Antihyperglycemic and Antilipidemic Properties of a Tea Infusion of the Leaves from Annona cherimola Miller on Streptozocin-Induced Type 2 Diabetic Mice

Abstract

The antihyperglycemic and antilipidemic effects of the tea infusion extracts of leaves from Annona cherimola Miller (IELAc-0.5, IELAc-1.5, and IELAc-3.0) were evaluated on normoglycemic (NG) and streptozocin-induced diabetic (STID) mice. In the acute test, IELAc-1.5 at 300 mg/kg bodyweight (bw) exhibited antihyperglycemic activity on STID mice since the first hour of treatment. Then, its antidiabetic potential was analyzed in a subchronic evaluation. IELAc-1.5 was able to reduce the blood glucose level, glycated hemoglobin (HbA1c), cholesterol (CHO), and triglycerides (TG); high-density lipoprotein (HDL) showed an increase at the end of treatment. IELAc-1.5 did not modify the urine profile at the end of the evaluation, and neither toxicity nor macroscopic organ damage were observed in acute and subchronic assays. In addition, a major flavonol glycoside present in the tea infusion extracts was identified using high-performance liquid chromatography with diode array detection (HPLC-DAD). The analysis of the tea infusion extracts by HPLC revealed that rutin was the major component. This study supports the use of tea infusions from Annona cherimola for the treatment of diabetes and suggests that rutin could be responsible, at least in part, for their antidiabetic properties.

Antidiabetic and Toxicological Effects of the Tea Infusion of Summer Collection from Annona cherimola Miller Leaves

“…The aqueous extracts of Annona muricata , A. stenophylla , and A. macroprophyllata have shown antidiabetic, antioxidant, antilipidemic, and antinociceptive effects that are attributed to the presence of phenolic compounds in their leaves [ 34 , 35 , 36 , 37 ]. The antidiabetic properties of extracts from Ac have also been studied in previous publications [ 25 ] and it has been established that making a tea infusion from the leaf powder is a good way to produce the antilipidemic effect [ 38 ]; therefore, this extract was used in the present study. Nevertheless, no reports showing the histological changes induced by the treatment with this extract or changes in the effect related to the harvest period of the leaves have been studied.…”

Traditional Uses, Phytochemistry and Pharmacological Activities of Annonacae

“…Finally, a dose of 100 mg/kg of both methanolic extract and the residual fractions of A. reticulata leaves decreased blood glucose levels from 432.33 to 371.67 mg/dL and 417.83 to 402.50 mg/dL, respectively, in streptozotocin-induced diabetic rats [ 181 ]. A. cherimola leaf extract was also found to decrease HbA1c by 7% and lead to a significant decrease in urine glucose over a 28-day subchronic study in streptozotocin-induced diabetic mice [ 182 ]. These studies support the traditional use of Annona species as antidiabetics, suggesting that further identification of the active constituent(s) with antidiabetic properties and clinical studies of a longer duration are warranted.…”

“…Administration of an extract of A. muricata extracts resulted in reductions in the serum total cholesterol, low-density lipoprotein cholesterol and triglycerides in diabetic rats [ 183 ]. The tea infusion of leaves from A. cherimola (1.5 g) also elicited a reduction in the total cholesterol, triglycerides, and low-density lipoprotein by 15.4, 21.9 and 63.2%, respectively, in streptozotocin-induced diabetic rats [ 182 ].…”

Current Situation and Perspectives of Fruit Annonaceae in Mexico: Biological and Agronomic Importance and Bioactive Properties

“…Various studies on anti-hyperglycemic effects are reported for Annona species. The anti-hyperglycemic activity of A. squamosa leaves, roots, and peel extracts have been evaluated in hyperglycemic or diabetic models [ 153 , 154 , 155 , 156 , 157 , 158 , 159 , 160 , 161 , 162 , 163 ]. Table 4 shows that leaves have been studied more than other tissues.…”