Antihyperglycemic and hypoglycemic activities ofPhyllanthus debilisaqueous plant extract in mice

Wanniarachchi, Kasuni K.; Peiris, L.D.C.; Ratnasooriya, W.D.

doi:10.1080/13880200802435754

Cited by 14 publications

(11 citation statements)

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“…It was observed that AEF exerted its hypoglycemic effects through none dose-dependant manner with the mid dose producing pronounced hypoglycaemic effect. Further, the extract did produce a delayed hypoglycemic effect after 4 h and such delayed activity had been reported previously in plants such as Kokoona zeylanica L. [24], Strychnos henningsii L. [25] and Phylanthus debilis L. [26]. High dose of the extract exhibiting less hypoglycemic activity than the mid dose may be due to receptor down regulation activity of the drug thus decreasing the number of receptors for a drug or presence of other phytochemical substances [27].…”

Section: Discussionsupporting

confidence: 75%

Hypoglycemic and hypolipidemic activities of aqueous extract of flowers from Nycantus arbor-tristis L. in male mice

Rangika

Dayananda

Peiris

2015

BMC Complement Altern Med

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BackgroundBoiled aqueous extract of flowers (AEF) from Nyctanthes arbor-tristis L. are used in Sri Lankan traditional Ayruvedic Medicine to manage diabetes mellitus. AEF has widely been used as a folk medicine for the treatment of various ailments due to its therapeutic activity. However, little is known concerning therapeutic activity of the extract as well as its underline mechanisms and safety. Diabetes is known to increase low-density cholesterol and decrease high-density cholesterol thus triggering coronary diseases. Hence, the primary objective of the present study is to investigate the hypoglycemic and hypolipidemic activities of the AEF.MethodsAEF was prepared and male mice (n = 9 group) were gavaged either with 250, 500 and 750 mg/kg of AEF or distilled water (DW). Subsequently, fasting and random blood glucose concentrations were determined. To investigate mechanisms of actions of AEF, animals were orally administered with 500 mg/kg or the vehicle (DW) and glucose tolerance was performed before and after glucose challenge. For further studies, in vitro alpha-amylase assay and glucose absorption from the gastrointestinal tract were performed using 500 mg/kg of the extract. Additionally, glycogen content in the liver and skeletal muscles, a complete lipid profile assay, and toxicological and biochemical parameters were conducted after a chronic study.ResultsFive hundred mg/kg and 750 mg/kg of AEF significantly (p < 0.01) reduced fasting blood glucose levels respectively by 49 and 39 % at 4 h post-treatment, while 500 mg/kg of AEF also decreased the random blood glucose level significantly (p <0.01) by 32 % at 4 h post-treatment. AEF significantly inhibited glucose absorption by 85 % from the intestine and increased diaphragm uptake of glucose by 64 %. The extract also exhibited inhibition (16.66 %) of alpha-amylase enzyme activity. It also decreased the level of total cholesterol (by 44.8 %), triglyceride (by 53 %) and increased (by 57 %) the high-density lipoprotein cholesterol. Treatment with AEF did not induce any overt signs of toxicity or hepatotoxicity.ConclusionResults the present study indicated that AEF possess hypoglycemic and hypolipdemic properties. Therefore, AEF could be used as an alternative medicine in management of diabetes mellitus.

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Section: Discussionsupporting

confidence: 75%

Hypoglycemic and hypolipidemic activities of aqueous extract of flowers from Nycantus arbor-tristis L. in male mice

Rangika

Dayananda

Peiris

2015

BMC Complement Altern Med

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“…Such delayed effects may be due to active components in the extract that require a lag phase to reach sufficient concentrations at target sites. Similar delayed hypoglycaemic pattern was reported with plants such as Pylanthus debilis L ( Wanniarachchi, Peiris & Ratnasooriya, 2009 ), and Nycanthus arbor-tristis L ( Rangika, Dayananda & Peiris, 2015 ).…”

Section: Discussionsupporting

confidence: 81%

Hypoglycemic and hypolipidemic activity of aqueous leaf extract of Passiflora suberosa L

