Antihyperglycemic, hypolipidemic, hepatoprotective and antioxidative effects of dietary clove (<i>Szyzgium aromaticum</i>) bud powder in a high-fat diet/streptozotocin-induced diabetes rat model

Adefegha, Stephen A.; Oboh, Ganiyu; Adefegha, Omowunmi Monisola; Boligon, Aline Augusti; Athayde, Margareth Linde

doi:10.1002/jsfa.6617

Cited by 111 publications

(67 citation statements)

References 91 publications

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“…Similarly, the total flavonoid content of methanol (2.25± 0.3mg QE/g mg quercetin equivalent (QE)/g) was higher than those of ethanol (2.15 ± 0.2 mg QE/g) and ethyl acetate (2.04 ± 0.1mg QE/g) extracts but not significantly (P >0.05) different. The observed trend of results on total phenol and total flavonoid contents as shown in table 1 agrees with several reports on tropical vegetables with similar trends between the total phenol and flavonoid contents (Adefegha and Oboh, 2011;Eghdami and Sadegh, 2010;Kasote et al, 2015;Pavithra and Vadivukkarasi, 2015).…”

Section: Resultssupporting

confidence: 80%

“…Qualitative-quantitative analyses using HPLC-diode-array detector (DAD) was performed with a Shimadzu Prominence Auto Sampler (SIL-20A) HPLC system (Shimadzu, Kyoto, Japan), equipped with Shimadzu LC-20AT reciprocating pumps connected to a DGU 20A5 degasser with a CBM 20A integrator, SPD-M20A DAD and LC solution 1.22 SP1 software. Reverse phase chromatographic analyses were carried out under gradient conditions using C 18 column (4.6 mm x 150 mm) packed with 5μm diameter particles; the mobile phase was water containing 2% acetic acid (A) and methanol (B), and the composition gradient was: 5% of B until 2 min and changed to obtain 25%, 40%, 50%, 60%, 70% and 100% B at 10, 20, 30, 40, 50 and 80 min, respectively according to the method described by Adefegha et al (2014). The presence of nine antioxidants compounds was investigated, namely, gallic acid, caffeic acid, catechin, quercetin, quercitrin, rutin, kaempferol, luteolin and apigenin.…”

Section: Quantification Of Compounds By High Performance Liquidmentioning

confidence: 99%

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Comparative study on the phenolic content, antioxidant properties and HPLC fingerprinting of the leaf extracts of Clerodendrum volubile P. Beauv

Molehin¹,

Oloyede²,

Boligon³

2017

J App Pharma Sci

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The present study aimed to investigate the phenolic contents and antioxidant properties of the various leaf extracts (methanol, ethanol, ethyl acetate) of Clerodendrum volubile. The total phenol and total flavonoid contents of the extracts were determined. In vitro scavenging activities of the extracts were assessed against 1, 1-diphenyl-2 picrylhydrazyl (DPPH), 2, 2-azinobis (3-ethylbenzo-thiazoline-6-sulfonate (ABTS) radical scavenging ability. The reducing power ability and high-performance liquid chromatography-diode-array detector (HPLC-DAD) of the extracts were also determined. The results revealed that methanolic extract had the highest total phenol and total flavonoid contents in all the extracts. The methanol extract had the highest reducing power, DPPH and ABTS radicals scavenging activities, followed by ethanol and ethyl acetate extracts. The HPLC-DAD results revealed the presence of both phenolic acids (caffeic acid, gallic acid) and flavonoids (catechin, quercetin, rutin, quercitrin, kaempferol, luteolin, apigenin) as its major bioactive polyphenolics. The difference in scavenging potential of the extracts may be due to variation in the phytoconstituents extracted using various solvents. The result suggests that the methanol leaf extract of Clerodendrum volubile could serve as a potential source of antioxidants and can be explored as a therapeutic agent in free radical induced diseases.

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Section: Resultssupporting

confidence: 80%

Section: Quantification Of Compounds By High Performance Liquidmentioning

confidence: 99%

Comparative study on the phenolic content, antioxidant properties and HPLC fingerprinting of the leaf extracts of Clerodendrum volubile P. Beauv

Molehin¹,

Oloyede²,

Boligon³

2017

J App Pharma Sci

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“…For example, clove ( Syzygium aromaticum ) bud powder reduced blood glucose level in a high fat-induced diabetic rat [23]. A previous study revealed that eugeniin, the phytochemical compounds isolated from aqueous methanol extraction was attributed with the inhibition of rat intestinal maltase [24].…”

Section: Discussionmentioning

confidence: 99%

The inhibitory activity of herbal medicines on the keys enzymes and steps related to carbohydrate and lipid digestion

