteryl glucoside) was isolated from Momordica charantia Linn. by normal phase column chromatography and screened for the inhibition of key enzymes related to carbohydrate metabolism. Charantin showed mild αamylase (IC50 2.71± 0.21 mg/mL) and strong α-glucosidase (IC50 1.82±0.15 mg/mL) inhibitory activity. The positive in-vitro enzyme inhibition tests paved way for confirmatory in-vivo studies. The in-vivo studies demonstrated that charantin (20 mg/kg, b.w.) given orally significantly (P<0.01) reduced area under curve (AUC) in mice when challenged with oral administration of starch and sucrose separately. The reduction in AUC by charantin was comparable to that of acarbose (10 mg/kg, b.w., p.o.). These studies indicated the potential of charantin to prevent PPHG by inhibition of α-amylase and α-glucosidase enzymes.

Momordica charantia Suppresses Inflammation and Glycolysis in Lipopolysaccharide-Activated RAW264.7 Macrophages

“…Charantin is one of the key bioactive compounds of MC. However, charantin is known for its anti-diabetic activity [ 16 , 17 , 18 ], and its anti-inflammatory potential has yet to be elucidated. The anti-inflammatory activity of MC shows potential pharmacological value, but the abilities of the plant extract to regulate metabolism and inflammatory events in macrophages are not fully understood.…”

Применение В Народные Медицины Плоды Лекарственного Растения Momordica Charantia L

“…Rasm 1: Akarboz va charantin bilan davolanishdan keyin karbongidrat metabolizmi buzilgan sichqonlarning AUC [21].…”

“…Figure 1: AUC of mice with impaired carbohydrate metabolism after treatment with acarbose and charantin [21]. Experimental part.…”