Antihyperglycemic Activity of the Mahkota Dewa Leaf Extracts as an Alpha-Glucosidase Inhibitor

Sugiwati, Sri; Setiasih, Siswati; Afifah, Efy

doi:10.7454/msk.v13i2.364

Cited by 26 publications

(38 citation statements)

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“…A similar observation from a glucose tolerance test with extracts from young and old leaves and fruits of P . macrocarpa conducted in non-diabetic animals was reported by Sugiwati and co-workers [9,10]. Natural products with properties such as those identified in this study have the capacity to delay carbohydrate absorption into the blood stream by reversibly inhibiting the activity of carbohydrate digesting enzymes in the small intestinal brush border that are responsible for the breakdown of oligosaccharides and disaccharides into monosaccharides suitable for absorption [23].…”

Section: Discussionmentioning

confidence: 66%

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In vitro and in vivo effects of standardized extract and fractions of Phaleria macrocarpa fruits pericarp on lead carbohydrate digesting enzymes

Ali

Atangwho

Kuar

et al. 2013

BMC Complement Altern Med

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BackgroundOne vital therapeutic approach for the treatment of type 2 diabetes mellitus is the use of agents that can decrease postprandial hyperglycaemia by inhibiting carbohydrate digesting enzymes. The present study investigated the effects of bioassay-guided extract and fractions of the dried fruit pericarp of Phaleria macrocarpa, a traditional anti-diabetic plant, on α-glucosidase and α-amylase, in a bid to understand their anti-diabetic mechanism, as well as their possible attenuation action on postprandial glucose increase.MethodsMethanol extract (ME), obtained by successive solvent extraction, its most effective liquid-liquid n-butanol fraction (NBF) and the flash column chromatographic sub-fraction (SFI), were evaluated for in vitro α-glucosidase (yeast) and α-amylase (porcine) activity inhibition. Furthermore, confirmatory in vivo tests were carried out in streptozotocin-induced diabetic rats (SDRs) using oral glucose, sucrose and starch tolerance tests.ResultsAt the highest concentration employed (100 μg/ml), NBF showed highest inhibition against α-glucosidase (75%) and α-amylase (87%) in vitro (IC50 = 2.40 ± 0.23 μg/ml and 58.50 ± 0.13 μg/ml, respectively) in a dose-dependent fashion; an effect found to be about 20% higher than acarbose (55%), a standard α-glucosidase inhibitor (IC50 = 3.45 ± 0.19 μg/ml). The ME and SFI also inhibited α-glucosidase (IC50 = 7.50 ± 0.15 μg/ml and 11.45 ± 0.28 μg/ml) and α-amylase (IC50 = 43.90 ± 0.19 μg/ml and 69.80 ± 0.25 μg/ml), but to a lesser extent. In in vivo studies with diabetic rats, NBF and SFI effectively reduced peak blood glucose (PBG) by 15.08% and 6.46%, and the area under the tolerance curve (AUC) by 14.23% and 12.46%, respectively, after an oral sucrose challenge (P < 0.05); thereby validating the observed in vitro action. These reduction effects on PBG and AUC were also demonstrated in glucose and starch tolerance tests, but to a lesser degree.ConclusionsThese findings reveal that P. macrocarpa can attenuate hyperglycaemia in both in vitro and in vivo conditions by potently inhibiting carbohydrate hydrolysing enzymes, making it a viable plant for sourcing natural compounds for the management of type 2 diabetes mellitus.

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Section: Discussionmentioning

confidence: 66%

“…Earlier exploratory studies with young and old fruit extracts of P . macrocarpa on non-diabetic rats [9], and later with young and old leaf extracts [10] suggested that P . macrocarpa possessed possible α-glucosidase inhibitory activity.…”

Section: Introductionmentioning

confidence: 99%

In vitro and in vivo effects of standardized extract and fractions of Phaleria macrocarpa fruits pericarp on lead carbohydrate digesting enzymes

Ali

Atangwho

Kuar

et al. 2013

BMC Complement Altern Med

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“…The resulting yellow color is an indicator of the inhibitor ability to inhibit the reaction. When the ability of inhibitor to inhibit α-glucosidase work is greater, the resulting yellow color of the solution will be paler compared to the solution without inhibitor [17]. Inhibition of carbohydrate hydrolysis enzymes such as α-glucosidase become an important strategy in the control of blood glucose levels in the therapeutics of type 2 diabetes.…”

Section: Secondary Metabolites Screeningmentioning

confidence: 99%

Antidiabetic potential and secondary metabolites screening of mangrove gastropod Cerithidea obtusa

Cahyani¹,

Purwaningsih²

2015

J Coast Life Med

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“…This reaction would produce p-nitrophenol which has yellow color. The p-nitrophenol obtained from this reaction was then measured for its absorbance at 400 nm wavelength [27]. The enzymatic mixing reaction of α-glucosidase can be observed in Table 1.…”

Section: Inhibitory Activity Toward α-Glucosidase Assaymentioning

confidence: 99%

“…This crude extract was then heated using temperature of 70, 80, 90, and 100°C for 10 min. The observed parameters consist of yield, inhibition toward α-glucosidase [27], total phenolic content [28], and total tannins [29].…”

Section: Preparation Of Mangrove R Mucronata Fruit Powdermentioning

confidence: 99%

The Effect of Heating Temperature on Inhibitory Activity of Mangrove Rhizophora Mucronata Fruit Extract Toward Α-Glucosidase

Hardoko

Sasmito

Puspitasari

et al. 2018

Asian J Pharm Clin Res

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Objectives: The aim of this research was to determine the effect of heating temperature of mangrove Rhizophora mucronata fruit extract toward its inhibition activity of α-glucosidase. Methods:Research method used was an experimental method with the treatment of heating temperature (70°C, 80°C, 90°C, and 100°C) of crude tannin extract of mangrove R. mucronata fruit on inhibition activity toward α-glucosidase.Results: IC 50 of unheated extract and extracts that were heated at 70°C, 80°C, 90°C, and 100°C are adalah 3.38 ppm, 2.79 ppm, 2.97 ppm, 3.19 ppm, and 3.52 ppm, respectively. Heating until 100°C only decreased its inhibition activity of α-glucosidase of about 3.87% compared to unheated extract. Mangrove R. mucronata fruit extract contains phytochemical compounds, i.e., alkaloids, saponin, flavonoids, tannin, and terpenoids. IC 50 of this extract is lower compared to IC 50 of acarbose (antidiabetic medicine), which as IC 50 value of 13.27 ppm. Conclusion:Mangrove R. mucronata fruit extract is quite heat resistant and is potential to be an antidiabetic functional food.

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Antihyperglycemic Activity of the Mahkota Dewa Leaf Extracts as an Alpha-Glucosidase Inhibitor

Cited by 26 publications

References 0 publications

In vitro and in vivo effects of standardized extract and fractions of Phaleria macrocarpa fruits pericarp on lead carbohydrate digesting enzymes

In vitro and in vivo effects of standardized extract and fractions of Phaleria macrocarpa fruits pericarp on lead carbohydrate digesting enzymes

Antidiabetic potential and secondary metabolites screening of mangrove gastropod Cerithidea obtusa

The Effect of Heating Temperature on Inhibitory Activity of Mangrove Rhizophora Mucronata Fruit Extract Toward Α-Glucosidase

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