Sompong

Muangngam

Kongpatpharnich

et al. 2016

BMC Complement Altern Med

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BackgroundObesity and overweight are consistently associated with metabolic disorders including hyperglycemia and hyperlipidemia. Herbal medicines have been currently suggested as an alternative source of potentially useful antihyperglycemic, antihyperlipidemic, antioxidant activities. The objective of this study was to assess the in vitro inhibitory effects of eleven herbal medicines on carbohydrate and lipid digestive enzymes and the key steps of lipid digestion including the inhibition of micelle formation and the ability to bind bile acid. In addition, antioxidant activity of herbal medicines was also investigated.Methodsα-Glucosidase, pancreatic α-amylase, pancreatic lipase, and pancreatic cholesterol esterase inhibitory activities of aqueous extract of herbal medicines were measured using the enzymatic colorimetric assay. The formation of cholesterol micelles was determined using the cholesterol assay kit. The bile acid binding was measured using the colorimetric assay. Antioxidant activities were assessed by using four methods including Trolox equivalent antioxidant capacity (TEAC), oxygen radical absorbance capacity ORAC), superoxide radical scavenging activity (SRSA), and hydroxyl radical scavenging activity (HRSA).ResultsThe extracts (1 mg/mL) markedly inhibited intestinal maltase (5.16 − 44.33 %), sucrase (1.25–45.86 %), pancreatic α-amylase (1.75–12.53 %), pancreatic lipase (21.42–85.93 %), and pancreatic cholesterol esterase (2.92–53.35 %). The results showed that all extracts exhibited the inhibitory activity against pancreatic lipase with the IC50 values ranging from 0.015 to 4.259 mg/mL. In addition, the incorporation of cholesterol into micelles was inhibited by the extracts (6.64–33.74 %). The extracts also bound glycodeoxycholic acid (9.9–15.08 %), taurodeoxycholic acid (12.55–18.18 %), and taurocholic acid (11.91 − 18.4 %). Furthermore, the extracts possessed various antioxidant activities including the TEAC values (0.50 − 4.70 μmol trolox/mg dried extract), the ORAC values (9.14–44.41 μmol trolox/mg dried extract), the SRSA (0.31 − 18.82 mg trolox/mg dried extract), and the HRSA (0.05–2.29 mg trolox/mg dried extract). The findings indicated that Syzygium aromaticum, Phyllanthus amarus, Thunbergia laurifolia were the effective promising antihyperglycemic and antihyperlipidemic agents. Statistical analysis demonstrated strong positive significant correlations between the contents of phenolic compounds and % inhibition of pancreatic lipase (r = 0.885, p < 0.001), % inhibition of pancreatic cholesterol esterase (r = 0.761, p < 0.001), and the TEAC value (r = 0.840, p < 0.001). Furthermore, there was a strongly positive correlation between the TEAC value and % inhibition of pancreatic cholesterol esterase (r = 0.851, p < 0.001) and % inhibition of pancreatic lipase (r = 0.755, p < 0.001).ConclusionsThree herbal medicines including Syzygium aromaticum, Thunbergia laurifolia, and Phyllanthus amarus markedly inhibited the intestinal α-glucosidase, pancreatic α-amylase, pancreatic lipase, and pancreat...

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“…The bioassay was carried out according by the modified method described by Adefegha et al [7]. Fifty Male Wistar rats weighing 135-147 g were purchased from the Department of Veterinary Medicine, University of Ibadan, Nigeria.…”

Section: Experimental Designmentioning

confidence: 99%

“…After two weeks of acclimatization, diet formulation was carried out according to the method described by Adefegha et al [7]. The rats were placed on three dietary regimens namely:…”

Section: Diets and Experimental Groupsmentioning

confidence: 99%

Alligator pepper/Grain of Paradise ( Aframomum melegueta ) modulates Angiotensin-I converting enzyme activity, lipid profile and oxidative imbalances in a rat model of hypercholesterolemia

et al. 2016

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Antihyperglycemic, hypolipidemic, hepatoprotective and antioxidative effects of dietary clove (Szyzgium aromaticum) bud powder in a high-fat diet/streptozotocin-induced diabetes rat model

Abstract: The results suggest that the clove bud diet may attenuate hyperglycemia, hyperlipidemia, hepatotoxicity and oxidative stress in the type 2 diabetic condition.

Cited by 111 publications

References 91 publications

Comparative study on the phenolic content, antioxidant properties and HPLC fingerprinting of the leaf extracts of Clerodendrum volubile P. Beauv

Comparative study on the phenolic content, antioxidant properties and HPLC fingerprinting of the leaf extracts of Clerodendrum volubile P. Beauv

The inhibitory activity of herbal medicines on the keys enzymes and steps related to carbohydrate and lipid digestion

Alligator pepper/Grain of Paradise ( Aframomum melegueta ) modulates Angiotensin-I converting enzyme activity, lipid profile and oxidative imbalances in a rat model of hypercholesterolemia

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