Sudasinghe

Peiris

2018

PeerJ

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Leaves of Passiflora suberosa L. (Family: Passifloraceae; common name: wild passion fruit, devil’s pumpkin) are used in Sri Lankan traditional medicine for treating diabetes. The present study investigated the in vivo ability of P. suberosa leaves to manage blood sugar status and associated cholesterol levels. Mechanisms of action and toxicity were also determined. Phytochemical screening of aqueous extracts of P. suberosa leaves and carbohydrate content of the leaves were determined according to previously published methods. In two group of male mice (n = 9), effects on fasting and random blood glucose levels (BGLs) of different acute doses (0, 25, 50, 100 and 200 mg/kg) of the aqueous leaf extract (ALE) were evaluated at 1, 3, and 5 h post-treatment. In another set of mice, the fasting BGL was evaluated following treatment of 0 or 50 mg/kg ALE (dose prescribed in traditional medicine) for 30 consecutive days. The lipid profile, some mechanism of ALE action (diaphragm glucose uptake, glycogen content in the liver and skeletal muscles) and its toxicity (behavioural observation, food and water intake, hepatoxicity) were also assessed following 30-day treatment. However, sucrose and glucose tolerance tests and intestinal glucose uptake were conducted to determine portion of mechanisms of action following single dose of 50 mg/kg ALE. Phytochemical screening revealed the presence of alkaloids, unsaturated sterols, triterpenes, saponins, flavonoids, tannins and proanthocyanidins. Carbohydrate content of the leaves was 12.97%. The maximum hypoglycemic effect was observed after 4 h of 50 and 100 mg/kg ALE administration. The extract decreased fasting BGL (18%) following an oral sucrose challenge and inhibited (79%) glucose absorption from the intestine. Correspondingly, the levels of glycogen in the liver (61%) and in the skeletal muscles (57%) were found be higher than that of the control group. The levels of total cholesterol (17%) and tri-glyceraldehyde levels (12%) found to be reduced in treated groups. Furthermore, no significant toxic effects were observed in treated groups. The present results suggest that the leaves of P. suberosa can be used to manage blood glucose and cholesterol levels. Isolation of active compounds are recommended for further analysis.

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“…Eighteen rats (Wanniarachchi et al, 2009) were weighed, numbered, and randomly divided into 3 groups (NCG1, CME-PM-2000, PCG1) of 6 animals each and each rat was fed with a dosage of 2000 mg/kg body weight for the purpose of evaluation of acute toxicity, anti-hypercholectrolemic activity and 6 rats in five group (NCG2, CME-PM-400, CME-PM-800, CME-PM-1200, PCG2) included in the determinations of dose dependant response ( Table 1).…”

Section: Experimental Designmentioning

confidence: 99%

Effect of Phyllanthus maderaspatensis L. crude methanolic extract on diet induced hypercholesterolemia in Wistar albino rats (Mus norvegicus albinus)

Dissanayaka¹,

Nilakarawasam²,

Somaratne³

et al. 2019

Ceylon J. Sci.

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Present experiment was aimed to assess the diet induced hypocholesterolemic activity of crude methanolic extracts of Phyllanthus maderaspatensis L. on Wistar rats. The crude whole plant methanolic extract was administered orally at the dosage of 2000 mg/kg body weight per day, to diet induced hypercholesterolemic Wistar albino rats for 14 days to assess the acute toxicity and hypercholesterolemic activity. Lower concentrations were used since no toxicity found during the acute toxicity experiment. Dosages of Methanolic extracts of 400mg, 800 mg and 1200 mg/ kg body weight per day were orally administered to rats for 42 days whilst total cholesterol, triglyceride, were studied. Hardly any acute toxicity signs and liver toxicity were observed with the extract of 2000 mg/kg. The same concentration indicated a reduction of total cholesterol and triglyceride during 14 days. The groups treated with three lower doses of extract exhibited dose dependent negative response with total cholesterol and triglyceride. Results indicated capability of the crude methanolic extract of P. maderaspatensis L. in total cholesterol and triglyceride in diet induced hypercholestrolemic Wistar rats.

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Antihyperglycemic and hypoglycemic activities ofPhyllanthus debilisaqueous plant extract in mice

Cited by 14 publications

References 14 publications

Hypoglycemic and hypolipidemic activities of aqueous extract of flowers from Nycantus arbor-tristis L. in male mice

Hypoglycemic and hypolipidemic activities of aqueous extract of flowers from Nycantus arbor-tristis L. in male mice

Hypoglycemic and hypolipidemic activity of aqueous leaf extract of Passiflora suberosa L

Effect of <i>Phyllanthus maderaspatensis</i> L. crude methanolic extract on diet induced hypercholesterolemia in Wistar albino rats (<i>Mus norvegicus albinus</i>)

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