Antihyperlipidemic and Antioxidant Effects of Extracts from Pleurotus citrinopileatus

Abstract

Pleurotus citrinopileatus is a popular edible mushroom which is physiologically active in both humans and animals. In the study we investigate the effects of this mushroom on hyperlipidemic hamster rats. Four dietary forms of the mushroom were created as follows. The powdered dry fruiting body, hot-water extract, and two kinds of elutes were obtained, from ethyl acetate extract and methanol extract, respectively, in different mixed proportion solvents over silica gel column chromatography (referred to as EAE and MOE, respectively). They were tested at different dosages as a supplement to a high-fat diet in hyperlipidemic rats. Serum triglycerides and total cholesterol levels were significantly lower in groups supplemented with the highest dosages of EAE and MOE (0.5 g/kg, body weight daily) as compared with the control groups that received no mushroom additive. High-density lipoprotein levels in these same two experimental groups were also significantly higher than those in the negative control group. The tested rats that were fed with EAE had the highest serum glutathione peroxidase and superoxide dismutase activity, and those with the MOE and EAE had the highest DPPH free radical scavenging activities and ferric-reducing abilities, tested in vitro. The major constituents of MOE and EAE were identified as ergosterol and nicotinic acid, respectively. P. citrinopileatus extracts may have a significant antihyperlipidemia effect. Furthermore, antioxidant activities and antihyperlipidemic effects of MOE and EAE seemed to display similar tendencies.

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“…As with other studies on mushroom extracts 26,27,32 , the reducing power abilities of mushroom extracts increased with concentration (Fig. 2).…”

Development of a Novel Methodology for the Analysis of Ergosterol in Mushrooms

“…This sterol is becoming an important research topic because its content is usually associated with structural and growing fungal characteristics, such as maturation, hyphal formation and sporulation (Villares et al 2012), being also dependent on the fungal species (Phillips et al 2011). From the bioactivity point of view, ergosterol has great importance since it can be converted into vitamin D 2 after photolysis and thermal rearrangement (Villares et al 2012), and was reported as having health-promoting properties such as antioxidant (Shao et al 2010), anti-inflammatory (Kuo et al 2011) and antihyperlipidemic (Hu et al 2006) activities, being also involved in the activated expression of specific defense genes (Lochman and Mikes 2005).…”

“…In fact, the health-promoting properties of ergosterol, including the 13 antioxidant (Shao et al 2010), anti-inflammatory (Kuo et al 2011) antihyperlipidemic (Hu et al 2006), genetic expression control (Lochman and Mikes 2005) and antitumoral activities (Takaku et al 2001;Yazawa et al 2000), are closely related to the uptake doses. Furthermore, it will be important to understand how its concentration varies during the main fungi development stages.…”

Comparative Effects of the Leaves of <i>Gongronema latifolium</i> and <i>Vernonia amygdalina</i> Incorporated Diets on the Lipid Profiles of Rats

“…There is a need for a drug that may control both hypercholestrolaemia and hypertriglyceridaemia. Hence the nutritional inhibition or reduction of hypercholestrolaemic conditions is considered to be an important therapeutic approach and efforts have been made to identify medicinal plants with these functions 7,[13][14][15] .…”